Crocin treatment promotes the oxidative stress and apoptosis in human thyroid cancer cells FTC‐133 through the inhibition of STAT/JAK signaling pathway

Abstract

Thyroid cancer is the most frequent endocrine malignancy, which accounts for nearly 1% of all the cancer worldwide. Crocin has a diverse biological function, such as anti‐cancer, anti‐inflammatory and antioxidant functions, specifically in the respiratory related diseases. Using in vitro techniques, this work was intended to illuminate the anti‐cancer effect of crocin in follicular thyroid carcinoma (FTC) (FT 133 cells), and the potential molecular mechanism convoluted. The outcome of the present work showed that crocin was able to prevent the proliferation and triggering the apoptosis in a dose‐dependent mode, of FTC‐133 cells by methyl thiazolyldiphenyl‐tetrazolium bromide and staining assay (acridine orange/propiduim iodide [PI], 4′,6‐diamidino‐2‐phenylindole, and PI dye). Crocin did not show toxicity to the normal thyroid (PCCL3) cells. Crocin‐induced reactive oxygen species, mitochondrial membrane potential activity, caspase‐8 and ‐9, lipid peroxidation (thiobarbituric acid reactive substances) activity while suppressing antioxidant activity (superoxide dismutase, catalase, and glutathione) in FTC‐133 cells. In addition, crocin was also participated in a halting of the proteins related to cell cycle, cyclin D1, and pro‐apoptotic proteins; Bax and caspase‐3 expression, together with the elevation of anti‐apoptotic factor Bcl‐2. Further, crocin have a dual inhibition of two major pathways, nuclear factor‐κB, extracellular signal‐regulated kinase, and janus kinase‐signal transducer and activator of transcription signaling pathways. In conclusion, crocin can inhibit follicular thyroid carcinoma proliferation and promote cell apoptosis.

Propofol induces the ferroptosis of colorectal cancer cells by downregulating STAT3 expression

“…SW480 cells treated with propofol and/or transfected with pc-STAT3 were harvested and transferred to a centrifuge tube, then centrifuged for 10 min at 1,000 × g at room temperature. The process was conducted according to a previous study ( 19 ). The absorbance of the aforementioned solution was detected by a microplate reader at 535 nM.…”

The predictive value of prognosis and therapeutic response for STAT family in pancreatic cancer

“…Several researches have suggested the dysregulation of STATs is closely related to the initiation and progression of many tumors including hematologic malignancies and solid tumors [ [30] , [31] , [32] , [33] ]. STATs not only regulate cancer cell proliferation and differentiation, but also impact cell apoptosis [ [34] , [35] , [36] ]. Although the prognostic roles of STATs in certain cancers have been reported, there has been no research about differential expression and roles of STATs in PC.…”

An In Vitro Study of Saffron Carotenoids: The Effect of Crocin Extracts and Dimethylcrocetin on Cancer Cell Lines

“…Treatment of human normal fibroblastic cells (HFSF-PI3) with 1.9539–3.9078 mg/mL of CRCs or fibroblast mouse (L929) cells with 1–2 mg/mL of saffron ethanolic extract did not elicit any apoptogenic effect [ 11 , 96 ]. Similarly, CRCs in doses up to 0.088 mg/mL did not demonstrate any toxicity to the normal thyroid (PCCL3) cells [ 97 ]. CRCs in a dose of 1–4 mg/mL on breast epithelial MCF-10A cells did not affect the NF-κB-mediated inflammation and proliferation [ 98 ].…”