Cross-linking the murine heat-stable antigen induces apoptosis in B cell precursors and suppresses the anti-CD40-induced proliferation of mature resting B lymphocytes.

Chappel, M. S.; Hough, Margaret R.; Mittel, A; Takei, Fumio; Kay, R.; Humphries, R. Keith

doi:10.1084/jem.184.5.1639

Cited by 44 publications

(56 citation statements)

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“…Consistent with our observations, Chappel et al demonstrated that the cross-linking of HSA, a mouse homolog of CD24, induces apoptosis in murine B cell precursors in BM (17) and concluded that HSA acts as a negative regulator of B cell precursors in a physiological context. Ligation of HSA may assist in the elimination of undesirable B cell precursors, such as cells with aberrant or nonproductive H chain and L chain gene rearrangements, or cells that display self-specificity (17). Our research allows their hypothesis to be expanded to include a potential role for CD24 as a negative regulator of B cell precursors in humans.…”

Section: Discussionsupporting

confidence: 79%

“…First, CD24 is known to mediate signal transduction, including phosphorylation of intracellular proteins and intracellular calcium mobilization (13)(14)(15). In addition, cross-linking of CD24 induces apoptosis in mouse B cell precursors (16,17). These findings suggest that CD24 is a signal-transducing molecule that acts as a potent negative regulator of B cell development.…”

mentioning

confidence: 86%

“…1A), as is the case with murine pre-B cells (17). When pre-B HPB-NULL cells were exposed to anti-CD24 mAb in the presence of secondary rabbit polyclonal anti-mouse Ig Ab, a significant number of annexin-V-bound cells appeared in a time-dependent manner (Fig.…”

Section: Cross-linking Of Cd24 Induces Apoptosis In Pre-b and Pro-b Amentioning

confidence: 97%

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Pre-B Cell Antigen Receptor-Mediated Signal Inhibits CD24-Induced Apoptosis in Human Pre-B Cells

Taguchi

Kiyokawa

Mimori

et al. 2003

The Journal of Immunology

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We previously reported that the cross-linking of cluster of differentiation (CD)24 induces apoptosis in Burkitt’s lymphoma cells and that this phenomenon can be enhanced by a B cell Ag receptor (BCR)-mediated signal. In this study, we extend our previous observation and report that CD24 also mediated apoptosis in human precursor-B acute lymphoblastic leukemia cell lines in the pro-B and pre-B stages accompanying activation of multiple caspases. Interestingly, simultaneous cross-linking of pre-BCR clearly inhibited CD24-mediated apoptosis in pre-B cells. We also observed that mitogen-activated protein kinases (MAPKs) were involved in the regulation of this apoptotic process. Pre-BCR cross-linking induced prompt and strong activation of extracellular signal-regulated kinase 1, whereas CD24 cross-linking induced the sustained activation of p38 MAPK, following weak extracellular signal-regulated kinase 1 activation. SC68376, a specific inhibitor of p38 MAPK, inhibited apoptosis induction by CD24 cross-linking, whereas anisomycin, an activator of p38 MAPK, enhanced the apoptosis. In addition, PD98059, a specific inhibitor of MEK-1, enhanced apoptosis induction by CD24 cross-linking and reduced the antiapoptotic effects of pre-BCR cross-linking. Collectively, whether pre-B cells survive or die may be determined by the magnitude of MAPK activation, which is regulated by cell surface molecules. Our findings should be important to understanding the role of CD24-mediated cell signaling in early B cell development.

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 86%

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Pre-B Cell Antigen Receptor-Mediated Signal Inhibits CD24-Induced Apoptosis in Human Pre-B Cells

Taguchi

Kiyokawa

Mimori

et al. 2003

The Journal of Immunology

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“…CD24, first attributed as a differentiation marker for B cells, is involved in lymphocyte maturation (2,3), regulates the proliferation of neuronal precursors (4), and was shown to be involved in the regulation of homeostatic cell renewal (5). CD24 is known to be overexpressed in various human malignancies, both solid and hematological (6,7), and is usually tied with a more aggressive course of the disease (1,8,9).…”

mentioning

confidence: 99%

The CD24 Protein Inducible Expression System Is an Ideal Tool to Explore the Potential of CD24 as an Oncogene and a Target for Immunotherapy in Vitro and in Vivo

Shapira

Kazanov²,

Weisblatt

et al. 2011

Journal of Biological Chemistry

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Background: It is critical to tightly regulate gene expression to study its biological role. Results: A tetracycline-dependent CD24 expression system was successfully implemented. An efficient study of its potential as an oncogene and a target for immunotherapy was performed. Conclusion: This is a valuable tool to study CD24 pathogenesis and novel treatment options for CD24-expressing malignancies. Significance: The CD24 inducible expression system allows accurate analysis of its role and function.

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“…Tümör hücrelerinin kan yoluyla metastaz yapması için, tümör hücrelerinin kan damarı içinde trombositlerle kümeleşmeleri korunması için çok önemli-dir. Ayrıca, CD24ün endotelle ve hücre dışı matriksle ilşkileri de tümörün metastaz yapma kabiliyetini arttırmaktadır (17).…”

Section: Discussionunclassified

The Correlation of CD24 Expression with Tumor Stage and Grade in Urothelial Carcinomas of the Urinary Bladder

Canberk¹,

Hak²,

Öznur³

et al. 2013

Istanbul Med J

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Amaç: B lenfosit belirteci olarak bilinen CD24, intrasellüler sinyal iletiminde, hücre-hücre ve hücre-matriks arasındaki haberleşmenin düzenlenme-sinde de önemli rol almaktadır. Son 10 yılda CD24 salınımı ile birçok tü-mörün ilerlemesi ve prognozu arasında ilişki bulunmuştur. Bu çalışmadaki amacımız, CD24 salınımının mesane ürotelyal karsinomlarında, en önem-li morfolojik prognostik parametreler olan tümör evresi ve diferansiyasyon derecesi ile karşılaştırmak ve etkinliğini tartışmaktır. Bulgular: CD24 salınımı ile invaziv (pT1-2) ve invaziv olmayan (pTa) olgular arasında istatistiksel olarak anlamlı bir farklılık bulundu (p<0,01). Normal ürotelyal epitelde ve tümöre komşu normal epitelde CD24 immünreaksiyo-nu, genelde apikalde zayıf sitoplazmik ve/veya membranöz boyanma şek-linde izlendi (skor 0 ve 1). Düşük dereceli ürotelyal tümörlerde ve normal ürotelyum epitelinde görülen apikal boyanma, invaziv tümörlerde kaybolmuştu ve nonpolarize kuvvetli sitoplazmik ve mebranöz boyanma izlendi. pTa (invaziv olmayan) ürotelyal karsinomlarda immün reaksiyon genelde zayıf ve daha çok apikal boyanma şeklinde izlendi, pTa yüksek dereceli olgularda da benzer boyanma izlenmekle beraber, pozitif olgularda immün reaktivite daha yaygın ve apikal boyanma polaritesi kaybolmasıyla birlikteydi. Düşük ve yüksek dereceli olgular arasında, CD24 immun reaksiyonu ile istatiksel olarak anlamlı farklılık bulunmuştur (p<0,05). YöntemlerSonuç: Çalışmamızda ürotelyal karsinomlarda özellikle apikal lokalizasyonlu sitoplazmik boyanmanın kaybı ile stromal invazyon arasında ilişki tespit ettik ve sonuç olarak; CD24 ün sitoplazmik aşırı salınımını tümör invazyonu için önemli bir belirteç olarak kullanılabileceğini düşünüyoruz. Ayrıca gelecekte immunoterapinin (anti-CD24 antikoru), mesane kanserlerinde ve CD24 salınımı yapan diğer kanserlerde kullanılabilabilir olacağını ve bu konuda ileri araştırmalara ihtiyaç duyulduğunu düşünmekteyiz.Anahtar Kelimeler: Ürotelyal karsinom, CD24, derece, evre, mesane Objective: Initially, CD24 was idendified as a B cell marker, in addition to a role of CD24 for the intracellular signaling, and it is also participates in the regulation of cell to cell and cell to matrix interactions. CD24 has been implicated in the progression of several types of cancer and has been identified as a prognostic factor in the last 10 years. We focused our attention on possible associations between CD24 expression and stage-grade of tumors in bladder urothelial carcinoma as important morphologic-prognostic paramaters.Methods: A total of 79 patients with bladder urothelial carcinomas were included in this study and these 79 patients underwent transurothelial resection biopsy at the Istanbul Education and research Hospital between 2005-2008. Hematoxylin-eosin stained sections from each case were reevaluated histopathologically according to the WHO-2004 grading system. The TNM system was used for pathologic staging. Selected slides were also studied by immunohistochemistry and a semiquantitative scoring for CD24 expression based on the per...

show abstract

Cross-linking the murine heat-stable antigen induces apoptosis in B cell precursors and suppresses the anti-CD40-induced proliferation of mature resting B lymphocytes.

Cited by 44 publications

References 45 publications

Pre-B Cell Antigen Receptor-Mediated Signal Inhibits CD24-Induced Apoptosis in Human Pre-B Cells

Pre-B Cell Antigen Receptor-Mediated Signal Inhibits CD24-Induced Apoptosis in Human Pre-B Cells

The CD24 Protein Inducible Expression System Is an Ideal Tool to Explore the Potential of CD24 as an Oncogene and a Target for Immunotherapy in Vitro and in Vivo

The Correlation of CD24 Expression with Tumor Stage and Grade in Urothelial Carcinomas of the Urinary Bladder

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