Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast

Kuroda, Kouichi; Hammer, Sarah K.; Watanabe, Yukio; López, José Montaño; Fink, Gerald R.; Stephanopoulos, Gregory; Ueda, Mitsuyoshi; Avalos, José L.

doi:10.1016/j.cels.2019.10.006

Cited by 36 publications

(56 citation statements)

References 84 publications

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“…These results suggest a Gaf1-dependent transcriptional program to adjust the metabolism of amino acids and other molecules, possibly to recycle nutrients under conditions that do not allow rapid proliferation. Similar gene-expression changes are mediated by budding yeast Gln3 and Gat1 under conditions of TORC1 inhibition (Kuroda et al, 2019;Scherens et al, 2006). Altogether, these findings indicate that Gaf1 regulates physiological changes supporting the growth arrest triggered by TORC1 inhibition.…”

Section: Gaf1-dependent Transcriptome Regulation Following Tor Inhibitionmentioning

confidence: 57%

The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1

et al. 2020

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Target of Rapamycin Complex 1 (TORC1) signaling promotes growth and aging. Inhibition of TORC1 leads to reduced protein translation, which promotes longevity. TORC1-dependent post-transcriptional regulation of protein translation has been well studied, while analogous transcriptional regulation is less understood. Here we screen fission yeast mutants for resistance to Torin1, which inhibits TORC1 and cell growth. Cells lacking the GATA factor Gaf1 (gaf1D) grow normally even in high doses of Torin1. The gaf1D mutation shortens the chronological lifespan of non-dividing cells and diminishes Torin1-mediated longevity. Expression profiling and genome-wide binding experiments show that upon TORC1 inhibition, Gaf1 directly upregulates genes for small-molecule metabolic pathways and indirectly represses genes for protein translation. Surprisingly, Gaf1 binds to and downregulates the tRNA genes, so it also functions as a transcription factor for RNA polymerase III. Thus, Gaf1 controls the transcription of both protein-coding and tRNA genes to inhibit translation and growth downstream of TORC1.

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Section: Gaf1-dependent Transcriptome Regulation Following Tor Inhibitionmentioning

confidence: 57%

The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1

et al. 2020

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“…boosts the yeast to the branched-chain alcohols tolerance (Kuroda et al 2019). The finding mentioned fits our hypothesis since BMA64-1A is the only strain with the downregulation of GLN3 (data not shown) and presents the highest EtOH tolerance here analyzed.…”

Section: Etoh Tolerancementioning

confidence: 99%

Long non-coding RNAs bind to proteins relevant to the ethanol tolerance in yeast: a systems biology view

et al. 2021

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The ethanol disturbs the cell cycle, transcription, translation, protein folding, cell wall, membranes, and many Saccharomyces cerevisiae metabolic processes. Long non-coding RNAs (lncRNAs) are regulatory molecules binding onto the genome or proteins. The number of lncRNAs described for yeast is still scarce, and little is known concerning their roles in the system. There is a lack of knowledge concerning how lncRNAs are responsive to the ethanol tolerance in yeast and whether they act in this tolerance. Hence, by using RNA-Seq data from S. cerevisiae strains with different ethanol tolerance phenotypes, we found the severe ethanol responsive lncRNAs. We modeled how they participate in the ethanol tolerance by analyzing lncRNA-protein interactions. The results showed that the EtOH tolerance responsive lncRNAs, in both higher tolerant and lower tolerant phenotypes, work on different pathways: cell wall, cell cycle, growth, longevity, cell surveillance, ribosome biogenesis, intracellular transport, trehalose metabolism, transcription, and nutrient shifts. In summary, lncRNAs seems to interconnect essential systems modules to overcome the ethanol stress. Finally, here we also found the most extensive catalog of lncRNAs in yeast.

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“…Yeast is a valuable microbial cell factory in biosynthesis of industrial products, such as biofuels, organic acids terpenoids, abscisic acid and flavonoids [1][2][3][4][5]. However, cell growth rate and product yield are limited in industrial fermentation processes, due to the issues such as heat shock, product toxicities and oxidative stress [6].…”

Section: Introductionmentioning

confidence: 99%

“…The mediator is a conserved transcriptional coactivation complex. Its component subunits of Med2p, Med3p, and Med15p can recruit on regulatory regions of prompter, which enables smooth expression of target genes involving heat shock protein (HSPs), protein homeostasis and trehalose synthesis (2). The number of new ribosomal proteins (RPs) and rRNAs do not match, resulting in the orphan r-proteins aggregate.…”

mentioning

confidence: 99%

“…Functional reaction to oxidant and Toxic molecule stress signals(1) The activation of Yap1p is dependent or not on ROS caused by oxidizing chemicals, it transits from cytoplasm to nucleus to stimulate the expression of oxidative stress responsive gene such as GSH, GSN, SOD2 and GLR1. And, Hyr1p or Ybp1p-Gpx3p complex can inhibit YAP1 output from nuclear (2). The unfolded protein response (UPR) signaling pathway mainly contains two regulators, i.e., the Ire1p and Hac1p.…”

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confidence: 99%

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Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

Dong¹,

Qin²,

Li³

et al. 2020

Preprint

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Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast

Cited by 36 publications

References 84 publications

The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1

The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1

Long non-coding RNAs bind to proteins relevant to the ethanol tolerance in yeast: a systems biology view

Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

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