Cross flow coupled with inertial focusing for separation of human sperm cells from semen and simulated TESE samples

Abstract

A triplet spiral channel coupled with cross-flow filtration has been designed and fabricated in an effort to separate sperm cells from either semen or simulated testicular sperm extraction (TESE) samples.

Label-free microfluidic cell sorting and detection for rapid blood analysis

“…[87][88][89] As these forces are particle size dependent, different cells are focused on distinct equilibrium positions to achieve continuous and high throughput (∼mL min −1 ) cell separation for different bio-fluids including blood, urine, 90 and semen samples. 91 When designing inertial cell sorters, it is important to consider both target and non-target cell sizes, starting sample concentration and volume, fluid properties, and channel dimensions for optimum focusing. 92 Abdulla et al reported a zigzag microchannel for label-free separation of CTCs from whole blood.…”

Inertial microfluidics: current status, challenges, and future opportunities

“…[83][84][85][86] Another strategy involves the coupling of inertial microfluidics with another passive or active manipulation technique; for example, spiral inertial microfluidics has been integrated with a membrane filter, 87 deterministic lateral displacement (DLD), 88,89 and cross-flow filtration. [90][91][92] In the case of the two-stage inertial-DLD (i-DLD) sorter, a high sample purity of 93.59% was achieved for the separation of MCF-7 cells from blood cells. 88 Although DLD has a high separation accuracy, the integrated device cannot eliminate the essence of size-dependent separation and is still unable to separate cells with similar or overlapping sizes.…”