Cross‐contamination of the human salivary gland <scp>HSG</scp> cell line with HeLa cells: A <scp>STR</scp> analysis study

Lin, Li-Chieh; Elkashty, Osama A.; Ramamoorthi, Murali; Trinh, Nathalie; Liu, Younan; Sunavala‐Dossabhoy, Gulshan; Pranzatelli, Thomas; Michael, Drew G.; Chivasso, Clara; Perret, Jason; Chiorini, John A.; Delporte, Christine

doi:10.1111/odi.12920

Cited by 25 publications

(26 citation statements)

References 38 publications

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“…As with other tissues, monolayer culture methods and cell lines exist for the salivary glands, and have been used with varying degrees of success. However, cell lines have significant limitations, including poor recapitulation of native gland phenotype and function (Capes-Davis et al, 2010; Lin et al, 2018; Liu, Liao, & Ambudkar, 2001; Nelson, Manzella, & Baker, 2013). …”

Section: Introductionmentioning

confidence: 99%

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Varghese

Hansen

Sharipol

et al. 2019

Archives of Oral Biology

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Objective: The objective of this study was to characterize the mechanism by which salivary gland cells (SGC) aggregate in vitro. Design: Timelapse microscopy was utilized to analyze the process of salivary gland aggregate formation using both primary murine and human salivary gland cells. The role of cell density, proliferation, extracellular calcium, and secretory acinar cells in aggregate formation was investigated. Finally, the ability of cells isolated from irradiated glands to form aggregates was also evaluated. Results: Salivary gland cell self-organization rather than proliferation was the predominant mechanism of aggregate formation in both primary mouse and human salivary gland cultures (SGC). Aggregation was found to require extracellular calcium while acinar lineage cells account for ~80% of the total aggregate cell population. Finally, aggregation was not impaired by irradiation. Conclusions: The data reveal that aggregation occurs as a result of heterogeneous salivary gland cell self-organization rather than from stem cell proliferation and differentiation, contradicting previous dogma. These results suggest a re-evaluation of aggregate formation as a criterion defining salivary gland stem cells.

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Section: Introductionmentioning

confidence: 99%

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Varghese

Hansen

Sharipol

et al. 2019

Archives of Oral Biology

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“…Therefore, we initially decided to study each clade of LEG1 genes in the liver or salivary gland epithelial cells. However, no authenticated immortalized salivary gland epithelial cell lines exist at present, as previously established cell lines have been reported to be contaminated by other cells or are not well characterized or widely used [24][25][26][27]. Therefore, only the HepG2 liver cell line was employed in our current study.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Prediction of Pig Liver-Enriched Gene 1 Functions in a Liver Cell Line

Zhang

Wang

Dang

et al. 2020

Genes

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The newly identified liver-enriched gene 1 (LEG1) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that LEG1 genes are involved in liver development, while the platypus LEG1 homolog, Monotreme Lactation Protein (MLP), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian LEG1s have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian LEG1s can be classified into three paralogous groups. Pigs have all three LEG1 genes (pLEG1s), while humans and mice have retained only LEG1a. Hence, pLEG1s might represent an ideal model for studying LEG1 gene functions. RNA-seq was performed by the overexpression of pLEG1s and platypus MLP in HepG2 cells. Enrichment analysis showed that pLEG1a and pLEG1b might exhibit little function in liver cells; however, pLEG1c is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus MLP shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian LEG1s have different functions in liver cells due to the subfunctionalization of paralogous genes.

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“…The HeLa tumor-cell line, a human cervical cancer cell originating from an American woman Henrietta Lacks, was established in 1951. 20 This cell line has been widely used in cytotoxicity, tumor research and biological experiments in the medical field. [21][22][23] The morphology of the MSNRs were verified by field emission scanning electron microscope ( FESEM ) and transmission electron microscopy (TEM).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity induced by new spiral mesoporous silica nanorods via specific surface area and ROS accumulation in HeLa cells

She

Sun

et al. 2020

Mater. Adv.

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Cross‐contamination of the human salivary gland HSG cell line with HeLa cells: A STR analysis study

Abstract: Short tandem repeat analysis revealed that HSG was contaminated by the HeLa cell line. Furthermore, because genotyping of the original HSG cell line was not performed during its establishment, it will be difficult to authenticate an uncontaminated sample of HSG.

Cited by 25 publications

References 38 publications

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Transcriptomic Prediction of Pig Liver-Enriched Gene 1 Functions in a Liver Cell Line

Cytotoxicity induced by new spiral mesoporous silica nanorods via specific surface area and ROS accumulation in HeLa cells

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