Polymer-coated urea (PCU) is used in potato (Solanum tuberosum L.) production to maintain or improve tuber yield while minimizing N losses, but the mode of N release is different from conventional soluble N sources. Hence, it is not known how soil-and plant-based indices of N availability would perform in response to PCU application. A 3-yr study was conducted on a sandy-loam soil in Quebec, Canada, to examine the effect of PCU application rate (0, 60, 120, 200, and 280 kg N ha -1 ) on petiole NO 3 -N concentrations, chlorophyll meter readings (SPAD readings), soil mineral N content, and total tuber yield. Nitrate availability throughout the growing season was monitored by measuring NO 3 adsorbed by anion exchange membranes (NO 3AEM ). The NO 3AEM values, petiole NO 3 -N concentrations, SPAD readings, soil mineral N content, and total tuber yield increased with PCU application rate. The NO 3AEM values fluctuated during the growing season due to plant N uptake and variations in soil moisture content. Using all the PCU rates across years, we found that relative yield was better related to petiole NO 3 -N concentrations measured at 45 to 50 d after planting (DAP) (R 2 = 0.95) and to cumulative NO 3AEM values measured up to 49 DAP (R 2 = 0.91) than soil mineral N (R 2 = 0.67), soil NO 3 -N (R 2 = 0.65), and SPAD readings (R 2 = 0.53) measured at 45 to 50 DAP, respectively. This study showed that soil-and plant-based indices of N availability can be used as early indicators of yield response in potato fertilized with PCU.