Critical role of Wat1/Pop3 in regulating the TORC1 signalling pathway in fission yeast S. pombe

Panigrahi, Lalita; Anjum, Simmi; Ahmed, Shakil

doi:10.1016/j.fgb.2022.103764

Cited by 4 publications

(2 citation statements)

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“…1µg of RNA was reverse transcribed using a cDNA synthesis kit (Thermo Scientific) followed by qRT-PCR with SYBR green master mix kit (Takara). The reactions were performed in triplicate using the Agilent Mx3000P qPCR system and analysis was performed essentially as described earlier (Panigrahi et al, 2023).…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

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Wat1/mLst8, a TOR complex protein regulates mitochondrial integrity and calcium ion homeostasis in fission yeastS. pombe

Anjum

Srivastava

Panigrahi

et al. 2023

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The mTOR complexes play a fundamental role in mitochondrial biogenesis and cellular homeostasis. Wat1, an ortholog of mammalian Lst8 is an important component of TOR complex and is essential for the regulation of downstream signaling. Earlier we reported the role of Wat1 in oxidative stress response. Here, we show that the inactivation of wat1 leads to respiratory defects and mitochondrial depolarization leading to decrease in ATP production. The confocal and electron microscopy in wat1Δ cells revealed the fragmented mitochondrial morphology implying its role in mitochondrial fission. Furthermore, we also showed its role in autophagy and the maintenance of calcium ion homeostasis. Additionally, tor2-287 mutant cells also exhibit defects in mitochondrial integrity indicating the TORC1-dependent involvement of Wat1 in the maintenance of mitochondrial homeostasis. The interaction studies of Wat1 and Tor2 with Por1 and Mmm1 proteins revealed a cross-talk between mitochondria and endoplasmic reticulum through the Mitochondria-associated membranes (MAM) and endoplasmic reticulum-mitochondria encounter structure (ERMES) complex, involving TORC1. Taken together, this study demonstrates involvement of Wat1/mLst8 in harmonizing various mitochondrial functions, redox status, and Ca2+ homeostasis.

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Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…Wat1, an ortholog of mammalian Lst8 is an important component of both TOR complexes TORC1 and TORC2 and regulates the downstream signaling (Ahamad et al, 2018, Panigrahi et al, 2023). Its role in genomic stability and microtubule integrity has also been reported (Verma et al, 2014; Ochotorena et al, 2001).…”

Section: Introductionmentioning

confidence: 99%