Cronobacter sakazakii Pyridoxal Kinase PdxY Mediated by TreR and pESA3 Is Essential for Vitamin B
            <sub>6</sub>
            (PLP) Maintenance and Virulence

Cronobacter sakazakii poses a significant threat, particularly to neonates and infants. Despite its strong pathogenicity, understanding of C. sakazakii biofilms and their role in infections remains limited. This study investigates the roles of HmsP and c-di-GMP in biofilm formation and identifies key genetic and proteomic elements involved. Gene knockout experiments reveal that HmsP and c-di-GMP are linked to biofilm formation in C. sakazakii . Comparative proteomic profiling identifies the lysozyme inhibitor protein LprI, which is downregulated in hmsP knockouts and upregulated in c-di-GMP knockouts, as a potential biofilm formation factor. Further investigation of the lprI knockout strain shows significantly reduced biofilm formation and decreased virulence in a rat infection model. Additionally, LprI is demonstrated to bind extracellular DNA, suggesting a role in anchoring C. sakazakii within the biofilm matrix. These findings enhance our understanding of the molecular mechanisms underlying biofilm formation and virulence in C. sakazakii , offering potential targets for therapeutic intervention and food production settings. IMPORTANCE Cronobacter sakazakii is a bacterium that poses a severe threat to neonates and infants. This research elucidates the role of the lysozyme inhibitor LprI, modulated by HmsP and c-di-GMP, and uncovers a key factor in biofilm formation and virulence. The findings offer crucial insights into the molecular interactions that enable C. sakazakii to form resilient biofilms and persist in hostile environments, such as those found in food production facilities. These insights not only enhance our understanding of C. sakazakii pathogenesis but also identify potential targets for novel therapeutic interventions to prevent or mitigate infections. This work is particularly relevant to public health and the food industry, where controlling C. sakazakii contamination in powdered infant formula is vital for safeguarding vulnerable populations.

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Impact of DnaK on protein expression and deamidation modification in Cronobacter sakazakii : insights from proteomic analysis

Lu,

Xue,

Chen

et al. 2023

Microbiol Resour Announc

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Cronobacter sakazakii Pyridoxal Kinase PdxY Mediated by TreR and pESA3 Is Essential for Vitamin B ₆ (PLP) Maintenance and Virulence

Cited by 3 publications

References 54 publications

Controlling Cronobacter spp. in dairy manufacturing – Fundamental characteristics and practical guidance

Controlling Cronobacter spp. in dairy manufacturing – Fundamental characteristics and practical guidance

Cronobacter sakazakii lysozyme inhibitor LprI mediated by HmsP and c-di-GMP is essential for biofilm formation and virulence

Impact of DnaK on protein expression and deamidation modification in Cronobacter sakazakii : insights from proteomic analysis

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Cronobacter sakazakii Pyridoxal Kinase PdxY Mediated by TreR and pESA3 Is Essential for Vitamin B 6 (PLP) Maintenance and Virulence

Cited by 3 publications

References 54 publications

Controlling Cronobacter spp. in dairy manufacturing – Fundamental characteristics and practical guidance

Controlling Cronobacter spp. in dairy manufacturing – Fundamental characteristics and practical guidance

Cronobacter sakazakii lysozyme inhibitor LprI mediated by HmsP and c-di-GMP is essential for biofilm formation and virulence

Impact of DnaK on protein expression and deamidation modification in Cronobacter sakazakii : insights from proteomic analysis

Contact Info

Product

Resources

About

Cronobacter sakazakii Pyridoxal Kinase PdxY Mediated by TreR and pESA3 Is Essential for Vitamin B ₆ (PLP) Maintenance and Virulence