Cross-amplified microsatellites in the European cherry fly,<i>Rhagoletis cerasi</i>: medium polymorphic–highly informative markers

Augustinos, Antonios A.; Asimakopoulou, Anastasia K.; Papadopoulos, Nikos T.; Bourtzis, Kostas

doi:10.1017/s0007485310000167

Cited by 12 publications

(16 citation statements)

References 49 publications

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“…To separate environmental from genetic factors contributing to the potential variation in diapause intensity among the three populations, their genetic structure should be considered. Using microsatellite markers, it has recently been revealed that the two Greek populations used in our study form one cluster with moderate levels of gene flow, whereas the German population is quite different from the previous cluster (Augustinos et al ., , ).…”

Section: Methodsmentioning

confidence: 99%

Diapause termination of Rhagoletis cerasi pupae is regulated by local adaptation and phenotypic plasticity: escape in time through bet‐hedging strategies

Moraiti

Nakas

Papadopoulos

2013

J of Evolutionary Biology

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Persistence and thriving of univoltine, herbivore insect species of the temperate zone rely on obligate diapause response that ensures winter survival and synchronization with host phenology. We used a stenophagous fruit fly (Rhagoletis cerasi) with obligate pupae diapause to determine genetic and environmental effects on diapause intensity of geographically isolated populations with habitat heterogeneity. Pupae from two Greek and one German populations with various gene flow rates were exposed at five constant chilling temperatures (0-12°C) for different durations and then incubated at a high temperature until all adults have emerged. Pupae diapause intensity differs among Greek and German populations, suggesting an adaptive response to habitat heterogeneity (mostly differences in phenology patterns of local host cultivars). Moderately warm winter temperatures, such as 8°C, promote diapause termination in all three populations. Insufficient chilling (short duration or warmer temperatures) regulates the expression of prolonged dormancy. Interestingly, extended chilling (longer than required for terminating diapause) 'return' pupae to another (facultative) cycle of dormancy enabling adults to emerge during the next appropriate 'window of time'; a strategy first time reported for univoltine insects. Consequently, diapause duration of R. cerasi is determined both by i) the adaptive response to local climatic conditions (annual dormancy) and ii) the plastic responses to interannual climatic variability resulting in two types of long life cycles within populations, prolonged and facultative dormancy as response to insufficient chilling and extended exposure to chilling, respectively. Long life cycles are expressed as a part of dormancy bet-hedging strategies of R. cerasi populations.

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Section: Methodsmentioning

confidence: 99%

Diapause termination of Rhagoletis cerasi pupae is regulated by local adaptation and phenotypic plasticity: escape in time through bet‐hedging strategies

Moraiti

Nakas

Papadopoulos

2013

J of Evolutionary Biology

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“…Microsatellites are a class of highly polymorphic molecular markers widely distributed in the genome of eukaryotes that can be useful to clarify such patterns of gene flow and to identify the spatial locations of genetic discontinuities (population boundaries) in studies of species complexes (Chambers and MacAvoy 2000, Barbará et al 2007, Aketarawong et al 2014). Within the Tephritidae, microsatellites have been successfully developed and applied for several Bactrocera species (Shearman et al 2006; Aketarawong et al 2006, 2007, Augustinos et al, 2008, Virgilio et al 2010; Drew et al 2011), for Rhagoletis cerasi L. (Augustinos et al 2011), for a few Ceratitis species (Bonizzoni et al 2001, 2004, Meixner et al 2002, Baliraine et al 2003, 2004, Silva et al 2003, Delatte et al 2013, Virgilio et al 2013), for Anastrepha suspensa (Loew) (Fritz and Schable 2004, Boykin et al 2010), and for Anastrepha obliqua (Macquart) (Islam et al 2011). Microsatellites have only recently been isolated in Anastrepha fraterculus (Lanzavecchia et al 2014) and have proven useful for the analysis of population dynamics and differentiation across the distribution range of this polymorphic species.…”

Section: Introductionmentioning

confidence: 99%

Relevant genetic differentiation among Brazilian populations of Anastrepha fraterculus (Diptera, Tephritidae)

Manni¹,

Lima²,

Guglielmino³

et al. 2015

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We used a population genetic approach to detect the presence of genetic diversity among six populations of Anastrepha fraterculus across Brazil. To this aim, we used Simple Sequence Repeat (SSR) markers, which may capture the presence of differentiative processes across the genome in distinct populations. Spatial analyses of molecular variance were used to identify groups of populations that are both genetically and geographically homogeneous while also being maximally differentiated from each other. The spatial analysis of genetic diversity indicates that the levels of diversity among the six populations vary significantly on an eco-geographical basis. Particularly, altitude seems to represent a differentiating adaptation, as the main genetic differentiation is detected between the two populations present at higher altitudes and the other four populations at sea level. The data, together with the outcomes from different cluster analyses, identify a genetic diversity pattern that overlaps with the distribution of the known morphotypes in the Brazilian area.

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“…Studies using microsatellites have been mainly focused on their use as polymorphic markers in population genetics, genetic diversity, or kinship contexts (reviewed in Schlötterer and Pemberton 1998;Goldstein and Schlötterer 1999). This is particularly true in insects, where reports based on SSR molecular variation are abundant (Insuan et al 2007;Agustinos et al 2011;Blondin et al 2013;Manrique-Poyato et al 2013). In recent years, microsatellite genomic analysis has been powered by the development of high throughput next generation sequencing (NGS) technologies, based on massive sequencing approaches which enable a rapid, low-cost, and low time-consuming way to characterize microsatellites (Malausa et al 2011;Iquebal et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Next generation sequencing and FISH reveal uneven and nonrandom microsatellite distribution in two grasshopper genomes

et al. 2014

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Simple sequence repeats (SSRs), also known as microsatellites, are one of the prominent DNA sequences shaping the repeated fraction of eukaryotic genomes. In spite of their profuse use as molecular markers for a variety of genetic and evolutionary studies, their genomic location, distribution, and function are not yet well understood. Here we report the first thorough joint analysis of microsatellite motifs at both genomic and chromosomal levels in animal species, by a combination of 454 sequencing and fluorescent in situ hybridization (FISH) techniques performed on two grasshopper species. The in silico analysis of the 454 reads suggested that microsatellite expansion is not driving size increase of these genomes, as SSR abundance was higher in the species showing the smallest genome. However, the two species showed the same uneven and nonrandom location of SSRs, with clear predominance of dinucleotide motifs and association with several types of repetitive elements, mostly histone gene spacers, ribosomal DNA intergenic spacers (IGS), and transposable elements (TEs). The FISH analysis showed a dispersed chromosome distribution of microsatellite motifs in euchromatic regions, in coincidence with chromosome location patterns previously observed for many mobile elements in these species. However, some SSR motifs were clustered, especially those located in the histone gene cluster.

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Cross-amplified microsatellites in the European cherry fly,Rhagoletis cerasi: medium polymorphic–highly informative markers

Cited by 12 publications

References 49 publications

Diapause termination of Rhagoletis cerasi pupae is regulated by local adaptation and phenotypic plasticity: escape in time through bet‐hedging strategies

Diapause termination of Rhagoletis cerasi pupae is regulated by local adaptation and phenotypic plasticity: escape in time through bet‐hedging strategies

Relevant genetic differentiation among Brazilian populations of Anastrepha fraterculus (Diptera, Tephritidae)

Next generation sequencing and FISH reveal uneven and nonrandom microsatellite distribution in two grasshopper genomes

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