Cross-pollination effects on morphological, molecular, and biochemical diversity of a selected cinnamon (<i>Cinnamomum zeylanicum</i>Blume) seedling population

Liyanage, Nishadi Maduwanthi Neluwa; Ranawake, A. L.; Bandaranayake, P. C. G.

doi:10.1080/15427528.2020.1795769

Cited by 13 publications

(13 citation statements)

References 26 publications

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“…Hydrated pollens deposited on the stigmatic papillae were observed. This is supported by a recent field study [64]. Of the Sri Wijaya offspring resulting from a single open-pollinated event, 20% of individuals shared identical ISSR fingerprints with the mother plant.…”

Section: Discussionsupporting

confidence: 63%

Macroscopic and microscopic study on floral biology and pollination of Cinnamomum verum Blume (Sri Lankan)

Hathurusinghe

Pushpakumara

Bandaranayake

2022

Preprint

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Cinnamomum verum Blume (syn Cinnamomum zeylanicum) commonly known as Ceylon cinnamon, has gained worldwide attention and well supported by experimental data. Maintaining the yield quality and quantity are essential, especially for high-end value-added products. Breeding superior varieties and producing good quality planting materials are critical for commercial cultivation efforts. However, limited literature is available on the floral development and biology of C. verum. We assessed the seasonal flowering, floral development and pollination of type A and type B varieties of C. verum. Both macroscopic and microscopic data were collected on floral biology, pollination, male and female floral organs before and after pollination. Cinnamomum verum is morpho-anatomically, structurally, and physiologically adapted for cross-pollination, possible between the two cultivars; type A (Sri Gemunu) and type B (Sri Wijaya) flowers; naturally evolved with Protogynous Dichogamy. It possesses a dry stigma, which changes its surface characteristics after pollination. During close-pollination, a higher percentage of pollen shrink and shrivel on stigma while it develops callose plugs to obstruct the growth of the pollen tube. However, during changes in environmental conditions, female and male stages in the same tree overlap for about 45-60 min suggesting possible close-pollination within the same plant. Moreover, 4-8% of the flowers formed fruits after natural close and hand pollination. pollen was found fully hydrated even after close-pollination. Although C. verum is adapted for cross-pollination, natural close-pollination is also possible. The data suggest the complex nature of the sexual reproduction of C. verum. Well-managed breeding attempts will develop superior C. verum varieties.

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Section: Discussionsupporting

confidence: 63%

Macroscopic and microscopic study on floral biology and pollination of Cinnamomum verum Blume (Sri Lankan)

Hathurusinghe

Pushpakumara

Bandaranayake

2022

Preprint

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“…Probably both accession 002 and 003 were originated from seeds collected from single mother plant. Our recent work showed that C. zeylanicum offspring resulted from a single cross pollination event are genetically and morphologically divese [53]. Nevertheless, HPLC and GC-MS analysis suggest all C. sinharajaense accessions and C. verum accessions consist of very similar chemical profiles except the differences in concentrations.…”

Section: Plos Onementioning

confidence: 92%

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

et al. 2021

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The genus Cinnamomum consists of about 250 species spread globally. Out of these, C. verum (C. zeylanicum), also known as true cinnamon or Ceylon cinnamon, has gained worldwide attention due to its culinary uses and medicinal values. Sri Lanka is the largest true cinnamon producer in the world and accounts for about 80–90% of global production. Other than the cultivated species, Sri Lankan natural vegetation is home to seven endemic wild species of the genus Cinnamomum. While these are underutilized, proper identification and characterization are essential steps in any sustainable conservation and utilization strategies. Currently, species identification is purely based on morphological traits, and intraspecific diversity has made it more challenging. In this study, all the eight Cinnamomum species found in Sri Lanka, C. capparu-coronde, C. citriodorum C. dubium, C. litseifolium, C. ovalifolium, C. rivulorum, C. sinharajaense, and C. verum were collected in triplicates and identified using typical morphological traits. DNA extracted with the same collection was assessed with universal barcoding regions, rbcL, matK, and trnH-psbA. While no intraspecific sequence differences were observed in C. citriodorum, C. rivulorum, and C. verum, the others had polymorphic sites in one, two, or all regions assessed. Interestingly, two individuals of C. sinharajaense had identical barcodes to the cultivated species C. verum, while the other one had one variable cite in matK region and three cites in trnH-psbA reigon. Further, one C. dubium and one C. capparu-coronde accession each had identical, rbcL, and trnH-psbA sequences while those had only a single nucleotide variation observed in matK region. Overall, the phylogeny of Cinnamomum species found in Sri Lanka could not be completely resolved with DNA barcoding regions studied.

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“…Therefore, one should consider that these chemotypes may result from the plant genotype, considering the season, the weather, and the collection site [ 35 ]. Eugenol is the most common chemotype identified in C. verum leaves [ 18 , 33 , 36 ]. In Sri Lanka, Malaysia, Cameroon, and India, the eugenol concentrations of the leaves of C. verum are around 80–90% [ 32 , 33 , 37 , 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

et al. 2022

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Cinnamomum verum (Lauraceae), also known as “true cinnamon” or “Ceylon cinnamon” has been widely used in traditional folk medicine and cuisine for a long time. The systematics of C. verum presents some difficulties due to genetic variation and morphological similarity between other Cinnamomum species. The present work aimed to find chemical and molecular markers of C. verum samples from the Amazon region of Brazil. The leaf EOs and the genetic material (DNA) were extracted from samples cultivated and commercial samples. The chemical composition of the essential oils from samples of C. verum cultivated (Cve1-Cve5) and commercial (Cve6-c-Cv9-c) was grouped by multivariate statistical analysis of Principal Component Analysis (PCA). The major compounds were rich in benzenoids and phenylpropanoids, such as eugenol (0.7–91.0%), benzyl benzoate (0.28–76.51%), (E)-cinnamyl acetate (0.36–32.1%), and (E)-cinnamaldehyde (1.0–19.73%). DNA barcodes were developed for phylogenetic analysis using the chloroplastic regions of the matK and rbcL genes, and psbA-trnH intergenic spacer. The psbA-trnH sequences provided greater diversity of nucleotides, and matK confirmed the identity of C. verum. The combination of DNA barcode and volatile profile was found to be an important tool for the discrimination of C. verum varieties and to examine the authenticity of industrial sources.

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Cross-pollination effects on morphological, molecular, and biochemical diversity of a selected cinnamon (Cinnamomum zeylanicumBlume) seedling population

Cited by 13 publications

References 26 publications

Macroscopic and microscopic study on floral biology and pollination of Cinnamomum verum Blume (Sri Lankan)

Macroscopic and microscopic study on floral biology and pollination of Cinnamomum verum Blume (Sri Lankan)

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

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