Cross-links between stereocilia in the guinea pig organ of Corti, and their possible relation to sensory transduction

Pickles, James O.; Comis, S. D.; Osborne, Michael P.

doi:10.1016/0378-5955(84)90041-8

Cited by 651 publications

(435 citation statements)

References 20 publications

Supporting

Mentioning

416

Contrasting

Unclassified

Order By: Relevance

“…The best-known application of the cellpoking assay is the version from auditory physiology, in which hair cell stereocilia are displaced to evoke an electrical signal (31). These experiments, along with specific molecular anatomical features of the stereocilia, underlie the famous tip-link tethergated transduction channel model (43,44), which, interestingly, has a more recent version in which the tether has been proposed to "tent" (i.e., exert tension on) the membrane surrounding the transduction channel (45). In the case of TRAAK and TREK channels it is clear that mechanosensitivity persists in the absence of potential tethers (Figs.…”

Section: Discussionmentioning

confidence: 99%

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels

Brohawn

MacKinnon

2014

Proc. Natl. Acad. Sci. U.S.A.

358

327

View full text Add to dashboard Cite

Mechanosensitive ion channels underlie neuronal responses to physical forces in the sensation of touch, hearing, and other mechanical stimuli. The fundamental basis of force transduction in eukaryotic mechanosensitive ion channels is unknown. Are mechanical forces transmitted directly from membrane to channel as in prokaryotic mechanosensors or are they mediated through macromolecular tethers attached to the channel? Here we show in cells that the K + channel TRAAK (K2P4.1) is responsive to mechanical forces similar to the ion channel Piezo1 and that mechanical activation of TRAAK can electrically counter Piezo1 activation. We then show that the biophysical origins of force transduction in TRAAK and TREK1 (K2P2.1) two-pore domain K + (K2P) channels come from the lipid membrane, not from attached tethers. These findings extend the "force-from-lipid" principle established for prokaryotic mechanosensitive channels MscL and MscS to these eukaryotic mechanosensitive K + channels.mechanosensation | potassium channel | gating | tension | reconstitution

show abstract

Section: Discussionmentioning

confidence: 99%

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels

Brohawn

MacKinnon

2014

Proc. Natl. Acad. Sci. U.S.A.

358

327

View full text Add to dashboard Cite

show abstract

“…Finally, tip links run obliquely from the ends of stereocilia to the sides of the longest adjacent stereocilia (Pickles et al 1984). These filaments are probably connected to mechanically sensitive channels and are therefore essential to mechanoelectrical transduction.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional Architecture of Hair-bundle Linkages Revealed by Electron-microscopic Tomography

Auer

Koster

Ziese

et al. 2008

JARO

View full text Add to dashboard Cite

The senses of hearing and balance rest upon mechanoelectrical transduction by the hair bundles of hair cells in the inner ear. Located at the apical cellular surface, each hair bundle comprises several tens of stereocilia and a single kinocilium that are interconnected by extracellular proteinaceous links. Using electron-microscopic tomography of bullfrog saccular sensory epithelia, we examined the three-dimensional structures of basal links, kinociliary links, and tip links. We observed significant differences in the appearances and dimensions of these three structures and found two distinct populations of tip links suggestive of the involvement of different proteins, splice variants, or protein-protein interactions. We noted auxiliary links connecting the upper portions of tip links to the taller stereocilia. Tip links and auxiliary links show a tendency to adopt a globular conformation when disconnected from the membrane surface.

show abstract

“…The first type of link is the tip link which extends from the tip of shorter stereocilia and attaches to the shaft of the adjacent taller stereocilia of the next row (Osborne et al ,1988Pickles et al 1984Pickles et al ,1989Comis et al 1985;Furness and Hackney 1985;Hackney et al 1988;Osborne and Comis 1990). In the tip-link model for transduction, the tip links act as gating springs for mechanically sensitive ion channels (Hudspeth 1989;Pickles and Corey 1992).…”

mentioning

confidence: 99%

Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

Tsuprun

Santi

2002

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R YThe structure and symmetry of chinchilla outer hair cell (OHC) stereocilia side and attachment links were investigated by transmission electron microscopy using tannic acid and Cuprolinic blue histochemical procedures. The side links run laterally between and across the rows of the stereocilia and connect the stereocilia together within the bundle. Attachment links form a crown-like array around the tips of only the tallest OHC stereocilia and attach these stereocilia to the Type B fibrils of the tectorial membrane. Computer averaging of the side links from tannic acid-treated tissues showed a central dense region of the link between adjacent stereocilia and a smaller dense portion at the plasma membrane end of the link. Computer averaging of Cuprolinic blue-treated tissues showed low electron density of the central region of the link, and the plasma membrane ends of the link were electron dense. After tannic acid treatment, the attachment links showed a diffused radial distribution around the tips of the tallest OHC stereocilia. After Cuprolinic blue treatment, the attachment links appeared as electron-dense, membrane-bound granular structures arranged with radial symmetry. The central regions of the side links are reactive to tannic acid. These regions appear to contain neutral and basic residues of proteins and participate in side-by-side association of the side links in regular aggregates. Cuprolinic blue-reactive regions of the side and attachment links appear to contain acidic sulfated residues of glycoproteins or proteoglycans, which may be involved in the attachment of these links to the stereocilium membrane.

show abstract

Cross-links between stereocilia in the guinea pig organ of Corti, and their possible relation to sensory transduction

Cited by 651 publications

References 20 publications

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels

Three-dimensional Architecture of Hair-bundle Linkages Revealed by Electron-microscopic Tomography

Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

Contact Info

Product

Resources

About

Cross-links between stereocilia in the guinea pig organ of Corti, and their possible relation to sensory transduction

Cited by 651 publications

References 20 publications

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K + channels

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K + channels

Three-dimensional Architecture of Hair-bundle Linkages Revealed by Electron-microscopic Tomography

Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

Contact Info

Product

Resources

About

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels

Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K ⁺ channels