Cross-linked stem bromelain: A more stabilized active preparation

Abstract

Cross-linking of the protease stem bromelain (bromelain) with 0.25 and 1.25% glutaraldehyde (GTA) results in the formation of a large molecular mass, multimeric and soluble aggregate having comparable activity to the unmodified bromelain. Both 0.25 and 1.25% GTA cross-linked (CL) bromelain preparations were more stable against urea, guanidine hydrochloride (GdnHCl) and temperature-induced inactivation, and exhibited slightly better storage stability compared to the unmodified protease. Such a high molecular weight, soluble, active and stable preparation may be useful in industry, i.e. in the textile industry for improving the properties of a fabric without loss of fabric strength and shape.

Stability, purification, and applications of bromelain: A review

“…Anwar et al showed that bromelain gradually loses its activity after 3 h of incubation at 60°C, pH 7.0, with 18% of the original activity remaining after this period. When the temperature was increased from 50 to 60°C, bromelain activity was found to be 1.6 times lower.…”

“…Therefore, it may be concluded that the molten globule state intermediate is essential for the denaturation of bromelain. 21 Anwar et al 22 showed that bromelain gradually loses its activity after 3 h of incubation at 608C, pH 7.0, with 18% of the original activity remaining after this period. When the temperature was increased from 50 to 608C, bromelain activity was found to be 1.6 times lower.…”

Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization

“…Currently, a number of different enzyme immobilization techniques are being used in industry such as enzyme adsorption, entrapment, and cross-linking (Sheldon, 2007). A particularly attractive method is attachment/adsorption of the enzyme to the surface of a carrier which commonly results in enhanced enzyme stability Anwar et al, 2007;López-Gallego et al, 2005;Mozhaev et al, 1990;Qiu et al, 2005;Rocchietti et al, 2004). Stability enhancements have been shown after attachment to many different carriers, examples are magnetic beads (Qiu et al, 2005), mesoporous sol-gel glass (Wang et al, 2001), silica nanospheres (Wang and Caruso, 2004), Sepabeads (Rocchietti et al, 2004), agarose (Rocchietti et al, 2004), and protein-coated microcrystals (Kreiner and Parker, 2005).…”

The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain

“…According to Anwar;Younus (2007), bromelain gradually lost its activity after 3h at 60 ºC, pH 7.0, and the remaining activity after incubation was 18%. That means, with the increase in temperature from 50 ºC to 60 ºC, bromelain lose 1.6-fold activity; since in our study, the remaining activity was around 29% at 50 ºC, pH 7.0, after 3 h incubation.…”