Critical role of TRPC6 channels in VEGF-mediated angiogenesis

Ge, Ruowen; Tai, Yilin; Sun, Yuanyuan; Zhou, Kesong; Yang, Shenglin; Cheng, Tian-Lin; Zou, Qifei; Shen, Feng; Wang, Yizheng

doi:10.1016/j.canlet.2009.03.023

Cited by 115 publications

(97 citation statements)

References 41 publications

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“…TRPC channels have been shown to participate in in vitro angiogenesis, in particular TRPC6 (17,18). On EA.hy926 cells, TRPC6 knockdown slightly reduced tube formation, a marginal effect compared with the important effect of TRPC3, TRPC4, and TRPC5 knockdown.…”

Section: Discussionmentioning

confidence: 99%

“…Angiogenesis, the formation of new blood vessels from preexisting ones, is a complex and multistep process that comprises migration, proliferation, and reorganization of endothelial cells (15). Ca 2ϩ signals are pivotal for many steps that are taking place during angiogenesis (16), and recent data reported that TRPC6 is an important player in angiogenesis (17,18) whereas others revealed that angiogenesis is severely impaired after Orai1 knockdown (19). In a recent paper, we showed that TRPC1, TRPC3, TRPC4, TRPC5, and TRPC6 isoforms are expressed, together with STIM1 and Orai1, on EA.hy926 cells (20), an endothelial cell line derived from human umbilical vein endothelial cells (HUVECs) fused with human lung adenocarcinoma cell line A549 (21).…”

mentioning

confidence: 99%

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Transient Receptor Potential Canonical Channels Are Required for in Vitro Endothelial Tube Formation

Antigny¹,

Girardin²,

Frieden³

2012

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Transient Receptor Potential Canonical Channels Are Required for in Vitro Endothelial Tube Formation

Antigny¹,

Girardin²,

Frieden³

2012

Journal of Biological Chemistry

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“…In this context, the recent interest in human 'transportome' involvement in tumour vascularization is a promising field, because several members are activated downstream of the recruitment of VEGF receptors. For example, whereas the interference with the bulk VEGF signalling alters the activity of a multitude of different cells and functions, targeting TRPC6 or Orai1 may affect only EC migration and proliferation [31,34,44,53,92], whereas TRPC1 and STIM1 may selectively influence vascular permeability [56][57][58]61].…”

Section: Resultsmentioning

confidence: 99%

“…For example, TRPC6 channels targeting could affect VEGF release from tumour cells as well as EC migration and tumour vascularization [31,44,53].…”

Section: Resultsmentioning

confidence: 99%

“…HMEC, human microvascular EC; HPAEC, human pulmonary artery EC; HUVEC, human umbilical vein EC; EA.hy926, EC line derived from HUVEC fused with human lung adenocarcinoma cell line A549; PAEC, porcine aortic endothelial cells; BTEC, tumour-derived EC from breast carcinoma; H5VEC, heart endothelioma (H5V) EC; MAEC, mouse aortic EC; EPC, endothelial progenitor cells; RCC-EPC, EPC isolated from renal carcinoma patients; Numbers in parenthesis indicate the respective reference number. EPC [30] HMEC [31] BTEC [32] RCC-EPC [29] HUVEC [33 -35] HUVEC [36] HUVEC [37] HUVEC [38] HUVEC [36,39] MAEC from KO mice [40] HMEC [41,42] EPC [43] survival and proliferation RCC-EPC [29] EPC [30] HMEC [31] HUVEC [44] PAEC [45] H5V EC [46] HUVEC [40] HUVEC, HMEC [41,55] EPC [43] permeability HMEC,HUVEC [56 -58] HPAEC [59] Frog mesenteric microvessels [60] H5V EC [46] HUVEC [61] in vivo angiogenesis zebrafish [62] CAM [44] collateral growth [45] HERG-1-Retinoblastoma [63] EAG1-Xenograft in SCID mice and human osteosarcoma [27,28] CAM [54] xenograft in nude mice [38] Rabbit cornea [64] human mammary carcinoma, glioblastoma [65,66] AQP1 KO mice and C57BL/ 6 mice …”

Section: Transient Receptor Potential Proteins and Stim1-orai1 Complexmentioning

confidence: 99%

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Functional properties of ion channels and transporters in tumour vascularization

Fiorio

Munaron

2014

Phil. Trans. R. Soc. B

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Vascularization is crucial for solid tumour growth and invasion, providing metabolic support and sustaining metastatic dissemination. It is now accepted that ion channels and transporters play a significant role in driving the cancer growth at all stages. They may represent novel therapeutic, diagnostic and prognostic targets for anti-cancer therapies. On the other hand, although the expression and role of ion channels and transporters in the vascular endothelium is well recognized and subject of recent reviews, only recently has their involvement in tumour vascularization been recognized. Here, we review the current literature on ion channels and transporters directly involved in the angiogenic process. Particular interest will be focused on tumour angiogenesis in vivo as well as in the different steps that drive this process in vitro , such as endothelial cell proliferation, migration, adhesion and tubulogenesis. Moreover, we compare the ‘transportome’ system of tumour vascular network with the physiological one.

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Roles of Ion Transport in Control of Cell Motility

Stock

Ludwig

Hanley

et al. 2013

Comprehensive Physiology

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Cell motility is an essential feature of life. It is essential for reproduction, propagation, embryonic development, and healing processes such as wound closure and a successful immune defense. If out of control, cell motility can become life-threatening as, for example, in metastasis or autoimmune diseases. Regardless of whether ciliary/flagellar or amoeboid movement, controlled motility always requires a concerted action of ion channels and transporters, cytoskeletal elements, and signaling cascades. Ion transport across the plasma membrane contributes to cell motility by affecting the membrane potential and voltage-sensitive ion channels, by inducing local volume changes with the help of aquaporins and by modulating cytosolic Ca(2+) and H(+) concentrations. Voltage-sensitive ion channels serve as voltage detectors in electric fields thus enabling galvanotaxis; local swelling facilitates the outgrowth of protrusions at the leading edge while local shrinkage accompanies the retraction of the cell rear; the cytosolic Ca(2+) concentration exerts its main effect on cytoskeletal dynamics via motor proteins such as myosin or dynein; and both, the intracellular and the extracellular H(+) concentration modulate cell migration and adhesion by tuning the activity of enzymes and signaling molecules in the cytosol as well as the activation state of adhesion molecules at the cell surface. In addition to the actual process of ion transport, both, channels and transporters contribute to cell migration by being part of focal adhesion complexes and/or physically interacting with components of the cytoskeleton. The present article provides an overview of how the numerous ion-transport mechanisms contribute to the various modes of cell motility.

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Critical role of TRPC6 channels in VEGF-mediated angiogenesis

Cited by 115 publications

References 41 publications

Transient Receptor Potential Canonical Channels Are Required for in Vitro Endothelial Tube Formation

Transient Receptor Potential Canonical Channels Are Required for in Vitro Endothelial Tube Formation

Functional properties of ion channels and transporters in tumour vascularization

Roles of Ion Transport in Control of Cell Motility

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