Crohn's Disease: Current Pathogenetic Paradigms

Duchmann, Rainer; Zeitz, Martin

doi:10.1016/b978-012491543-5/50076-0

Cited by 4 publications

(2 citation statements)

References 161 publications

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“…It is known that healthy individuals become tolerant to gut microbiotica and food while they remain capable of mounting specific responses to vaccines applied by the oral route (Mowat et al, ). On the contrary, an inability to become tolerant to gut flora has been associated with severe disease conditions such as Crohn's disease (Duchmann and Zeitz, ), as well as an abnormal response to food antigens provokes celiac disease (Lundin et al, ) and food allergies (Taylor and Hefle, ).…”

Section: Commentarymentioning

confidence: 99%

Fluorescence Microscopy and Flow Cytometric Analysis of Peyer's Patches and Intestinal Immune Cells

López

2007

CP Toxicology

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In recent years researchers have become more aware of the importance of the gut-associated immune system since it is in direct interaction with the entry site for virus, bacteria, and all type of food contaminants, including numerous toxins that can alter mucosal immunity. Peyer's patches (PP) are considered the inductive site for protein antigen presentation in the gut as well as the starting point for IgA B-cell differentiation. The IgA found in feces comes from IgA secreted by IgA lamina propria lymphocytes (LPL), and its presence is a sign of normal physiology, in that IgA plays a role in absorption and immune defense against gut-associated pathogens. Methods presented in this unit are intended to analyze PP and intestinal intraepithelial and LPL to determine whether the complexity of the mucosal-associated lymphoid tissue and its components have been altered by any form of external damage. The protocols explain how to isolate and culture isolated cells; how to stain and analyze; and also how to cryopreserve the gut.

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Section: Commentarymentioning

confidence: 99%

Fluorescence Microscopy and Flow Cytometric Analysis of Peyer's Patches and Intestinal Immune Cells

López

2007

CP Toxicology

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“…Crohn ' s disease (CD) and ulcerative colitis are the two phenotypic prototypes of inflammatory bowel disease (IBD). CD is characterized by granulomatous inflammation throughout the entire gastrointestinal tract with the terminal ileum mainly affected 1 whereas ulcerative colitis is characterized by chronic continuous inflammation in the colorectum without granuloma formation. 2 Alhough the etiology of IBD remains unclear, it is well established that genetic factors, environmental factors, and a dysregulated immune response toward commensal bacteria contribute to the pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Identification of the predominant antigenic epitopes in intestinal flora in IBD

et al. 2008

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The normal intestinal flora is required for the development of intestinal inflammation in animal models of inflammatory bowel disease (IBD). In humans, several studies indicated a potential association of Escherichia coli (E. coli) with IBD. In addition, we have shown that T-cell clones of IBD patients cross react toward different enteric bacterial species and thus likely respond to conserved bacterial antigens. Therefore, we hypothesized that highly conserved E. coli proteins might be a reasonable candidate to screen for abnormal T-cell responses in IBD. We used high-throughput techniques for cloning, expression, and purification under native conditions of a set of 271 conserved proteins of E. coli, of which 196 were used for whole blood stimulations to assess peripheral T helper (T(H))-cell responses. In addition, because of the association of an adherent-invasive E. coli with Crohn's disease (CD), we included 13 pathogenicity factors of E. coli in the study. We observed that pools of these conserved E. coli proteins less frequently induced interferon-gamma (IFNgamma) production in peripheral T(H) cells in patients with CD and ankylosing spondylitis (AS) compared with healthy controls. In addition, lower percentage of patients with CD and AS responded toward single proteins. The reason for the decreased frequency of an in vitro T(H)-cell IFNgamma response toward E. coli proteins in peripheral blood of CD and AS patients, e.g., increased suppression needs to be clarified.

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From Community Analysis to Prototype: Creating an Online Matchmaker for Inflammatory Bowel Disease Patients

Kaminski

2016

Boundaryless Hospital

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Crohn's Disease: Current Pathogenetic Paradigms

Cited by 4 publications

References 161 publications

Fluorescence Microscopy and Flow Cytometric Analysis of Peyer's Patches and Intestinal Immune Cells

Fluorescence Microscopy and Flow Cytometric Analysis of Peyer's Patches and Intestinal Immune Cells

Identification of the predominant antigenic epitopes in intestinal flora in IBD

From Community Analysis to Prototype: Creating an Online Matchmaker for Inflammatory Bowel Disease Patients

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