Cross amplification of 15 EST-SSR markers in the genus Fraxinus

Noakes, Andrea G.; Best, Teodora; Stanton, Margaret E.; Koch, Jennifer; Romero‐Severson, Jeanne

doi:10.1007/s12686-014-0260-2

Cited by 11 publications

(14 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…excelsior [ 60 ]. ASH2429 [ 61 ], FRESTSSR279, FRESTSSR308, FRESTSSR427, FRESTSSR528 [ 62 ] and Fp12378, Fp18437, Fp20456, Fp21064, Fp104136 [ 63 ] are EST-SSRs developed for F . excelsior or other Fraxinus species.…”

Section: Methodsmentioning

confidence: 99%

Gene flow of common ash (Fraxinus excelsior L.) in a fragmented landscape

2017

View full text Add to dashboard Cite

Gene flow dynamics of common ash (Fraxinus excelsior L.) is affected by several human activities in Central Europe, including habitat fragmentation, agroforestry expansion, controlled and uncontrolled transfer of reproductive material, and a recently introduced emerging infectious disease, ash dieback, caused by Hymenoscyphus fraxineus. Habitat fragmentation may alter genetic connectivity and effective population size, leading to loss of genetic diversity and increased inbreeding in ash populations. Gene flow from cultivated trees in landscapes close to their native counterparts may also influence the adaptability of future generations. The devastating effects of ash dieback have already been observed in both natural and managed populations in continental Europe. However, potential long-term effects of genetic bottlenecks depend on gene flow across fragmented landscapes. For this reason, we studied the genetic connectivity of ash trees in an isolated forest patch of a fragmented landscape in Rösenbeck, Germany. We applied two approaches to parentage analysis to estimate gene flow patterns at the study site. We specifically investigated the presence of background pollination at the landscape level and the degree of genetic isolation between native and cultivated trees. Local meteorological data was utilized to understand the effect of wind on the pollen and seed dispersal patterns. Gender information of the adult trees was considered for calculating the dispersal distances. We found that the majority of the studied seeds (55–64%) and seedlings (75–98%) in the forest patch were fathered and mothered by the trees within the same patch. However, we determined a considerable amount of pollen flow (26–45%) from outside of the study site, representing background pollination at the landscape level. Limited pollen flow was observed from neighbouring cultivated trees (2%). Both pollen and seeds were dispersed in all directions in accordance with the local wind directions. Whereas there was no positive correlation between pollen dispersal distance and wind speed, the correlation between seed dispersal distance and wind speed was significant (0.71, p < 0.001), indicating that strong wind favours long-distance dispersal of ash seeds. Finally, we discussed the implications of establishing gene conservation stands and the use of enrichment planting in the face of ash dieback.

show abstract

Section: Methodsmentioning

confidence: 99%

Gene flow of common ash (Fraxinus excelsior L.) in a fragmented landscape

2017

View full text Add to dashboard Cite

show abstract

“…The ash stands in the forest are of different origin and age (Semizer-Cuming et al 2020). The two stands in the west (1.0 ha) and in the north east (0.4 ha) include ash trees planted in 1958 based on seeds from an old-Danish seed orchard ('FP202' consisting of 8 clones) (Nielsen et al 2009). Most other mature ash trees in the surveyed area are from 1934 to 1956 and of putative Danish origin, either from natural seeding or plantings with seeds procured from other Danish ash forests.…”

Section: Study Site and Sampling Designmentioning

confidence: 99%

Gene flow and reproductive success in ash (Fraxinus excelsior L.) in the face of ash dieback: restoration and conservation

Semizer-Cuming

Chybicki

Finkeldey

et al. 2021

Annals of Forest Science

View full text Add to dashboard Cite

• Key message Ash dieback decreases individual reproductive success of ash trees leaving healthy ash overrepresented as seed and pollen parents for next-generation seedlings. Substantial gene flow over hundreds of meters combined with superior fertility of healthy trees creates optimism for the species’ future in European forests. • Context Hymenoscyphus fraxineus is causing high mortality in European ash (Fraxinus excelsior L.). Due to inheritable resistance to the pathogen, natural selection is likely to act in favour of improved resistance in ash forests following natural regeneration. Still, the frequency of healthy trees is low, and the effect of natural selection will depend on survival, reproductive success and the dispersal capacity of healthy trees under natural conditions. • Aims We aim to test whether healthy trees contribute more to the next generation and to infer their potential for dispersing progenies across the forested landscape. • Methods Using parentage modelling, we estimate mating parameters and dispersal distances of seeds and pollen and compare realised reproductive success of healthy trees to that of unhealthy ones. • Results Healthy trees are overrepresented as the parents of randomly sampled seeds and seedlings in the forest, although that is more pronounced on the female side. We observe long dispersal events and estimate the mean seed and pollen dispersal distances as 67 m and 347 m, respectively. • Conclusion Variation in reproductive success results in selection in favour of lowered susceptibility to ash dieback. The large dispersal capacity decreases the risk of genetic bottlenecks and inbreeding and allows resistant trees to disperse their genes into the neighbourhoods of substantial sizes.

show abstract

“…69181) according to the manufacturer's protocol (Qiagen, Hilden, Germany). Microsatellite analyses were performed with nine selected primer pairs: FEMSATL11 and FEMSATL19 (Lefort et al 1999); FEMSATL12 (Gerard et al 2006); ASH2429 (Bai et al 2011); FRESTSSR308, FRESTSSR427, and FRESTSSR528 (Aggarwal et al 2011); and Fp18437 and Fp21064 (Noakes et al 2014). The primers were labelled with four different fluorescent dyes (6-FAM: FEMSATL12, Fp18437, and Fp21064; VIC: FEMSATL11, FRESTSSR308, and FRESTSSR528; NED: FEMSATL19 and ASH2429; PET: FRESTSSR427) and combined in three multiplexes (Multiplex-1: FEMSATL11, FEMSATL12 and FEMSATL19; Multiplex-2: Fp18437, Fp21064, and F R E S T S S R 5 2 8 ; M u l t i p l e x -3 : F R E S T S S R 3 0 8 , FRESTSSR427, and ASH2429).…”

Section: Dna Genotypingmentioning

confidence: 99%

Negative correlation between ash dieback susceptibility and reproductive success: good news for European ash forests

Semizer-Cuming

Finkeldey

Nielsen

et al. 2019

Annals of Forest Science

View full text Add to dashboard Cite

& Key message European ash (Fraxinus excelsior L.) trees with low susceptibility to ash dieback have higher reproductive fitness compared to highly susceptible trees, although most pronounced for female success. Selection at generation turnover therefore supports the future recovery of ash forests. & Context The introduced invasive pathogen Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, and Hosoya cause extensive damage on European ash (Fraxinus excelsior L.). Heritable variation in susceptibility to ash dieback has been observed among ash trees in natural and planted populations, but it is not clear how variation in susceptibility influences reproductive fitness. & Aims We hypothesize that healthier male and female trees contribute more gametes to the following generation compared to unhealthy ones. & Methods We tested the hypothesis by studying gender, seed production, and paternal success in a clonal field trial with 39 replicated clones. In the trial, the susceptibility level of each clone has been recorded in terms of percent crown damage since 2007. We used a linear regression model to explore the relationship between susceptibility and reproductive success (female and male). & Results The clones revealed a clear gender dimorphism with an approximate 2:2:1 male/female/hermaphrodite ratio. Females with low levels of crown damage produced substantially more seeds compared to highly damaged females. The male clone with the lowest level of susceptibility was the most effective pollen donor, but highly susceptible males also sired some offspring. & Conclusion The results overall represent good news for the potential recovery of ash forests: selection against most susceptible genotypes at generation turnover is expected to facilitate building up disease resistance in ash populations.

show abstract

Cross amplification of 15 EST-SSR markers in the genus Fraxinus

Cited by 11 publications

References 5 publications

Gene flow of common ash (Fraxinus excelsior L.) in a fragmented landscape

Gene flow of common ash (Fraxinus excelsior L.) in a fragmented landscape

Gene flow and reproductive success in ash (Fraxinus excelsior L.) in the face of ash dieback: restoration and conservation

Negative correlation between ash dieback susceptibility and reproductive success: good news for European ash forests

Contact Info

Product

Resources

About