Fundus photography is now an established tool for showing early changes in the retinal nerve fibre layer (RNFL). RNFL defects may precede glaucomatous visual field defects by up to five years and any improvement in photography should aid RNFL defect analysis. We have found that the use of an unsharp mask has helped to visualise the RNFL and by using readily available darkroom materials the process of unsharp mask preparation has been simplified.
Key words:Fundus photography, high magnification, large format film, retinal nerve fibre layer, unsharp mask.Damage to the retinal nerve fibre layer (RNFL) may be the first visible sign of pathology in chronic glaucoma.',2 Critical examination of the RNFL3 relies on the use of high quality red-free photog r a p h~.~ Photography of the RNFL uses spectral reflection of red-free light from the superficial retina. ' Image quality can be improved by various combinations of filmy6 filter^^.^ proce~sing,~ by varying the and by the use of polarising filters.13 The aim of any change in photographic technique is to gain contrast without loss of information in the fine detail of the nerve fibre bundles. Astronomical photography has for many years used the photographic technique of unsharp masking to examine star systems in galaxies. l4 Similarly, photographic subtraction has been in use in radiology since 193S5 and has been suggested for use in fluorescein angi~graphy'~.'~ and monochromatic fundus and RNFL photography.18An RNFL negative contains a large range of densities which are often difficult to transfer to photographic paper or film unless darkroom techniques such as 'dodging' or 'burning-in' are used. These techniques are difficult to control, are time consuming and are not precisely repeatable without computerised enlarger control.Unsharp masking has the advantage of being simple and reproducible without the disadvantage of changing the original negative. This paper describes a simplified technique of unsharp masking for RNFL prints and transparencies. Unsharp masking is a photographic method of compressing the tonal range of a negative and improving fine detail.
MethodsIn order to reduce the variables in the preparation of an unsharp mask the contrast of films, unsharp masks and optimum unsharp mask density were determined by computerised image analy~is.'~ We have attempted to simplify the processing of the unsharp mask by using chemicals and materials available in most darkrooms so that enhanced RNFL images can be conveniently prepared.Our current method for RNFL photography consists of a Zeiss FF4 fundus camera, Kodak Technical pan 2415 film and a Zeiss RI red-free