Cross-effects of nickel contamination and parasitism on zebra mussel physiology

Minguez, Laëtitia; Boiché, Anatole; Sroda, Sophie; Mastitsky, Sergey E.; Brulé, Nelly; Bouquerel, Jonathan; Giambérini, Laure

doi:10.1007/s10646-011-0814-y

Cited by 14 publications

(6 citation statements)

References 50 publications

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“…Hemocyte viability, phagocytosis, ROS production, lysosomal system size and CSP-3 induction were measured by flow-cytometry (FACScaliburTM, BD Biosciences) using protocols adapted from Minguez et al (2012).…”

Section: Biomarker Measurements -Detailed Protocols In Smmentioning

confidence: 99%

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

et al. 2016

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Exposures in realistic environmental conditions are essential to properly assess the effects of emerging pollutants on ecosystems. While ceria nanoparticles (nCeO2) production and use are expanding quickly, ecotoxicity studies remain very scarce. In this study, we set up experimental systems reproducing a simplified ecosystem to assess the effects of a chronic exposure to citrate-coated nCeO2 (ci-CeO2) and bare nCeO2 (ba-CeO2) on the freshwater mussel Dreissena polymorpha using an integrated multibiomarker approach. The fate of nanoparticles was tightly monitored to properly characterize the exposure. Organisms were exposed for 3 weeks and sampled weekly for biomarker analysis. Mussel filter-feeding activity resulted in significant removal of nCeO2 from the water column. At the same time, bioaccumulation was low, reaching its maximum in the first week. Mussels bioaccumulated ci-CeO2 three times more than ba-CeO2, probably due to coating-related differences in their behavior in the water column and in organisms. Meanwhile, biomarker results were integrated and synthesized using linear discriminant analysis, highlighting that pi-glutathione-S-transferase (piGST) mRNA, catalase (CAT) activity and lysosomal system were the most impacted of the seven biomarkers singled out by the discriminant analysis. These biomarker responses indicated that mussels exposed to both forms of nCeO2 were stressed and differentiate from the controls. Moreover, they responded differently to ba-CeO2 and ci-CeO2 exposure. However, biomarkers used in the experimental conditions of this study did not indicate severe nCeO2 toxicity on mussels, as cellular damage biomarkers and mussel filtering activity were left unimpaired. However, further studies are needed to investigate if the slight perturbations observed could lead to populational impacts in the long term.

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Section: Biomarker Measurements -Detailed Protocols In Smmentioning

confidence: 99%

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

et al. 2016

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“…Ciliates were observed between the interfilamentar spaces of the current work without obvious effects. The presence of microorganisms in the gills of the clams collected from the polluted area was reported in several studies (Neff et al 1987;Minguez et al, 2012;Carella et al, 2016). They discussed that pollution could compromise the animals` defence system and make it susceptible to parasites and invading organisms.…”

Section: Discussionmentioning

confidence: 96%

Histological Responses of The Freshwater Clam, Caelatura nilotica (Cailliaud, 1827) to Different Environmental Degrees Of Mixed Pollution

Sheir

2020

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Mixed pollution of the freshwaters is a worldwide problem; and the scientific evidence showed its impacts on the bivalves` biological processes. In the present study, the freshwater clam, Caelatura nilotica was collected from two water habitats with different degrees of pollution. Examination of gills and digestive gland using routine histology, scanning (SEM), and transmission (TEM) electron microscopy was done. In SEM investigations of the gills, deformation and erosion of cilia, clogging of gills by mucous and ghost gills were observed in the clams collected from a more polluted site. Histological structure of gills proved the appearance of ciliates between the gill filaments. Also, inflammatory reactions appeared and the epithelia lost their integrity and desquamated. The digestive gland showed haemocytes infiltrations, necrosis in some digestive tubules, and granulocytomas was observed. A full description of the digestive gland was done using TEM. In addition, more abnormalities were recorded in different types of the haemocytes, digestive and basophilic cells, flagella, microvilli, lysosomes, residual bodies, mitochondria, nucleus and chromatin with blebbing in most of them. In conclusion, mixed pollution compromised the functions of the gills and digestive gland of the clam, C. nilotica, under the effect of synergistic and/ or antagonistic actions of inorganic and organic contaminants with reference to the more severe effects of the non-point source than the point-source pollution in water pollution impacts.

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“…This process has been well studied for vertebrate hosts (reviewed in [17]) and only a few publications focused on molluscs, essentially gastropods and marine bivalves ([35], [36] and reviewed in [1]). On D. polymorpha , our team has worked on the physiological modifications linked with parasitism in a context of environmental disturbances [29], [30], [31], [32]. Nonetheless, to better understand the system zebra mussel - its parasites, it seemed interesting to have a more mechanistic approach of the host-parasite relationship, for example, by focusing on apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…in the digestive gland, and extracellular cysts of trematodes Phyllodistomum folium in gills and Bucephalus polymorphus in gonads. Our previous studies have shown that these parasites induced some physiological disturbances more or less important for their host [29], [30], [31], [32]. However, to better understand these host-parasite systems, it is important to go into the finer mechanisms underlying the establishment of the parasite.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Apoptosis in Host-Parasite Interactions in the Zebra Mussel

et al. 2013

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The question of whether cell death by apoptosis plays a biological function during infection is key to understanding host-parasite interactions. We investigated the involvement of apoptosis in several host-parasite systems, using zebra mussels Dreissena polymorpha as test organisms and their micro- and macroparasites. As a stress response associated with parasitism, heat shock proteins (Hsp) can be induced. In this protein family, Hsp70 are known to be apoptosis inhibitors. Mussels were diagnosed for their respective infections by standard histological methods; apoptosis was detected using the TUNEL methods on paraffin sections and Hsp70 by immunohistochemistry on cryosections. Circulating hemocytes were the main cells observed in apoptosis whereas infected tissues displayed no or few apoptotic cells. Parasitism by intracellular bacteria Rickettsiales-like and the trematode Bucephalus polymorphus were associated with the inhibition of apoptosis whereas ciliates Ophryoglena spp. or the trematode Phyllodistomum folium did not involve significant differences in apoptosis. Even if some parasites were able to modulate apoptosis in zebra mussels, we did not see evidence of any involvement of Hsp70 on this mechanism.

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Cross-effects of nickel contamination and parasitism on zebra mussel physiology

Cited by 14 publications

References 50 publications

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

Histological Responses of The Freshwater Clam, Caelatura nilotica (Cailliaud, 1827) to Different Environmental Degrees Of Mixed Pollution

Involvement of Apoptosis in Host-Parasite Interactions in the Zebra Mussel

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