Cross‐Platform DNA Encoding for Single‐Cell Imaging of Gene Expression

Zrazhevskiy, Pavel; Akilesh, Shreeram; Tai, Wanyi; Queitsch, Konstantin; True, Lawrence D.; Fromm, Jonathan R.; Wu, David; Nelson, Peter S.; Stamatoyannopoulos, J; Gao, Xiaohu

doi:10.1002/anie.201603945

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…Annealed siRNA with 3′-TT overhangs was purchased from IDT (Coralville, IA). The sense strand sequence is 5′-CAUCAUCCCUGCCUCUACUTT-3′ 28 . HeLa cells were grown in a 10 cm TC-treated dish, trypsinized, and mixed in suspension with culture medium containing 25 nM GAPDH siRNA, together with 0.5 μl per well DharmaFECT-2 transfection reagent (Dharmacon).…”

Section: Methodsmentioning

confidence: 99%

Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine

Baird

Davis

et al. 2017

Nat Biomed Eng

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The ability to detect biomarkers with ultrahigh sensitivity radically transformed biology and disease diagnosis. However, owing to incompatibilities with infrastructure in current biological and medical laboratories, recent innovations in analytical technology have not received broad adoption. Here, we report a simple, universal ‘add-on’ technology (dubbed EASE) that can be directly plugged into the routine practices of current research and clinical laboratories and that converts the ordinary sensitivities of common bioassays to extraordinary ones. The assay relies on the bioconjugation capabilities and ultrafast and localized deposition of polydopamine at the target site, which permit a large number of reporter molecules to be captured and lead to detection-sensitivity enhancements exceeding 3 orders of magnitude. The application of EASE in the enzyme-linked-immunosorbent-assay-based detection of the HIV antigen in blood from patients leads to a sensitivity lower than 3 fg ml−1. We also show that EASE allows for the direct visualization, in tissues, of the Zika virus and of low-abundance biomarkers related to neurological diseases and cancer immunotherapy.

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Section: Methodsmentioning

confidence: 99%

Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine

Baird

Davis

et al. 2017

Nat Biomed Eng

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“…Zrazhevskiy et al designed orthogonal docking strands targeting each to the glyceraldehyde‐3‐phosphate dehydrogenase protein (GADPH) and heat shock protein 90 (HSP90), messenger RNA (mRNA), and small‐interfering RNA (siRNA). The corresponding imager strands (fully complementary to docking strands) were conjugated with different uniquely emitting QDs such that the molecular targets were easily and simultaneously identifiable by the respective QD emission wavelength using fluorescence microscopy and hyperspectral imaging ( Figure ) . QDs are also being developed as potential super resolution labels due to their blinking and ability to engage in switchable emission with photochromic FRET acceptor dyes…”

Section: Dna Nanostructures In Bioimagingmentioning

confidence: 99%

“…Shown here are false‐colored four hyperspectral microscopy scan images and a merged image of QD fluorescence from the four protein and mRNA subcellular components. Reproduced with permission . Copyright 2016, John Wiley and Sons.…”

Section: Dna Nanostructures In Bioimagingmentioning

confidence: 99%

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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DNA self‐assembly has proven to be a highly versatile tool for engineering complex and dynamic biocompatible nanostructures from the bottom up with a wide range of potential bioapplications currently being pursued. Primary among these is healthcare, with the goal of developing diagnostic, imaging, and drug delivery devices along with combinatorial theranostic devices. The path to understanding a role for DNA nanotechnology in biomedical sciences is being approached carefully and systematically, starting from analyzing the stability and immune‐stimulatory properties of DNA nanostructures in physiological conditions, to estimating their accessibility and application inside cellular and model animal systems. Much remains to be uncovered but the field continues to show promising results toward developing useful biomedical devices. This review discusses some aspects of DNA nanotechnology that makes it a favorable ingredient for creating nanoscale research and biomedical devices and looks at experiments undertaken to determine its stability in vivo. This is presented in conjugation with examples of state‐of‐the‐art developments in biomolecular sensing, imaging, and drug delivery. Finally, some of the major challenges that warrant the attention of the scientific community are highlighted, in order to advance the field into clinically relevant applications.

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“…The limitations of bulk cell analysis have motivated the development of single cell analysis. In contrast to the bulk cell analysis, single cell analysis reveals the significant physiological characteristics of an individual cell, such as metabolism [8], protein level [9] and gene expressions [10].…”

Section: Introductionmentioning

confidence: 99%

Recent Development of Microfluidic Technology for Cell Trapping in Single Cell Analysis: A Review

Deng

Guo

2020

Processes

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Microfluidic technology has emerged from the MEMS (Micro-Electro-Mechanical System)-technology as an important research field. During the last decade, various microfluidic technologies have been developed to open up a new era for biological studies. To understand the function of single cells, it is very important to monitor the dynamic behavior of a single cell in a living environment. Cell trapping in single cell analysis is urgently demanded There have been some review papers focusing on drug screen and cell analysis. However, cell trapping in single cell analysis has rarely been covered in the previous reviews. The present paper focuses on recent developments of cell trapping and highlights the mechanisms, governing equations and key parameters affecting the cell trapping efficiency by contact-based and contactless approach. The applications of the cell trapping method are discussed according to their basic research areas, such as biology and tissue engineering. Finally, the paper highlights the most promising cell trapping method for this research area.

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Cross‐Platform DNA Encoding for Single‐Cell Imaging of Gene Expression

Cited by 11 publications

References 27 publications

Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine

Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Recent Development of Microfluidic Technology for Cell Trapping in Single Cell Analysis: A Review

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