CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons

Cha, Caihui; Zhang, Jifeng; Ji, Zhisheng; Tan, Minghui; Li, Sumei; Wu, Fengming; Chen, Keen; Gong, Sitang; Guo, Guoqing; Lin, Hongsheng

doi:10.1016/j.brainresbull.2016.06.008

Cited by 22 publications

(27 citation statements)

References 35 publications

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“…CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons (Cha et al . ). Thus, the enhanced expression of CRMP3 and CRMP4 mitigates the phenotype of crmp2 −/− mice.…”

Section: Discussionmentioning

confidence: 97%

“…Deletion of CRMP3 also negatively affects hippocampal CA1 dendritic organization and plasticity (Quach et al 2008), suggesting that CRMPs are important in maintaining cognitive functions. CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons (Cha et al 2016). Thus, the enhanced expression of CRMP3 and CRMP4 mitigates the phenotype of crmp2 À/À mice.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive behavioral study and proteomic analyses of CRMP2‐deficient mice

et al. 2016

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Collapsin response mediator protein 2 (CRMP2) plays a key role in axon guidance, dendritic morphogenesis and cell polarization. CRMP2 is implicated in various neurological and psychiatric disorders. However, in vivo functions of CRMP2 remain unknown. We generated CRMP2 gene-deficient (crmp2 À/À ) mice and examined their behavioral phenotypes. During 24-h home cage monitoring, the activity level during the dark phase of crmp2 À/À mice was significantly higher than that of wild-type (WT) mice. Moreover, the time during the open arm of an elevated plus maze was longer for crmp2 À/À mice than for WT mice. The duration of social interaction was shorter for crmp2 À/À mice than for WT mice. Crmp2 À/À mice also showed mild impaired contextual learning. We then examined the methamphetamine-induced behavioral change of crmp2 À/À mice. Crmp2 À/À mice showed increased methamphetamine-induced ambulatory activity and serotonin release. Crmp2 À/À mice also showed altered expression of proteins involved in GABAergic synapse, glutamatergic synapse and neurotrophin signaling pathways. In addition, SNAP25, RAB18, FABP5, ARF5 and LDHA, which are related genes to schizophrenia and methamphetamine sensitization, are also decreased in crmp2 À/À mice.Our study implies that dysregulation of CRMP2 may be involved in pathophysiology of neuropsychiatric disorders.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Comprehensive behavioral study and proteomic analyses of CRMP2‐deficient mice

et al. 2016

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“…Collapsin response mediator proteins (CRMPs) are a family of cytosolic phospho-proteins that regulate cytoskeletal dynamics during development and after injury (Alabed et al, 2007;Khazaei et al, 2014;Nagai et al, 2015Nagai et al, , 2016Tan et al, 2015). The CRMP4 family member has two splice isoforms, referred to as the long isoform of CRMP4 (CRMP4L) and the short isoform of CRMP4 (CRMP4S), and is an important neurodevelopmental molecule promoting axonal extension and dendrite branching (Quinn et al, 2003;Niisato et al, 2012;Khazaei et al, 2014;Tan et al, 2015;Cha et al, 2016). However, in the adult CNS, CRMP4-null mice exhibit enhanced neuronal regeneration and reduced inflammation following spinal cord injury, suggesting that CRMP4 impedes regeneration in this context (Nagai et al, 2015(Nagai et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury

Girouard

Simas

Hua

et al. 2020

eNeuro

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In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and cytoskeletal disassembly of the distal segment. Collapsin response mediator protein 4 (CRMP4) is a cytosolic phospho-protein that regulates the actin and microtubule cytoskeleton. During development, CRMP4 promotes growth cone formation and dendrite development. Paradoxically, in the adult CNS, CRMP4 impedes axon regeneration. Here, we investigated the involvement of CRMP4 in peripheral nerve injury in male and female Crmp4 À/À mice following sciatic nerve injury. We find that sensory axon regeneration and Wallerian degeneration are impaired in Crmp4 À/À mice following sciatic nerve injury. In vitro analysis of dissociated dorsal root ganglion (DRG) neurons from Crmp4 À/À mice revealed that CRMP4 functions in the proximal axon segment to promote the regrowth of severed DRG neurons and in the distal axon segment where it facilitates Wallerian degeneration through calpain-dependent formation of harmful CRMP4 fragments. These findings reveal an interesting dual role for CRMP4 in proximal and distal axon segments of injured sensory neurons that coordinately facilitate PNS axon regeneration.

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“…Western blot analysis was performed as previously described [41, 42]. Briefly, lysates were separated using SDS-PAGE and were electrophoretically transferred to a polyvinylidenedifluoride (PVDF) membrane.…”

Section: Methodsmentioning

confidence: 99%

“…Whole-cell patch-clamp recordings of miniature excitatory postsynaptic currents (mEPSC) were obtained from transfected cultured hippocampal neurons treated with A β oligomers on DIV 12-13 [41, 43]. During the recordings, cells were bathed in an external solution with a pH of 7.3, containing (in mM): 128 NaCl, 5 KCl, 2 CaCl 2 , 1 MgCl 2 , 15 glucose, 20 HEPES, 1 tetrodotoxin, and 100 μ M picrotoxin.…”

Section: Methodsmentioning

confidence: 99%

Endophilin2 Interacts with GluA1 to Mediate AMPA Receptor Endocytosis Induced by Oligomeric Amyloid-β

Zhang

Yin

et al. 2017

Neural Plasticity

Self Cite

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Amyloid-β (Aβ) plays an important role in Alzheimer's disease (AD), as oligomeric Aβ induces loss of postsynaptic AMPA receptors (AMPARs) leading to cognitive deficits. The loss of postsynaptic AMPARs is mediated through the clathrin-dependent endocytosis pathway, in which endophilin2 is one of the important regulatory proteins. Endophilin2, which is enriched in both the pre- and postsynaptic membrane, has previously been reported to be important for recycling of synaptic vesicles at the presynaptic membrane. However, the role of endophilin2 in oligomeric Aβ-induced postsynaptic AMPAR endocytosis is not well understood. In this study, we show that endophilin2 does not affect constitutive AMPAR endocytosis. Endophilin2 knockdown, but not overexpression, resisted oligomeric Aβ-induced AMPAR dysfunction. Moreover, endophilin2 colocalized and interacted with GluA1, a subunit of AMPAR, to regulate oligomeric Aβ-induced AMPAR endocytosis. Thus, we have determined a role of endophilin2 in oligomeric Aβ-induced postsynaptic AMPAR dysfunction, indicating possible directions for preventing the loss of AMPARs in cognitive impairment and providing evidence for the clinical treatment of AD.

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CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons

Cited by 22 publications

References 35 publications

Comprehensive behavioral study and proteomic analyses of CRMP2‐deficient mice

Comprehensive behavioral study and proteomic analyses of CRMP2‐deficient mice

Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury

Endophilin2 Interacts with GluA1 to Mediate AMPA Receptor Endocytosis Induced by Oligomeric Amyloid-β

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