Cromoglycate mesogen forms isodesmic assemblies promoted by peptides and induces aggregation of a range of proteins

Ibanez, Arizza Chiara S.; Marji, Elaine; Luk, Yan Yeung

doi:10.1039/c8ra05226c

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…The DSCG-LCLC system has been utilized in many applications. 7,8,9 Notable among these are as an NMR aligning medium for the study of cyclodextrin host-guest complexes through residual quadrupolar splittings 10 and for structural studies of small molecules through the measurement of residual dipolar couplings (RDCs). 11,12,13,14 In addition, the nematic phase of DSCG has proven to be sensitive to the presence of small molecules (dopants).…”

Section: Introductionmentioning

confidence: 99%

Effect of the degree of protonation of amino acid dopants in Chirality amplification in DSCG Lyotropic Nematic phases.

Berride

Troche‐Pesqueira

Weiss

et al. 2021

Preprint

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Disodium Cromoglycate (DSCG), a lyotropic liquid crystal in water, is shown to be an amplifier of chirality when doped with small chiral molecules. Here, we study the behaviour of the DSCG nematic phase in the presence of three aminoacids with different degrees of protonation: L-Alanine, L-Arginine·HCl and L-Arginine. The results demonstrate that the sign of the helicity of the doped nematic phase (i.e., a cholesteric phase) depends on the sign of the Helical Twisting Power of the dopant.

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Section: Introductionmentioning

confidence: 99%

Effect of the degree of protonation of amino acid dopants in Chirality amplification in DSCG Lyotropic Nematic phases.

Berride

Troche‐Pesqueira

Weiss

et al. 2021

Preprint

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“…Intrinsic tryptophan fluorescence was used to demonstrate potential binding interactions between the pilin protein and our molecules. This fluorescence study was conducted with a recombinant version of the native pilin, a truncated version with the first 37 amino acids removed from the N′ terminus (Figure S4), previously made by our group [36] . The truncated version of pilin protein often preserves its ability to bind ligands while increasing the solubility [13a–c] …”

Section: Resultsmentioning

confidence: 99%

“…Cloning, recombinant expression and purification of truncated pilin protein : The expression and purification of truncated native pilin protein was adapted from protocols that were previously described [36] . Using the PureLink™ Quick Plasmid Miniprep Kit (Thermo Fisher Scientific), the pPAC46 plasmid containing the native pilA gene was extracted from PA1244N3(pPAC46).…”

Section: Methodsmentioning

confidence: 99%

Exploration of Ligand‐receptor Binding and Mechanisms for Alginate Reduction and Phenotype Reversion by Mucoid Pseudomonas aeruginosa

et al. 2021

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Bacteria in general can develop a wide range of phenotypes under different conditions and external stresses. The phenotypes that reside in biofilms, overproduce exopolymers, and show increased motility often exhibit drug tolerance and drug persistence. In this work, we describe a class of small molecules that delay and inhibit the overproduction of alginate by a non‐swarming mucoid Pseudomonas aeruginosa. Among these molecules, selected benzophenone‐derived alkyl disaccharides cause the mucoid bacteria to swarm on hydrated soft agar gel and revert the mucoid to a nonmucoid phenotype. The sessile (biofilm) and motile (swarming) phenotypes are controlled by opposing signaling pathways with high and low intracellular levels of bis‐(3’,5’)‐cyclic diguanosine monophosphate (cdG), respectively. As our molecules control several of these phenotypes, we explored a protein receptor, pilin of the pili appendages, that is consistent with controlling these bioactivities and signaling pathways. To test this binding hypothesis, we developed a bacterial motility‐enabled binding assay that uses the interfacial properties of hydrated gels and bacterial motility to conduct label‐free ligand‐receptor binding studies. The structure‐activity correlation and receptor identification reveal a plausible mechanism for reverting mucoid to nonmucoid phenotypes by binding pili appendages with ligands capable of sequestering and neutralizing reactive oxygen species.

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Properties of the uniaxial and biaxial nematic phases of the dodecyltrimethylammonium bromide/1-dodecanol/water lyotropic mixture including azo dye and drug molecules

Akpinar,

Tabak,

Reis

et al. 2023

Phase Transitions

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Cromoglycate mesogen forms isodesmic assemblies promoted by peptides and induces aggregation of a range of proteins

Abstract: 5′DSCG molecules form isodesmic assembly in the presence of peptides, and cause a wide range of proteins to aggregate.

Cited by 4 publications

References 53 publications

Effect of the degree of protonation of amino acid dopants in Chirality amplification in DSCG Lyotropic Nematic phases.

Effect of the degree of protonation of amino acid dopants in Chirality amplification in DSCG Lyotropic Nematic phases.

Exploration of Ligand‐receptor Binding and Mechanisms for Alginate Reduction and Phenotype Reversion by Mucoid Pseudomonas aeruginosa

Properties of the uniaxial and biaxial nematic phases of the dodecyltrimethylammonium bromide/1-dodecanol/water lyotropic mixture including azo dye and drug molecules

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