Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom

Beghini, Daniela Gois; Cruz‐Höfling, Maria Alice da; Randazzo-Moura, Priscila; Rodrigues‐Simioni, Léa; Novello, José Camillo; Hyslop, Stephen; Marangoni, Sérgio

doi:10.1016/j.toxicon.2005.06.018

Cited by 23 publications

(9 citation statements)

References 29 publications

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“…For example, the commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the typical neuromuscular blockade caused by B. jararacussu venom and BthTX-I. These results showed that antiserum produced against PLA 2 , the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA 2 toxins [123]. PLA 2 s can undergo chemical modifications at different amino acid residues according to the used reagent.…”

Section: Other Types Of Pla 2 Inhibitors As Bms-229724 (4-[4-[2-[2-[bmentioning

confidence: 88%

Snake Venom Phospholipase A2 Inhibitors: Medicinal Chemistry and Therapeutic Potential

Marcussi¹,

Sant’Ana²,

Oliveira³

et al. 2007

CTMC

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Phospholipases A2 (PLA2s) are commonly found in snake venoms from Viperidae, Hydrophidae and Elaphidae families and have been extensively studied due to their pharmacological and physiopathological effects in living organisms. This article reports a review on natural and artificial inhibitors of enzymatic, toxic and pharmacological effects induced by snake venom PLA2s. These inhibitors act on PLA2s through different mechanisms, most of them still not completely understood, including binding to specific domains, denaturation, modification of specific amino acid residues and others. Several substances have been evaluated regarding their effects against snake venoms and isolated toxins, including plant extracts and compounds from marine animals, mammals and snakes serum plasma, in addition to poly or monoclonal antibodies and several synthetic molecules. Research involving these inhibitors may be useful to understand the mechanism of action of PLA2s and their role in envenomations caused by snake bite. Furthermore, the biotechnological potential of PLA2 inhibitors may provide therapeutic molecular models with antiophidian activity to supplement the conventional serum therapy against these multifunctional enzymes.

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Section: Other Types Of Pla 2 Inhibitors As Bms-229724 (4-[4-[2-[2-[bmentioning

confidence: 88%

Snake Venom Phospholipase A2 Inhibitors: Medicinal Chemistry and Therapeutic Potential

Marcussi¹,

Sant’Ana²,

Oliveira³

et al. 2007

CTMC

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show abstract

“…In this context, Stabeli et al [47] showed that antibodies that recognize a peptide (Ile1-Hse11) from Bm-LAAO present crossed immunoreactivity with components not related to the LAAOs group present in venoms from Bothrops, Crotalus, Micrurus e Lachesis snake venoms. Also, Beghini et al [48] showed that the serum produced against crotoxin and phospholipase A2 from Crotalus durissus cascavella was able to neutralize the neurotoxic activity produced by B. jararacussu venom and BthTX-I.…”

Section: Midca1mentioning

confidence: 99%

Purification of Phospholipases A2 from American Snake Venoms

Stábeli¹,

Simões-Silva²,

Kayano³

et al. 2012

Chromatography - The Most Versatile Method of Chemical Analysis

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“…In the envenomation context, the question of the efficiency of specific bothropic antivenom against B. jararacussu bites is often discussed, since the myotoxic effects observed in mice are not completely neutralized. For this reason, some researchers have suggested the use of a combinated bothropic-crotalic antivenom as a more appropriate treatment [1, 6–10]. …”

Section: Introductionmentioning

confidence: 99%

Can anti-bothropstoxin-I antibodies discriminate between Bothrops jararaca and Bothrops jararacussu venoms?

Araujo

Corrêa-Netto

Brazil-Más

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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BackgroundSnakes of the genus Bothrops, popularly known as pit vipers, are responsible for most cases of snakebite in Brazil. Within this genus, Bothrops jararacussu and B. jararaca deserve special attention due to the severity of their bites and for inhabiting densely populated areas. Regarding the treatment of snakebites by Bothrops jararacussu, questions have been raised about the effectiveness of the specific bothropic antivenom in neutralizing myotoxic effects; however, there are no accurate data for humans. Thus, the development of a differential diagnostic kit for this species would be of great interest because it provides, for healthcare professionals, a tool that would allow us to determine whether the accident was caused by B. jararacussu or other species of the genus. It would also make it possible to evaluate the specificity of the treatment and to provide data for epidemiological studies.MethodsFirst, we produced a species-specific polyclonal antibody – a potential biomarker of Bothrops jararacussu venom – against bothropstoxin-I (BthTx-I), which is also found in smaller quantities in the venoms of B. jararaca from southern Brazil.ResultsPolyclonal antibodies against bothropstoxin-I could be separated into several species-specific immunoglobulins. Then, aiming to develop a system of safe and standardized immunoassay, we produced monoclonal antibodies. Seven hybridomas were obtained. Five of them were specific to the venom of B. jararacussu and two recognized the venom of B. jararaca from the southeastern population. The use of monoclonal antibodies also made it possible to differentiate B. jararacussu from B. jararaca venom obtained from the southern population. Analyzing the reactivity of monoclonal antibodies against other bothropic venoms, we found mAb Bt-3 to be more specific than others for B. jararacussu venom.ConclusionsThese results show the potential of BthTx-I for producing monoclonal antibodies that differentiate between B. jararacussu and other Bothrops species venoms.

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Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom

Cited by 23 publications

References 29 publications

Snake Venom Phospholipase A2 Inhibitors: Medicinal Chemistry and Therapeutic Potential

Snake Venom Phospholipase A2 Inhibitors: Medicinal Chemistry and Therapeutic Potential

Purification of Phospholipases A2 from American Snake Venoms

Can anti-bothropstoxin-I antibodies discriminate between Bothrops jararaca and Bothrops jararacussu venoms?

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