Cross-reactivities of mammalian MAPKs antibodies in rotifer and copepod: Application in mechanistic studies in aquatic ecotoxicology

Kang, Hye Min; Jeong, Chang Bum; Lee, Young Hwan; Cui, Yan Hong; Kim, Duck-Hyun; Lee, Min Chul; Kim, Hui Su; Han, Jeonghoon; Hwang, Dae Sik; Lee, Su Jae; Lee, Jae Seong

doi:10.1016/j.marpolbul.2016.11.049

Cited by 17 publications

(6 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a prior study, when 0.05, 0.5, & 6 µm polystyrene beads were exposed to rotifers, different sizes of microbeads induced ROS in a size-dependent manner [38]. Besides, the size dependency of microbeads side effects was proved as a consequence of oxidative stress, which is P-JNK, P-p38 activation with increased ROS level [38]. Smaller sized PS-NPs had been shown to induce the activities of ROS and Hif-1α biomarkers.…”

Section: Inflammatory Protein Expression and Oxidative Stressmentioning

confidence: 98%

“…ROS generation by PS-NPs was examined using the ROS ELISA kit showing ROS production was dramatically increased in the PS-NPs 1.5 ppm treated group, indicating that the presence of PS-NPs synergistically aggravated ROS production. In a prior study, when 0.05, 0.5, & 6 µm polystyrene beads were exposed to rotifers, different sizes of microbeads induced ROS in a size-dependent manner [38]. Besides, the size dependency of microbeads side effects was proved as a consequence of oxidative stress, which is P-JNK, P-p38 activation with increased ROS level [38].…”

Section: Inflammatory Protein Expression and Oxidative Stressmentioning

confidence: 99%

“…For the toxicity test, fishes were randomly assigned to control and PS-NPs treated group (n = 20 for each group). For the treatment groups, 70 nm virgin PS-NPs were used and the exposure concentrations were 0.5 ppm, 1.5 ppm, and 5 ppm, which were chosen in accordance with the previous study about the toxicity of NPs on aquatic organisms [38]. The exposure protocol includes acute (~7 days) and chronic (~30 days and~7 weeks) validation for the PS-NPs toxicity.…”

Section: Exposure Of Ps-npsmentioning

confidence: 99%

See 2 more Smart Citations

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Sarasamma

Audira

Siregar

et al. 2020

IJMS

273

View full text Add to dashboard Cite

Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

show abstract

Section: Inflammatory Protein Expression and Oxidative Stressmentioning

confidence: 98%

Section: Inflammatory Protein Expression and Oxidative Stressmentioning

confidence: 99%

Section: Exposure Of Ps-npsmentioning

confidence: 99%

See 1 more Smart Citation

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Sarasamma

Audira

Siregar

et al. 2020

IJMS

273

View full text Add to dashboard Cite

show abstract

“…The MAPK signaling pathway is one of the important pathways in eukaryotic signal transduction. MAPKs are activated by a series of extracellular stimuli, mediating signals from the cell membrane to the nucleus and regulating various physiological processes such as cell proliferation, differentiation, apoptosis, and death ( 49 , 50 ). The Hippo signaling pathway is an evolutionarily conserved pathway that controls organ size from flies to humans.…”

Section: Discussionmentioning

confidence: 99%

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

Ren,

Wang,

Zeng

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

IntroductionThe Kazakh horse, renowned for its excellence as a breed, exhibits distinctive reproductive traits characterized by early maturity and seasonal estrus. While normal reproductive function is crucial for ensuring the breeding and expansion of the Kazakh horse population, a noteworthy decline in reproductive capabilities is observed after reaching 14 years of age.MethodsIn this study, ovarian granulosa cells (GCs) were meticulously collected from Kazakh horses aged 1, 2, 7, and above 15 years old (excluding 15 years old) for whole transcriptome sequencing.ResultsThe analysis identified and selected differentially expressed mRNAs, lncRNAs, miRNAs, and circRNAs for each age group, followed by a thorough examination through GO enrichment analysis. The study uncovered significant variations in the expression profiles of mRNAs, lncRNAs, miRNAs, and circRNAs within GCs at different stages of maturity. Notably, eca-miR-486-3p and miR-486-y exhibited the highest degree of connectivity. Subsequent GO, KEGG, PPI, and ceRNA network analyses elucidated that the differentially expressed target genes actively participate in signaling pathways associated with cell proliferation, apoptosis, and hormonal regulation. These pathways include but are not limited to the MAPK signaling pathway, Hippo signaling pathway, Wnt signaling pathway, Calcium signaling pathway, Aldosterone synthesis and secretion, Cellular senescence, and NF-kappa B signaling pathway—essentially encompassing signal transduction pathways crucial to reproductive processes.DiscussionThis research significantly contributes to unraveling the molecular mechanisms governing follicular development in Kazakh horses. It establishes and preliminarily validates a differential regulatory network involving lncRNA-miRNA-mRNA, intricately associated with processes such as cell proliferation, differentiation, and apoptosis and integral to the developmental intricacies of stromal follicles. The findings of this study provide a solid theoretical foundation for delving deeper into the realm of reproductive aging in Kazakh mares, presenting itself as a pivotal regulatory pathway in the context of horse ovarian development.

show abstract

“…In the process, binding of GnRH to its receptor activates signaling cascades like MAPK, PI3K-Akt [31]. MAPK pathway is essential for cell proliferation and differentiation, survival, death and transformation [32][33]. PI3K-Akt can interact with mTOR pathway to effectively regulate growth hormone in pituitary [34].…”

Section: Discussionmentioning

confidence: 99%

Pineal gland transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in STH sheep with two FecB genotypes

Ren

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: long noncoding RNA (lncRNA) has been identified as important regulator in hypothalamic-pituitary-ovarian axis associated with sheep prolificacy. However, little is known of their expression pattern and potential roles in the pineal gland of sheep. Herein, RNA-Seq was used to detect transcriptome expression pattern in pineal gland between follicular phase (FP) and luteal phase (LP) in FecBBB (MM) and FecB++ (ww) STH sheep, respectively, and differentially expressed (DE) lncRNAs and mRNAs associated with reproduction were identified.Results: Overall, 135 DE lncRNAs and 1,360 DE mRNAs in pineal gland between MM and ww sheep were screened. Wherein, 39 DE lncRNAs and 764 DE mRNAs were identified (FP vs LP) in MM sheep, 96 DE lncRNAs and 596 DE mRNAs were identified (FP vs LP) in ww sheep. Moreover, GO and KEGG enrichment analysis indicated that the targets of DE lncRNAs and DE mRNAs were annotated to multiple biological processes such as phototransduction, circadian rhythm, melanogenesis, GSH metabolism and steroid biosynthesis, which directly or indirectly participate in hormone activities to affect sheep reproductive performance. Additionally, co-expression of lncRNAs-mRNAs and the network construction were performed based on correlation analysis, DE lncRNAs can modulate target genes involved in related pathways to affect sheep fecundity. Specifically, XLOC_466330, XLOC_532771, XLOC_028449 targeting RRM2B and GSTK1, XLOC_391199 targeting STMN1, XLOC_503926 targeting RAG2, XLOC_187711 targeting DLG4 were included.Conclusion: All of these differential lncRNAs and mRNAs expression profiles in pineal gland provide a novel resource for elucidating regulatory mechanism underlying STH sheep prolificacy.

show abstract

Cross-reactivities of mammalian MAPKs antibodies in rotifer and copepod: Application in mechanistic studies in aquatic ecotoxicology

Cited by 17 publications

References 75 publications

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

Pineal gland transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in STH sheep with two FecB genotypes

Contact Info

Product

Resources

About