Cross-reactivity of anti-Eimeria tenella antibody fragments on merozoites and sporozoites of different chicken Eimeria species

Khalafalla, Reda E.; Daugschies, Arwid; Dyachenko, Viktor

doi:10.1007/s00436-010-2171-9

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…The second-generation merozoites were collected and purified according to the methods reported previously (Khalafalla et al, 2011;Liu et al, 2009). Total RNA was extracted from the second-generation merozoites of E. tenella by the single step protocol previously described (Sambrook et al, 2002).…”

Section: Cloning Of E Tenella 5401 Genementioning

confidence: 99%

“…Firstly, sporozoites of E. tenella were collected and soluble proteins were prepared as described previously (Khalafalla et al, 2011;Liu et al, 2009). Rats were vaccinated subcutaneously on the back with 100 μg proteins emulsified in Freund's Adjuvant Complete (Sigma) at 30 days of age, 14 days later, the second dose was given with Freund's Adjuvant Incomplete (Sigma).…”

Section: Plasmids Parasites Animals and Antiserummentioning

confidence: 99%

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Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Song

Huang

Ren

et al. 2015

Experimental Parasitology

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Section: Cloning Of E Tenella 5401 Genementioning

confidence: 99%

Section: Plasmids Parasites Animals and Antiserummentioning

confidence: 99%

Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Song

Huang

Ren

et al. 2015

Experimental Parasitology

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“…Coccidiosis is an enteric parasitic disease caused by multiple protozoan parasite species of the genus Eimeria and is one of the most common and expensive diseases in the poultry industry (Mehlhorn 2008;Khalafalla et al 2011). It has a major economic impact on both growers and the poultry industry worldwide Metwaly et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Azadirachta indica extract against Eimeria papillata-induced coccidiosis

et al. 2012

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Coccidiosis in poultry is caused by protozoan parasites of the genus Eimeria, which is responsible for worldwide economic losses. The methanolic extract of Azadirachta indica (neem) leaves was used in vivo for its pharmacological, antioxidant, and anticoccidial properties. Four groups of mice were investigated. The first group was inoculated only with sterile saline and served as the control group. The second group was treated by oral gavage with neem extract (500 mg/kg) daily for 4 days. The third and fourth groups were infected with 10(3) sporulated oocysts of Eimeria papillata. The fourth group was also treated once daily with neem extract for 4 days. Paraffin sections from the jejunum as well as jejunal homogenate were prepared for the histopathological and biochemical investigations, respectively. The data showed that mice infected with E. papillata revealed an output of 6.5 × 10(5) ± 29,753 oocysts per gram feces on day 4 postinoculation. This output is significantly decreased to 2.7 × 10(5) ± 37,341 oocysts in neem-treated mice. Infection with E. papillata induced marked histopathological alterations in the jejunum in the form of inflammation, vacuolation of the epithelium, and destruction of some villi. Also, the neem extract greatly diminished body weight loss of infected mice. Moreover, the number of goblet cells stained with Alcian blue within the infected villi was significantly lowered (P ≤ 0.05). In addition, E. papillata enhanced lipid peroxidation and nitric oxide production in both serum and jejunum with concomitant reduction in glutathione. Neem induced marked improvements in all of the studied parameters as well as the histopathological features of the jejunum. Our study revealed that neem as a natural product has protective effects against E. papillata-induced coccidiosis.

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“…Coccidiosis is a common infectious disease in poultry, causing major economic losses (Dkhil et al 2011a;Khalafalla et al 2011). The protozoan parasite of the genus Eimeria multiplies in the intestinal tract of poultry and produces tissue damage, resulting in reduced growth and increased susceptibility to pathogens (McDougald 2003;Metwaly et al 2011).…”

Section: Introductionmentioning

confidence: 99%

The potential role of Phoenix dactylifera on Eimeria papillata-induced infection in mice

2012

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Coccidiosis is a common infectious disease in poultry causing major economic losses. Here, we investigated the effect of Khodary date fruit aqueous extract (4 ml/kg) on the outcome of coccidiosis caused by Eimeria papillata in Swiss Albino mice. Date fruit extract was able to decrease the intracellular development by lowering the faecal output of E. papillata oocysts from 8.7 ± 0.5 × 10(3) to 6.6 ± 0.4 × 10(3) oocysts per gram faeces. Also, date extract caused a great diminish in body weight of infected mice from 19.3 to 3.2 %. The number of parasitic stages in the intestinal villi of the infected mice was reduced to about 52 % after treatment with date extract. The infection was associated with marked histopathological lesions of the murine jejunum in the form of inflammation, vacuolation of the epithelium, and destruction of some villi. Also, the number of goblet cells within the infected villi was significantly lowered (P ≤ 0.05). These changes lead to an oxidative damage of the infected tissue. Moreover, infection induced a disturbance in both protein and carbohydrate content in the infected mice. Treatment of mice with date extract could improve the above-studied parameters. On the basis of the above results it can be hypothesized that date fruit can protect against coccidiosis-induced infection, this hypothesis can be revealed by the anti-inflammatory activity of date protecting host tissue from injuries induced by the parasite, and hence it is recommended to be used as an excellent food additive.

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Cross-reactivity of anti-Eimeria tenella antibody fragments on merozoites and sporozoites of different chicken Eimeria species

Cited by 8 publications

References 29 publications

Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Protective effect of Azadirachta indica extract against Eimeria papillata-induced coccidiosis

The potential role of Phoenix dactylifera on Eimeria papillata-induced infection in mice

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