Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer

Abstract

ABSTRACT. Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully in noninvasive samples from these species. We also compared these nonspecific loci against eight polymorphic loci that were recently developed for huemul to determine whether the nonspecific markers could reflect the huemul's genetic variation that was observed with the specific loci. We identified 10 suitable loci, six of which constitute a standardized set for the two species and can be used to identify them in the absence of phenotypic data. The expected heterozygosity per locus for the panel of six loci ranged from 0.461 to 0.889 (average 0.665), and the maximum probability of identity value was 6.9 x 10 -6 and 3.2 x 10 -4 in pudu and huemul, respectively. This set of loci has potential applications in evolutionary, ecological, forensic, and conservation studies in pudu and huemul.

Paternity Determination of Captive Southern Pudu (<i>Pudu puda</i>) Using Bovine and Ovine Microsatellite Markers

“…Ryo TADANO 1) ＊ , Satoshi KUSUDA 1) and Hideto TOYODA 2) 1) Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan ≥ 0.500), although these values were estimated based on the genotypes of only six individuals. In a previous study [10], two bovine markers (BM203 and BM6506) showed relatively high polymorphism in southern pudu. Similar trends for these two markers were observed in the current study.…”

Transferibilidad de marcadores microsatélites de Anas platyrhynchos al pato criollo peruano Cairina moschata domestica

“…En resumen, los SSR exitosamente transferibles y estandarizados en eficientes protocolos de PCR que se muestran en nuestro trabajo permitirán estudiar las poblaciones de patos criollos evaluando factores como su diversidad genética, estructura genética poblacional, flujo génico, evolución, tasa de endogamia, entre otros aspectos de importancia para la conservación de la especie, así como para los programas de mejoramiento genético de la misma (Shafer et al 2012, Orozco-Terwengel 2013, Marín et al 2014.…”