Cross-Platform Comparison of <i>Caenorhabditis elegans</i> Tissue Extraction Strategies for Comprehensive Metabolome Coverage

Geier, Florian; Want, Elizabeth J.; Leroi, Armand M.; Bundy, Jacob G.

doi:10.1021/ac2001109

Cited by 109 publications

(95 citation statements)

References 52 publications

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“…For GC-TOF/MS analysis, the CA1 region of hippocampus was isolated, weighed and extracted with 10 volumes of methanol-chloroform (3: 1, v/v) in Eppendorf tubes [25]. The mixture was homogenized at 65 Hz for 3 min and centrifuged at 4°C at 12,000 rpm for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Chronic Stress Contributes to Cognitive Dysfunction and Hippocampal Metabolic Abnormalities in APP/PS1 Mice

Han

Wang

Geng³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Stress response is determined by the brain, and the brain is a sensitive target for stress. Our previous experiments have confirmed that once the stress response is beyond the tolerable limit of the brain, particularly that of the hippocampus, it will have deleterious effects on hippocampal structure and function; however, the metabolic mechanisms for this are not well understood. Methods: Here, we used morris water maze, elisa and gas chromatography-time of flight/mass spectrometry to observe the changes in cognition, neuropathology and metabolomics in the hippocampus of APP/PS1 mice and wild-type (C57) mice caused by chronic unpredictable mild stress (CUMS), we also further explored the correlation between cognition and metabolomics. Results: We found that 4 weeks of CUMS aggravated cognitive impairment and increased amyloid-β deposition in APP/PS1 mice, but did not affect C57 mice. Under non-stress conditions, compared with C57 mice, there were 8 different metabolites in APP/PS1 mice. However, following CUMS, 3 different metabolites were changed compared with untreated C57 mice. Compared to APP/PS1 mice, there were 7 different metabolites in APP/PS1+CUMS mice. Among these alterations, 3-hydroxybutyric acid, valine, serine, beta-alanine and o-phosphorylethanolamine, which are involved in sphingolipid metabolism, synthesis and degradation of ketone bodies, and amino acid metabolism. Conclusion: The results indicate that APP/PS1 mice are more vulnerable to stress than C57 mice, and the metabolic mechanisms of stress-related cognitive impairment in APP/PS1 mice are related to multiple pathways and networks, including sphingolipid metabolism, synthesis and degradation of ketone bodies, and amino acid metabolism.

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Section: Methodsmentioning

confidence: 99%

Chronic Stress Contributes to Cognitive Dysfunction and Hippocampal Metabolic Abnormalities in APP/PS1 Mice

Han

Wang

Geng³

et al. 2017

Cell Physiol Biochem

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“…Because the diet of C. elegans can be easily manipulated by feeding with different E. coli strains, analysis of lipid species and other metabolites will shed light on how dietary factors affect fat storage in lipid droplets ( 34,56,87,88 ). Cell biological studies can also be coupled with powerful analytical methods that allow simultaneous monitoring of fatty acid absorption, elongation, and de novo synthesis in C. elegans , which have yet to be employed in other metazoans ( 89 ).…”

Section: Perspectivesmentioning

confidence: 99%

Lipid droplets as fat storage organelles in Caenorhabditis elegans

Mak

2012

Journal of Lipid Research

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This article is available online at http://www.jlr.org droplet size until a dominant unilocular lipid droplet remains ( 9, 10 ). The mechanisms that govern lipid droplet size and number in white adipose cells and other cell types are not fully understood.To study regulators of fat storage and lipid droplet size in metazoans, most studies have focused on the use of tissue culture cells and overexpressed lipid droplet-associated proteins or vital dyes as markers. These studies yielded important insights into how conserved proteins and signaling pathways are coupled to control cellular fat storage. Nevertheless, extension of observations made in tissue culture cells into whole-animal models is often time consuming and can lead to surprising results. In the last few years, C. elegans has emerged as an attractive model for studying fat storage in vivo ( 11,12 ). Although C. elegans lacks dedicated adipose tissues, its intestine serves as the main site for fat storage. Yolk lipoproteins are synthesized in the intestine, exported into the body cavity (pseudocoelomic space), and taken up by developing oocytes (13)(14)(15). This is remarkably similar to yolk deposition in chicken, where yolk is synthesized in the liver and transported to the ovum via the bloodstream ( 16 ). Therefore, the C. elegans intestine may be viewed as a multifunctional organ that fulfi lls the roles of the liver and adipose tissues.Inspection of the sequenced genome of C. elegans and functional studies through analysis of genetic mutants revealed extensive conservation of genes involved in fatty acid synthesis, elongation, desaturation, and degradation ( 17-23 ). Furthermore, fat storage in C. elegans appears to be controlled by conserved insulin, TGF-␤ , serotonin, and mammalian target of rapamycin (mTOR) signaling pathways ( 24-29 ). A number of reverse genetic techniques will accelerate functional dissection of signaling and protein interaction networks ( 30-32 ). The development of novel Lipid droplets are ubiquitous fat storage organelles that are conserved in yeast, C. elegans , Drosophila , and mammals ( 1-3 ). They are also sites of regulated release of stored fat by lipases during cell growth and fasting ( 4 ). Therefore, lipid droplets are central to energy balance at cellular and organismal levels. Key structural and biochemical features of lipid droplets have been defi ned, and it is generally accepted that neutral lipids such as triglycerides (TAG) and cholesterol esters (CE) are stored in the interior of the organelle that is delimited by a phospholipid monolayer ( 5-7 ). Current models suggest that fatty acid infl ux can be accommodated by neutral lipid synthesis and a concomitant change in lipid droplet size or number in a tissue-specifi c manner ( 1,8 ). For example, differentiation of mammalian white adipose cells is characterized by a decrease in lipid droplet number and an increase in lipid

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“…Recent advances in the analytical platforms of mass spectrometry (MS) and nuclear magnetic resonance (NMR) have driven the discipline of omics (Beckonert et al, 2010;Geier et al, 2011;Halama et al, 2011;Hrydziuszko et al, 2011;Jä ger et al, 2011;Kim et al, 2011;Kronthaler et al, 2012;Wu et al, 2009;Villas-Bô as and Bruheim, 2007;Zhang et al, 2012a). Technological advances have opened a new chapter in TCM by using chinmedomics as an approach to study the metabolomics of chinmediformulae.…”

Section: Emerging Trends and Potential Value Of Chinmedomicsmentioning

confidence: 99%

Future Perspectives of Chinese Medical Formulae: Chinmedomics as an Effector

Wang

Zhang

Sun

2012

OMICS: A Journal of Integrative Biology

159

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Traditional Chinese medicine (TCM) has been used for thousands of years to treat or prevent disease. The health care paradigm has shifted from a focus on disease to TCM therapy with a holistic approach. However, the actual value of TCM has not been fully recognized worldwide due to a lack of scientific approaches to its study. Today omics has become practically available, and resembles TCM in many aspects, and can serve as a key driving force for the translation of the traditional Chinese medical formulae (chinmediformulae) into practice, and will develop and advance the concept of the metabolomics of chinmediformulae (chinmedomics). Chinmedomics seeks to elucidate the therapeutic and synergistic properties and metabolism of chinmediformulae and the involved metabolic pathways using modern analytical techniques. It is an integral part of top-down systems biology, which aims to improve understanding of chinmediformulae. This approach of combining chinmedomics with chinmediformulae with modern health care systems may lead to a revolution in TCM therapy. Although the scientific study of chinmedomics is at an early stage and requires further scrutiny and validation, the approach has major implications to improve the efficacy of chinmediformulae. This article introduces and reviews the concept of chinmedomics, and highlights recent examples of the approach, which are presented for description and discussion.

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Cross-Platform Comparison of Caenorhabditis elegans Tissue Extraction Strategies for Comprehensive Metabolome Coverage

Cited by 109 publications

References 52 publications

Chronic Stress Contributes to Cognitive Dysfunction and Hippocampal Metabolic Abnormalities in APP/PS1 Mice

Chronic Stress Contributes to Cognitive Dysfunction and Hippocampal Metabolic Abnormalities in APP/PS1 Mice

Lipid droplets as fat storage organelles in Caenorhabditis elegans

Future Perspectives of Chinese Medical Formulae: Chinmedomics as an Effector

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