Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

Jolivet, Pascale; Acevedo, Francisca; Boulard, Céline; d’Andréa, Sabine; Faure, Jean-Denis; Kohli, Ajay; Nési, Nathalie; Valot, Benoı̂t; Chardot, Thierry

doi:10.1002/pmic.201200431

Cited by 46 publications

(50 citation statements)

References 93 publications

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“…6A). In a typical LD topology, neutral lipids are covered by a polar membrane shell (Jolivet et al, 2013). Since smaller LDs contain a higher proportion of polar membrane lipids than larger LDs, this suggests that the LDs in SL-exposed cells are smaller than those induced by N starvation, which was consistent with microscopic examinations (Fig.…”

Section: Lds In Sl-exposed Cells Contain Tags and Polar Lipids Of Mossupporting

confidence: 78%

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Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii

et al. 2016

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Enriching algal biomass in energy density is an important goal in algal biotechnology. Nitrogen (N) starvation is considered the most potent trigger of oil accumulation in microalgae and has been thoroughly investigated. However, N starvation causes the slow down and eventually the arrest of biomass growth. In this study, we show that exposing a Chlamydomonas reinhardtii culture to saturating light (SL) under a nonlimiting CO 2 concentration in turbidostatic photobioreactors induces a sustained accumulation of lipid droplets (LDs) without compromising growth, which results in much higher oil productivity than N starvation. We also show that the polar membrane lipid fraction of SL-induced LDs is rich in plastidial lipids (approximately 70%), in contrast to N starvation-induced LDs, which contain approximately 60% lipids of endoplasmic reticulum origin. Proteomic analysis of LDs isolated from SL-exposed cells identified more than 200 proteins, including known proteins of lipid metabolism, as well as 74 proteins uniquely present in SL-induced LDs. LDs induced by SL and N depletion thus differ in protein and lipid contents. Taken together, lipidomic and proteomic data thus show that a large part of the sustained oil accumulation occurring under SL is likely due to the formation of plastidial LDs. We discuss our data in relation to the different metabolic routes used by microalgae to accumulate oil reserves depending on cultivation conditions. Finally, we propose a model in which oil accumulation is governed by an imbalance between photosynthesis and growth, which can be achieved by impairing growth or by boosting photosynthetic carbon fixation, with the latter resulting in higher oil productivity.

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Section: Lds In Sl-exposed Cells Contain Tags and Polar Lipids Of Mossupporting

confidence: 78%

“…Oil accumulation is associated with the formation of lipophilic droplets, called lipid droplets (LDs [or oil bodies or oleosomes]; Jolivet et al, 2013). LDs are specialized intracellular organelles made of a neutral lipid core surrounded by a membrane lipid coat in which proteins are embedded (Huang, 1996).…”

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Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii

et al. 2016

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“…To understand the diverse functions of lipid droplets, an array of proteins have been identified that are associated with lipid droplets from different cell types and organisms (Cermelli et al, 2006;Hodges and Wu, 2010;Yang et al, 2012;Jolivet et al, 2013). In higher plants, oleosins, caleosins, steroleosins, and other seedspecific lipid droplet proteins have been widely recognized for their role in lipid compartmentalization in oilseeds and some floral tissues, such as anther and pollen (Huang, 1992;Tzen and Huang, 1992;Frandsen et al, 2001;Lin et al, 2005;Huang et al, 2013).…”

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Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

et al. 2013

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Lipid droplets in plants (also known as oil bodies, lipid bodies, or oleosomes) are well characterized in seeds, and oleosins, the major proteins associated with their surface, were shown to be important for stabilizing lipid droplets during seed desiccation and rehydration. However, lipid droplets occur in essentially all plant cell types, many of which may not require oleosinmediated stabilization. The proteins associated with the surface of nonseed lipid droplets, which are likely to influence the formation, stability, and turnover of this compartment, remain to be elucidated. Here, we have combined lipidomic, proteomic, and transcriptomic studies of avocado (Persea americana) mesocarp to identify two new lipid droplet-associated proteins, which we named LDAP1 and LDAP2. These proteins are highly similar to each other and also to the small rubber particle proteins that accumulate in rubber-producing plants. An Arabidopsis (Arabidopsis thaliana) homolog to LDAP1 and LDAP2, At3g05500, was localized to the surface of lipid droplets after transient expression in tobacco (Nicotiana tabacum) cells that were induced to accumulate triacylglycerols. We propose that small rubber particle protein-like proteins are involved in the general process of binding and perhaps the stabilization of lipid-rich particles in the cytosol of plant cells and that the avocado and Arabidopsis protein members reveal a new aspect of the cellular machinery that is involved in the packaging of triacylglycerols in plant tissues.

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“…Drying management may brought about over expression of DGAT (Weselake et al 2008). Oil bodies are the main form of oil in mature seed, internally containing liquid TAG and externally forming phospholipid monolayer (Jolivet et al 2013). We identified the presence of oleosins and caleosins proteins in camellia seed, and involved in 4 oleosin unigenes (CL734Contig1, CL422Contig1, CL32127Contig1 and CL25464Contig1) with expression over 1000 RPKM in average (Table 7, S4).…”

Section: Discussion Fatty Acids Biosynthesis and Accumulationmentioning

confidence: 99%

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

Feng

Yang

Weiwei

et al. 2017

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Key message The molecular mechanisms of fatty acid biosynthesis and accumulation during Camellia meiocarpa and Camellia oleifera seed natural drying are characterized through transcriptome analyses. Abstract Camellia seed oil has been used as high quality and healthy food for over two thousand years. Seed drying affects oil quality and quantity; however, the molecular mechanisms of fatty acid biosynthesis and accumulation during the drying process remain unknown. In this study, the transcriptomes of Camellia meiocarpa and C. oleifera seed were characterized at five moisture content levels (10-50%) to identify the major processes and reveal genes affecting lipid metabolism in response to natural drying. We found a total of 111,156 unigenes by de novo assembled from RNA-Seq libraries of five moisture content levels during after-ripening of C. meiocarpa (74,016) and C. oleifera (76,374). Ten pathways were closely linked to changes in oil content and composition with 244 genes involved in fatty acid synthesis and accumulation. Gene ontology enrichment of differentially expressed genes (DEGs) indicated that fatty acid synthesis and accumulation are essential in C. meiocarpa while fatty acid accumulation in C. oleifera during natural drying process. Comparative analyses of DEGs between any two consecutive moisture contents identified six and three key unigenes in C. meiocarpa and C. oleifera seeds, respectively, and one additional unigene responsible for the difference between the two species' fatty acid synthesis and accumulation. Natural drying has improved the quality and quantity of the camellia seed oil. The study provided: (a) global transcriptional profiles at five moisture content levels during seed natural drying, (b) highlighting transcripts putatively involved in the regulation of gene expression program and in specific processes likely essential for lipid metabolism, and (c) discovery of genes associated with oil seed quantity and quality improvement for the studied two camellia species.Communicated by M. Buckeridge. Electronic supplementary materialThe online version of this article

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Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

Cited by 46 publications

References 93 publications

Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii

Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii

Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

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