Cross-reactions between pneumococci and other streptococci due to C polysaccharide and F antigen

Sørensen, Uffe B. Skov; Henrichsen, Jørgen

doi:10.1128/jcm.25.10.1854-1859.1987

Cited by 64 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All strains were typical pneumococci: gram-positive, lanceolate diplococci often occurring in short chains and growing with typical glistening, dome-shaped colonies showing signs of central autolysis with time. The isolates were optochin sensitive and bile soluble and reacted with anti-C polysaccharide antiserum (11). Their abilities to ferment carbohydrates were consistent with the identification of S. pneumoniae.…”

supporting

confidence: 68%

Six newly recognized types of Streptococcus pneumoniae

Henrichsen¹

1995

J Clin Microbiol

Self Cite

524

148

View full text Add to dashboard Cite

The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. When Streptococcus pneumoniae type 47A was described in 1972 (7), the number of known pneumococcal capsular types totaled 83 (see also reference 6). In 1985, Austrian and coworkers described a new type: 16A (1). In 1979, a worldwide pneumococcal typing surveillance study, aimed mainly at typing isolates from blood and cerebrospinal fluid, was initiated under the auspices of the World Health Organization. Since then, more than 25,000 strains, most from normally sterile body sites, have been typed in Copenhagen, Denmark (2, 9). Typing was performed according to a procedure described previously (6). During the course of this study, six new types, 10B, 10C, 11D, 12B, 25A, and 33D, with which this report deals, were detected by the procedure described by Mørch (8) based on the dictum: ''the serological identity of two types can only be proved by means of crossadsorption of the mutual immune sera'' (4). Antisera to these strains were prepared in two or more rabbits according to the standard immunization procedures of the World Health Organization Reference Centre (6), and antibodies to them are now included in Omniserum, in the diagnostic serum pools D, E, and I, and in antisera to groups 10, 11, 12, 25 (previously type 25), and 33, made at Statens Seruminstitut, Copenhagen, Denmark (3, 6). Antigenic formulas (4), an expression used throughout this paper, represent arbitrary designations of cross-reactions as seen by the capular reaction (4-6). Mørch (8, p. 98) wrote: ''Antigen a signifies the factor characteristic of the individual type or common to the types of a group. The letters b, c, d and so on indicate the additional partial antigens, which in certain cases may have developed more strongly than the antigen particular to the type''. For instance, the two factor sera, 6b and 6c, necessary to distinguish type 6A from type 6B, are of course made by reciprocal absorption which takes experience because there is only a relatively narrow interval between incomplete absorption of the unwanted reaction and complete absorption of all type-specific antibodies, including heterologous antibodies. The reason for including Tables 1 to 5, therefore, is that the antigenic similarities and differences shown represent the only way of demonstrating that the six new types are, in fact, different from the ones to which they are most closely related.

show abstract

supporting

confidence: 68%

Six newly recognized types of Streptococcus pneumoniae

Henrichsen¹

1995

J Clin Microbiol

Self Cite

524

148

View full text Add to dashboard Cite

show abstract

“…The pneumococcal wall lies just under the capsular polysaccharide and consists of two major components, a peptidoglycan polymer, common to all bacterial cells, and teichoic acid, a polysaccharide covalently linked to peptidoglycan. While teichoic acid is found in many gram-positive cell walls, pneumococcal teichoic acid is unique in that it contains phosphorylcholine (15,21). This phosphorus-containing teichoic acid is designated C-polysaccharide and is a recognition site for activation of the alternative complement pathway (10, 30) and the binding of C-reactive proteins (14), certain myeloma proteins (10,22,30), and pneumococcal autolysin (8, 15).…”

Section: Discussionmentioning

confidence: 99%

Role of the bacterial cell wall in middle ear inflammation caused by Streptococcus pneumoniae

Carlsen

Kawana

et al. 1992

Infect Immun

View full text Add to dashboard Cite

The pathogenesis of middle ear inflammation caused by Streptococcus pneumoniae was explored in the chinchilla model with different pneumococcal cell wall (CW) preparations, including isolated native CW, M1 muramidase CW (M1-CW) digest, amidase CW digest, and M1 peptidoglycan (M1-PG) digest. Inflammatory cell and lysozyme concentrations in middle ear fluid (MEF) were measured between 6 and 72 h after the middle ears were inoculated with one of the preparations or sterile saline. Middle ear histopathology was measured quantitatively at 72 h. Native CW, M1-CW digest, and amidase-CW digest caused significantly more inflammatory cell influx and lysozyme accumulation in MEF than saline did. M1-PG digest also caused more inflammatory cell influx and lysozyme accumulation in MEF than saline did but caused less inflammation than native CW or either CW digest. Epithelial metaplasia was significantly greater in ears inoculated with native CW than in ears inoculated with the CW or PG digest or with saline. Pneumococcal CW is, therefore, the principal factor that initiates middle ear inflammation in acute pneumococcal otitis media, and CW teichoication seems to be important in initiating this response.

show abstract

“…Much to our surprise the non-encapsulated S. pneumoniae R 36a and the non-encapsulated E. coli K 12 W300 1 also showed capsule-like material on the surface of the cells. Recent experiments have shown that the positively stained material on the pneumococci consists of so-called C-polysaccharide (14). The loose tufty layers seen on the E. coli cells could represent bundles of pili emanating at their surface and/or other material reacting with Alcian blue-lanthanum nitrate, e.g.…”

Section: Melly Et Al (8) Used the India Ink Techniquementioning

confidence: 99%

Attempts to demonstrate a polysaccharide capsule in Neisseria gonorrhoeae

Reimann

Heise²,

Blom

1988

APMIS

View full text Add to dashboard Cite

Reimann, K., Heise, H. 8t Blom, J. Attempts to demonstrate a polysaccharide capsule in Neisseria gonorrhoeae. APMIS 96: [135][136][137][138][139][140] 1988.The presence or absence of a polysaccharide capsule on the human pathogen Neisseria (N.) gonorrhoeae is still a topic of controversy. For this reason we compared the results obtained by light microscopy (dry India ink-Fuchsin stain) and electron microscopy (Alcian blue-lanthanum nitrate stain) of encapsulated strains of N. meningitidis and Streptococcus (S.) pneumoniae and of non-encapsulated strains of S. pneumoniae and Escherichia (E.) coli with those obtained using the same methods on strains of pilliated and non-pilliated N. gonorrhoeae. After staining with India ink-Fuchsin no capsules could be demonstrated on any of the N. gonorrhoeae strains studied. If present the capsules on these cells are too delicate to be identified by light microscopy. After treatment with Alcian blue-lanthanum nitrate sections of cells of N. meningitidis and S. pneumoniae generally showed the presence of a capsular layer. Sections of cells of the non-encapsulated strain of S. pneumoniae which possess C (common)-polysaccharide also showed surface associated capsule-like material. Similarly the surface of the cells of the E. coli strain showed material which appeared to be tufts of pili and/or M (mucoid)-antigen. In experiments where the N. gonorrhoeae cells were harvested as early as after six hours of growth a capsule-like material was demonstrated on cells of all strains studied.

show abstract

Cross-reactions between pneumococci and other streptococci due to C polysaccharide and F antigen

Cited by 64 publications

References 22 publications

Six newly recognized types of Streptococcus pneumoniae

Six newly recognized types of Streptococcus pneumoniae

Role of the bacterial cell wall in middle ear inflammation caused by Streptococcus pneumoniae

Attempts to demonstrate a polysaccharide capsule in Neisseria gonorrhoeae

Contact Info

Product

Resources

About