As one of the staple food crops,rice (Oryza sativa L.) is widely cultivated across China, which plays a critical role in guaranteeing national food security. Most previous studies on grain yield or/and nitrogen use efficiency (NUE) of rice in China often involved site-specific field experiments, or small regions with insufficient data, which limited the representation for the current rice production regions. In this study, a database covering a wide range of climate conditions, soil types and field managements across China, was developed to estimate rice grain yield and NUE in various rice production regions in China and to evaluate the relationships between N rates and grain yield, NUE. According to the database for rice, the values of grain yield, plant N accumulation, N harvest index (HI N), Indigenous N supply (INS), internal N efficiency (IE N), reciprocal internal N efficiency (RIE N), agronomic N use efficiency (AE N), partial N factor productivity (PEP N), physiological N efficiency (PE N) and recover efficiency of applied N (RE N) averaged 7.