Cross Reactions of Hl-a Antibodies

Ej, Yunis; Db, Amos; Sy, Eguro; Dorf, M E

doi:10.1097/00007890-197210000-00012

Cited by 19 publications

(3 citation statements)

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“…The latter has applied for the definition of subtypes of some antigen groups. Finally, adsorption elution assays were used to identify in terms of recognition of different antigens by the same antibody [9][10][11][12]. In the meantime, protein and DNA sequences of different HLA antigens became available, and comparison between the protein sequences and the antibody reactivity was observable [2].…”

Section: Historical Overviewmentioning

confidence: 99%

A Short History of B-Cell HLA Epitopes

Doxiadis,

Loeffler-Wirth,

Lachmann

et al. 2024

Transfus Med Hemother

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Background: HLA epitopes are currently in the focus of transplantation immunogenetics. The main reason is the complexity of the HLA system with >38,000 alleles, the number of which increases steadily. These alleles are determined by the current state-of-the art typing methods like second- and third-generation sequencing. Screening for HLA antibodies is hampered by the lack of specific target beads with all possible alleles described. Summary: A way to circumvent the problem is to define HLA epitopes. The number of antibody-confirmed epitopes, on the other hand, was found to be 72 for HLA class I and 74 for HLA class II. Here, we elaborate on the current knowledge on these HLA epitopes. Absolute definitions of these structures are not yet available. Key Messages: Making use of eplets is a comparable way allowing statistical analyses. However, one should keep in mind that the results obtained are approximative or perhaps better associative. Continuous collaboration is needed for the full understanding of the HLA epitopes. The reactivity toward epitopes remains patient-specific.

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Section: Historical Overviewmentioning

confidence: 99%

A Short History of B-Cell HLA Epitopes

Doxiadis,

Loeffler-Wirth,

Lachmann

et al. 2024

Transfus Med Hemother

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“…This had already been observed by Legrand & Dausset (1972) and others. Yunis et al (1972) noted that their broad anti-HL-AS reacted with some, but not all, thc HL-A1 cells. Mittal & Terasaki (1972) also noted that not all cells possessing the so-called cross-reacting antigen are capable of absorption.…”

Section: Redistribution Of ~~~I~~~~mentioning

confidence: 99%

The Complexity of the HL‐A Gene Product I: Study of a Serum Produced Against HL‐A5 in an HL‐A Semi‐Identical Situation

Legrand

Dausset

1974

Tissue Antigens

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A complex antibody developed in a W18 father immunized by his HL-A5 daughter was analysed. Four different antibodies appeared successively and were isolated by absorption and elution. These antibodies react directly or indirectly (CYNAP) with HL-A5, W21, W5, W17 and W15 cells. Some W21 cells are not reactive ( E T * ) ; some W5 cells are directly reactive, others only in CYNAP, which enables these two categories of cells to be subdivided.By absorption-inhibition, it was possible to demonstrate the existence of several determinants on the gene product, since the saturation of the absorbing cells by one antibody does not prevent the absorption of a second antibody (anti-HL-A5 and anti-[5, W21, W51). These distinct determinants or antigenic factors (Dausset et al. 1965) are shared by several allelic products. Their structures are probably similar since they are cross-reactive. Capping experiments indicate that all these determinants are borne by the same second locus molecule and are independent of the third locus molecule.The hypothesis that these shared and cross-reactive antigenic factors derived from each other by successive duplication and mutation of the corresponding gene is again emphasized.

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“…Recent developments in the study of crossreactions between certain HL-A antigens led us to search for non-immunological methods of studying antisera containing cross-reactive activity. Cross-reactive antisera are defined as exhibiting cytotoxic activity against two o r more antigens in which the reactivity against the second antigen cannot be separated by conventional absorption techniques (Yunis et al 1972). Each crossreacting antigen is capable of absorbing activity against members of its cross-reacting group as well as against itself although the homologous absorption is the most efficienr In the last few years the use of new techni-ques such as blocking of cytotoxicity (Legrand XC Dausset 1973) or absorption of cross-reactive antibodies to platelets followed by elution with pH gradients ) suggests that each oligospecific antiserum consists of a variety of antibodies with slightly different specificities.…”

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confidence: 99%

Isoelectric Focusing of an Anti‐HL‐A2 Antiserum Cross‐Reacting with W28

et al. 1976

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The cytotoxic activity of an anti-HL-A2 antiserum (1-oss-reactive with W28 appeared in three distinct peaks after the serum was subjected t o isoelectric focusing. The pH at which the peaks formed was reproducible using pH 5-8 or pH 3..5 10 gradients. Anti-HLA2 activity occurred in each peak, but the cross-reactive anti-W28 cytotoxicity appeared to be largely confined t o the second and third peaks. The first peak was exclusively Ig<;, the second contained Ig(; and IgM, and the third contained IgC, IgA, and IgM.Received for fiiLblzcrrtzon I Juls. riccuplrd 12 Orfobrr 197.5

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Cross Reactions of Hl-a Antibodies

Cited by 19 publications

References 0 publications

A Short History of B-Cell HLA Epitopes

A Short History of B-Cell HLA Epitopes

The Complexity of the HL‐A Gene Product I: Study of a Serum Produced Against HL‐A5 in an HL‐A Semi‐Identical Situation

Isoelectric Focusing of an Anti‐HL‐A2 Antiserum Cross‐Reacting with W28

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