An ethnobiological study concerning the medical ethnobotany and ethnozoology of two neighbouring communities of Serbians and Albanians living in the Pešter plateau (south-western Serbia) was conducted, the latter representing a diasporic community that immigrated to the area approximately three centuries ago. Sixty-two botanical taxa used in 129 plant-based remedies and 204 folk plant uses were recorded. In addition, 31 animal-derived remedies and 27 mineral or non-indigenous products were also documented. Approximately half of the recorded phytotherepeutical uses have been recorded for the first time in the ethnobotany of the Western Balkans and more than onethird of these uses have no correlation with Western evidence-based phytotherapy. Moreover, while both communities use approximately the same number of medicinal plants, two-thirds of the botanical taxa, but only one-third of plant folk medical uses are found in common among the two communities. These findings demonstrate that the two communities, although having lived in close proximity to each other during the past three centuries and in a relatively low biodiverse environment, have maintained or developed unique phytotherapeutical trajectories. The differences between the two folk medical biologies of these communities are reflective of the specific history of the Albanian diaspora, and of the complex processes of its cultural adaptation over the last three centuries.