Cross-protection study of the nine serovars of<i>Haemophilus paragallinarum</i>in the Kume haemagglutinin scheme

Soriano, Edgardo V.; Garduño, Manuel Longinos; Téllez, G.; Rosas, Pomposo Fernández; Suárez-Güemes, Francisco; Blackall, P. J.

doi:10.1080/03079450400003502

Cited by 41 publications

(19 citation statements)

References 19 publications

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“…As all controls were fully susceptible to challenge and as no antibodies to infectious coryza were detected in any chicken prior to vaccination, there is good evidence that the chickens used in this work were fully susceptible to infectious coryza. Our use of commercial chickens is a practice that has been adopted by other infectious coryza vaccine researchers (Terzolo et al, 1997;Jacobs et al, 2003;Bragg, 2004;Soriano et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

The vaccination-challenge trial: the gold standard test to evaluate the protective efficacy of infectious coryza vaccines

et al. 2008

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Infectious coryza is an upper respiratory tract disease of chickens with the major impact occurring in multiage flocks. We investigated the relationship between the level of antibodies, as detected by a haemagglutination-inhibition (HI) assay, in infectious coryza-vaccinated chickens and the protection against challenge in those chickens. In one experiment, chickens given a single dose of either of two infectious coryza vaccines lacked a detectable HI response to vaccination but showed significant levels of protection 11 weeks after vaccination. In contrast, in chickens given two doses of an infectious coryza vaccine and challenged 3 weeks after the second vaccine dose, there was a strong serological response with 36/40 birds having a HI titre of 1/20 or greater. In this trial there was an apparent relationship between titre and subsequent protection, with none of the 32 chickens with a titre of 1/40 or 1/80 showing any clinical signs and only one of the same group yielding the challenge organism on culture. In contrast, three of the four vaccinated chickens with a HI titre less than 1/5 developed the typical clinical signs of coryza and yielded the challenge organism on culture. Overall, our results suggest that HI titres cannot be regarded as a definitive predictor of vaccine efficacy. We suggest that the vaccination-challenge trial is the gold standard for the evaluation of the immune response to infectious coryza vaccines.

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Section: Methodsmentioning

confidence: 99%

The vaccination-challenge trial: the gold standard test to evaluate the protective efficacy of infectious coryza vaccines

et al. 2008

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“…1 There is also evidence that, within a Kume serogroup, not all serovars will provide cross-protection. 9 Hence, the limited availability to serotype A. paragallinarum isolates is a major restriction to the effective prevention and control of infectious coryza outbreaks by vaccination.…”

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confidence: 99%

Evaluation of a proposed molecular methodology for the serotyping of Avibacterium paragallinarum

et al. 2016

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A multiplex (m)PCR and a PCR followed by restriction fragment length polymorphism (RFLP) analysis of Avibacterium paragallinarum have been proposed as alternatives to conventional serotyping by the Page scheme. We evaluated both methods, and also sequenced the PCR-RFLP target fragment to reexamine the capacity of molecular serotyping. Eleven reference strains and 27 field isolates were used. Many reference strains and isolates were misidentified as Page serogroup B. The sequence analysis revealed 6 profiles based on the matching rates of the target sequence with the 3 reverse primers of the mPCR. The reference strains and field isolates in profiles 1 and 4 were correctly identified as serogroup A or C by the mPCR. The strains and/or isolates in profiles 2, 3, 5, and 6 could be misidentified as serogroup B or as nontypeable by the mPCR. The homology comparison of the sequences showed that the target sequence of the mPCR, called region 2, was not Page serogroup specific, although some Kume serovars, such as A-1 and C-2, were correctly serotyped. In addition, there was a 9 nucleotide deletion in the sequences of profiles 1, 3, and 5, but not of profiles 2, 4, and 6. Overall, we confirmed that the mPCR and PCR-RFLP molecular assays are not suitable for identifying the serogroups of A. paragallinarum isolates. With further study, analysis of region 2 sequences may have potential as a means of recognizing the Kume serovars of A. paragallinarum isolates.

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“…It is evident that some of the studied Gallibacterium strains have the ability to agglutinate avian or mammal erythrocytes, or both. However, it is unknown if these hemagglutinating antigens play a role in the pathogenicity and immunogenicity of Gallibacterium, as has been previously demonstrated for other pathogenic bacteria in poultry (e.g., Avibacterium paragallinarum) (8).…”

Section: Discussionmentioning

confidence: 95%

Hemagglutinating Activity of Gallibacterium Strains

et al. 2009

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In the present study, the hemagglutinating activity of seven reference strains, and nine Mexican and three Danish field isolates, of Gallibacterium was investigated by using fresh erythrocytes of 19 different types including chicken (broiler, rooster, layer hen), turkey, pigeon, quail, duck, Harris's hawk (Parabuteo unicinctus), house finch (Carpodacus mexicanus), cow, sheep, horse, dog, rabbit, pig, and human (groups A, B, AB, and O; Rh+). Agglutination was observed for broiler chicken, layer hen, quail, rabbit, and pig erythrocytes with a subset of Gallibacterium strains, whereas most tested strains agglutinated rabbit erythrocytes. Transmission electron microscopic examination of a hemagglutinating strain demonstrated a close interaction between the bacterial and erythrocyte surfaces. The results indicate that some Gallibacterium strains are able to agglutinate avian or mammalian erythrocytes, or both. However, the mechanisms enabling hemagglutination are not known and will be addressed in future studies.

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Cross-protection study of the nine serovars ofHaemophilus paragallinarumin the Kume haemagglutinin scheme

Cited by 41 publications

References 19 publications

The vaccination-challenge trial: the gold standard test to evaluate the protective efficacy of infectious coryza vaccines

The vaccination-challenge trial: the gold standard test to evaluate the protective efficacy of infectious coryza vaccines

Evaluation of a proposed molecular methodology for the serotyping of Avibacterium paragallinarum

Hemagglutinating Activity of Gallibacterium Strains

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