Cross-immunity in Nile tilapia vaccinated with Streptococcus agalactiae and Streptococcus iniae vaccines

Wang, Qishuo; Fu, Tianzeng; Li, Xin-Cang; Luo, Qian; Huang, Jinjing; Sun, Yongcan; Wang, Xuepeng

doi:10.1016/j.fsi.2019.12.021

Cited by 40 publications

(21 citation statements)

References 44 publications

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“…The fundamental relationship between them is a competition between host immunity and bacterial pathogenicity (Pacheco et al., 2012; Pan et al., 2013; Wang, Ding et al, 2012; Wang, Yan et al, 2012; Wang, et al., 2020). For example, host immunity after vaccination was greater than the invading ability of bacteria; fish could not be dead appearing (Wang, Fu et al., 2020; Wang et al., 2018; Wang, Wang et al., 2020; Yan et al., 2018). As we know, E. piscicida is an important zoonotic pathogen.…”

Section: Discussionmentioning

confidence: 99%

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

Chen

et al. 2020

Journal of Fish Diseases

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Edwardsiella piscicida (E. piscicida) is an important fish pathogen. However, the mechanism of Glu6P transport regulatory protein UhpA how to affect the virulence gene expression in E. piscicida is still unclear. The results in this study showed that the metabolism‐related gene expression of cysteine synthase (orf 1134) and sulphate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76‐fold and 0.68‐fold lower than the ones in the wild strains (p < .05). The gene expression of ethA and ethB in the ΔuhpA strain was 0.80‐fold and 0.72‐fold lower than the ones in the wild strains (p < .05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51‐fold and 1.21‐fold higher than the ones in the wild strains (p < .05). The gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27‐fold, 1.13‐fold, 1.28‐fold and 1.23‐fold higher than the ones in the wild strains (p < .05). This suggested that the uhpA gene could regulate the key virulence gene expression, and the uhpA gene was associated with the pathogenicity of E. piscicida in fish.

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Section: Discussionmentioning

confidence: 99%

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

Chen

et al. 2020

Journal of Fish Diseases

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“…The results of this study show that a part of immune‐related genes expression in fish strongly induced by SIG. These pro‐inflammatory cytokines and immune‐related genes are considered an essential component of the host defence mechanism to defend the pathogenic bacteria (Wang, Fu, et al., 2020). In addition to being viable vaccine candidates, bacterial ghosts can be used as a carrier of drugs, nucleic acids, viral and/or bacterial antigens comprising multivalent vaccines designed to protect against diseases (Wang & Lu, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The local strain of S. iniae (GenBank No. ) used in this study was isolated in 2012 from the moribund tilapia on a farm in Taian, Shandong Province, China (Wang, Fu, et al., 2020). This isolate was cultured on brain heart infusion of bovine (BHI, HuanKai) at 28°C for 48 h. Colonies were cultured onto BHI agar at 28°C for 24 h. Staphylococcus aureus was cultured in tryptic soy broth (TSB; Difco) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Non‐specific immune parameters including acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) and lysozyme (LZM) activity in sera were determined using the detection kits according to the manufacturer's protocols (Wang, Fu, et al., 2020). The phagocytic activity of leucocytes was measured using the same method as our earlier research (Yan et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

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Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Wang

Zhang

et al. 2020

Aquaculture Research

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Streptococcus iniae (S. iniae) is a Gram‐positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG‐immunized) than group B (formalin‐inactivated S. iniae, FSI‐immunized), which was significantly higher than the control group (PBS‐treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL‐1β, IFN‐γ and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL‐1β and IFN‐γ in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI‐immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS‐treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge.

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“…Host-bacterial interactions are very complex, the fundamental relationship between them are competition between host immunity and bacterial pathogenicity (6,(16)(17)(18). For example, host immunity after vaccination was greater than the invading ability of bacteria; fish could not be dead appearing (4,19,20).…”

Section: Discussionmentioning

confidence: 99%

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Chen¹,

Sun²,

Ye³

et al. 2020

Preprint

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Edwardsiella piscicida (E. piscicida) is an important zoonotic pathogen, which infects animals by colonizing the intestine. Glucose 6-phosphate (Glu6P) was an important carbohydrate in intestine and could be used as a regulate signal. Here we identify a virulence-regulating pathway named Glu6P transport regulatory protein UhpA, which affects the virulent genes of hemolysins, flagellar, T3SS, T6SS and metabolism related genes how to promote E. piscicida infect the host. The results showed that the metabolism related gene expression of cysteine synthase (orf 1134) and sulfate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76-fold and 0.68-fold lower than the ones in the wild strains (P < 0.05), the gene expression of ethA and ethB in the ΔuhpA strain was 0.80-fold and 0.72-fold lower than the ones in the wild strains (P < 0.05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51-fold and 1.21-fold higher than the ones in the wild strains (P < 0.05), the gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27-fold, 1.13-fold 1.28-fold and 1.23-fold higher than the ones in the wild strains (P < 0.05). Besides, the survival rate of fish challenged with E. piscicida EIB202 and ΔuhpA was 50% and 30% respectively. These suggested that although the uhpA gene deletion decreased the metabolic level and the hemolysins related gene expression in E. piscicida, the uhpA gene could down regulate the key virulent gene expression to decrease the pathogenicity of E. piscicida in fish.

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Cross-immunity in Nile tilapia vaccinated with Streptococcus agalactiae and Streptococcus iniae vaccines

Cited by 40 publications

References 44 publications

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

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