Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii

Araújo, Anna Érika Vieira de; Conde, Luis Vidal; Silva, Haroldo Cid da; Machado, Lucas de Almeida; Lara, Flávio Alves; Chapeaurouge, Alex; Pauer, Heidi; Hardoim, Cristiane Cassiolato Pires; Antunes, L. Caetano M.; Carvalho-Assef, Ana Paula D’Alincourt; Senna, José Procópio Moreno

doi:10.1016/j.micinf.2021.104801

Cited by 12 publications

(6 citation statements)

References 41 publications

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“…While cross-reactivity may be advantageous for therapeutic purposes [ 44 , 45 ], there is also a great concern of potential detrimental effects on host gut microbiota. A previous study of a vaccine against Acinetobacter baumannii based on recombinant BamA revealed the impact of cross-reactivity on the gut microbiota of animal models [ 46 ]. Nevertheless, Vieira de Araujo et al recently demonstrated that the effect of immunization on the gut microbiota is mild and is likely not the result of cross-reactivity of antibodies against recombinant proteins with homologous proteins in gut bacteria.…”

Section: Discussionmentioning

confidence: 99%

Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae

Chen

Chu

et al. 2022

Vet Res

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In recent years, Klebsiella pneumoniae (KP) has caused disease outbreaks in different animals, resulting in serious economic losses and biosafety concerns. Considering the broad antibiotic resistance of KP, vaccines are the most effective tools against infection. However, there is still no KP vaccine available in the veterinary field. Our results indicate that the highly conserved outer membrane phosphoporin (PhoE) of KP is immunogenic in mice and elicits high titers of antibodies that were shown to be specific for PhoE by immunoblotting. Immunization with PhoE also induced robust cell-mediated immunity and elicited the secretion of high levels of IFN-γ and IL-4, suggesting the induction of mixed Th1 and Th2 responses. Sera from PhoE-immunized mice induced significantly higher complement-mediated lysis of KP cells than did sera from the PBS control mice. Finally, mice immunized with PhoE were significantly protected against KP challenge, with better survival and a reduced visceral bacterial load. Our data underscore the great potential of PhoE as a novel candidate antigen for a vaccine against KP infection.

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Section: Discussionmentioning

confidence: 99%

Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae

Chen

Chu

et al. 2022

Vet Res

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“…Studies in E. coli have demonstrated that bacteria are not viable without this system [ 164 ], likely due to the vital functions of the myriad β-barrel proteins expressed on the outer membrane. The BamA protein has thus been explored recently as a potential vaccine candidate and target of monoclonal antibodies in Acinetobacter baumannii [ 165 , 166 ] and pathogenic E. coli [ 167 , 168 , 169 ].…”

Section: General Outer Membrane Proteins: β-Barrelsmentioning

confidence: 99%

“…In fact, when limiting this analysis of sequence conservation to only the extracellular loops of BamA, Bcc-to- B. pseudomallei sequence identity increases to 98.2%, indicating that such potential antibody targets are almost entirely conserved across pathogenic Burkholderia species ( Figure 2 a,b; Table 3 ). A significant caveat to this is that BamA’s conservation is so high that its use as a vaccine antigen may impact the healthy gut microbiome, as has been reported when mice were immunized with A. baumannii BamA [ 166 ]. Su et al did address this concern to some extent, showing that the B. pseudomallei BamA immunization serum did not cross-react to E. coli [ 27 ], though future studies should expand on this by directly looking at potential changes in the gut microbiome of vaccinated mice.…”

Section: General Outer Membrane Proteins: β-Barrelsmentioning

confidence: 99%

Burkholderia pseudomallei Complex Subunit and Glycoconjugate Vaccines and Their Potential to Elicit Cross-Protection to Burkholderia cepacia Complex

Badten,

Torres

2024

Vaccines

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Burkholderia are a group of Gram-negative bacteria that can cause a variety of diseases in at-risk populations. B. pseudomallei and B. mallei, the etiological agents of melioidosis and glanders, respectively, are the two clinically relevant members of the B. pseudomallei complex (Bpc). The development of vaccines against Bpc species has been accelerated in recent years, resulting in numerous promising subunits and glycoconjugate vaccines incorporating a variety of antigens. However, a second group of pathogenic Burkholderia species exists known as the Burkholderia cepacia complex (Bcc), a group of opportunistic bacteria which tend to affect individuals with weakened immunity or cystic fibrosis. To date, there have been few attempts to develop vaccines to Bcc species. Therefore, the primary goal of this review is to provide a broad overview of the various subunit antigens that have been tested in Bpc species, their protective efficacy, study limitations, and known or suspected mechanisms of protection. Then, we assess the reviewed Bpc antigens for their amino acid sequence conservation to homologous proteins found in Bcc species. We propose that protective Bpc antigens with a high degree of Bpc-to-Bcc sequence conservation could serve as components of a pan-Burkholderia vaccine capable of protecting against both disease-causing groups.

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“…BamA is an outer membrane β-barrel assembly protein responsible for organizing protein complex on the outer membrane of bacteria ( 37 , 90 , 91 ). The formation and assembly of outer membrane proteins on bacterial membranes are related to the action of the Bam protein complex ( 91 ).…”

Section: Candidate Subunit Vaccines For Acinetobacter Bauma...mentioning

confidence: 99%

Subunit vaccines for Acinetobacter baumannii

et al. 2023

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Acinetobacter baumannii is a gram-negative bacterium and a crucial opportunistic pathogen in hospitals. A. baumannii infection has become a challenging problem in clinical practice due to the increasing number of multidrug-resistant strains and their prevalence worldwide. Vaccines are effective tools to prevent and control A. baumannii infection. Many researchers are studying subunit vaccines against A. baumannii. Subunit vaccines have the advantages of high purity, safety, and stability, ease of production, and highly targeted induced immune responses. To date, no A. baumannii subunit vaccine candidate has entered clinical trials. This may be related to the easy degradation of subunit vaccines in vivo and weak immunogenicity. Using adjuvants or delivery vehicles to prepare subunit vaccines can slow down degradation and improve immunogenicity. The common immunization routes include intramuscular injection, subcutaneous injection, intraperitoneal injection and mucosal vaccination. The appropriate immunization method can also enhance the immune effect of subunit vaccines. Therefore, selecting an appropriate adjuvant and immunization method is essential for subunit vaccine research. This review summarizes the past exploration of A. baumannii subunit vaccines, hoping to guide current and future research on these vaccines.

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Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii

Cited by 12 publications

References 41 publications

Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae

Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae

Burkholderia pseudomallei Complex Subunit and Glycoconjugate Vaccines and Their Potential to Elicit Cross-Protection to Burkholderia cepacia Complex

Subunit vaccines for Acinetobacter baumannii

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