Cross‐linking of ADP‐ribosylated human translocation factor to ribosomes

Üçer, U.; Bermek, Engin

doi:10.1016/0014-5793(74)80104-3

Cited by 4 publications

References 26 publications

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Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

Nurten¹,

Aktar²,

Bermek³

1983

FEBS Letters

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Treatment of rat liver EF‐2 with N‐ethylmaleimide (MalNEt) did not affect the direct interactions of the factor with guanine nulceotides or with ribosomes, but inhibited the binding of guanosine 5′‐(β,γ‐methylene)triphosphate (GuoPP(CH2)P) to the EF‐2‐ribosome complex. The amino group reactive reagent 2,4,6‐trinitrobenzenesulfonate (TNBS), however, inhibited specifically the direct interactions of EF‐2 with guanine nucleotides, but not the binding of GuoPP(CH2)P to the EF‐2‐ribosome complex. The different sensitivities of EF‐2 to MalNEt and to TNBS suggested that the binding sites involved in the binary vs. ternary complex might correspond to different conformational states or might even be distinct physical entities.

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Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

Nurten¹,

Aktar²,

Bermek³

1983

FEBS Letters

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Mechanisms in Polypeptide Chain Elongation on Ribosomes

Bermek

1978

Progress in Nucleic Acid Research and Molecular Biology

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The Binding of Tritiated Elongation‐Factors 1 and 2 to Ribosomes from Krebs II Mouse Ascites‐Tumor Cells

Nolan

Grasmuk

Drews

1976

European Journal of Biochemistry

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The effect of a number of different antibiotics and toxins on the capacity of Krebs II mouse ascites ribosomes to bind 3H‐labelled elongation factors (EF‐1 and EF‐2) has been examined. It was found that abrin and ricin inhibit the binding of EF‐2, while diphtheria toxin, sparsomycin, streptovitacin A, and cycloheximide had essentially no effect on its binding. Of the other compounds examined, sparsomycin was unique in its capacity, under some circumstances, to significantly affect the binding of aminoacyl‐tRNA and EF‐1 to ribosomes. Fusidic acid appears to nonspecifically enhance the binding of both EF‐1 and EF‐2.

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Cross‐linking of ADP‐ribosylated human translocation factor to ribosomes

Cited by 4 publications

References 26 publications

Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

Mechanisms in Polypeptide Chain Elongation on Ribosomes

The Binding of Tritiated Elongation‐Factors 1 and 2 to Ribosomes from Krebs II Mouse Ascites‐Tumor Cells

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