Cross-priming amplification for detection of bovine viral diarrhoea virus species 1 and 2

Abstract

This is the first report on the application of the CPA method for the detection of BVDV.

Development of cross-priming amplification for direct detection of the African Swine Fever Virus, in pig and wild boar blood and sera samples

“…The CPA was highly sensitive and may be conducted in a waterbath, without the application of thermocyclers (Kuta et al . ; Woźniakowski et al . ).…”

“…The presented CPA does not require DNA extraction, except 10-fold dilution in an APO buffer. The CPA was highly sensitive and may be conducted in a waterbath, without the application of thermocyclers (Kuta et al 2015;Wo zniakowski et al 2015). CPA was also capable of specifically detecting ASFV DNA in blood and sera, directly collected from swine and wild boars.…”

“…) or bovine viral diarrhoea virus (Kuta et al . ). In brief, CPA requires a set of 3–8 complementary primers, which recognizes specific loci, within identified DNA and uses polymerase with an isothermal effect, such as Bst , Bsm or Gsp SSD, exhibiting an additional, strand‐displacement effect.…”

“…Recently, CPA has been developed for the identification of different pathogens, such as Myco. tuberculosis (Fang et al 2009), avian reovirus , fowl adenovirus , penaeid shrimp white spot syndrome virus (Yang et al 2014) or bovine viral diarrhoea virus (Kuta et al 2015). In brief, CPA requires a set of 3-8 complementary primers, which recognizes specific loci, within identified DNA and uses polymerase with an isothermal effect, such as Bst, Bsm or GspSSD, exhibiting an additional, stranddisplacement effect.…”

TaqMan probe qRT-PCR detects bovine parvovirus and applies clinically

“…Currently, several methods have been utilized to detect BPV antigens [9,10]. However, each method has its own strengths and weaknesses in terms of sensitivity and turnaround time [11]. Real-time PCR offers the same advantages as conventional PCR assays, but it is much more rapid and sensitive due to real-time quantification of the data and visualization [12].…”