Cross-reactivity of commercial immunoassays for screening of new amphetamine designer drugs. A review

Nieddu, Maria; Baralla, Elena; Pasciu, Valeria; Rimoli, Maria Grazia; Boatto, Gianpiero

doi:10.1016/j.jpba.2022.114868

Cited by 2 publications

(2 citation statements)

References 65 publications

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“…Interestingly, after evaluating compounds identification in several concentration levels and in two different matrices, the high sensitivity of the proposed method was underlined as the lowest concentration tested, which resulted in the cutoff for most analytes. Different from screening by immunoassays, the proposed method is specific in avoiding false positivity for cross-reactivity mechanisms (Franzin et al 2024;Moody et al 2022;Nieddu et al 2022;Wang et al 2014). Indeed, compounds related to the biological matrix, such as sympathomimetic and putrefactive amines, that usually cross react in immunoassays, do not interfere in the present screening (Bonicelli et al 2022;Broussard 2008;McLaughlin et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous multi-targeted forensic toxicological screening in biological matrices by MRM-IDA-EPI mode

Franzin,

Di Lenardo,

Ruoso

et al. 2024

Arch Toxicol

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The toxicologist ascertains drug assumptions in case of paediatric intoxications and death for overdose. The analytical approach consists of initially screening and consequently confirming drug positivity. We developed a toxicological screening method and validated its use comparing the results with a LC–MS/MS analysis. The method identifies 751 drugs and metabolites (704 in positive and 47 in negative mode). Chromatographic separation was achieved eluting mobile phase A (10 mM ammonium formate) and B (0.05% formic acid in methanol) in gradient on Kinetex Phenyl-Hexyl (50 × 4.6 mm, 2.6 μm) with 0.7 mL/min flow rate for 11 min. Multiple Reaction Monitoring (MRM) was adopted as survey scan and, after an Information-Dependent Analysis (IDA) (threshold of 30,000 for positive and 1000 cps for negative mode), the Enhanced Product Ion (scan range: 50–700 amu) was triggered. The MS/MS spectrum generated was compared with one of the libraries for identification. Data processing was optimised through creation of rules. Sample preparation, mainly consisting of deproteinization and enzymatic hydrolysis, was set up for different matrices (blood, urine, vitreous humor, synovial fluid, cadaveric tissues and larvae). Cut-off for most analytes resulted in the lowest concentration tested. When the results from the screening and LC–MS/MS analysis were compared, an optimal percentage of agreement (100%) was assessed for all matrices. Method applicability was evaluated on real paediatric intoxications and forensic cases. In conclusion, we proposed a multi-targeted, fast, sensitive and specific MRM-IDA-EPI screening having an extensive use in different toxicological fields.

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Section: Discussionmentioning

confidence: 99%

Simultaneous multi-targeted forensic toxicological screening in biological matrices by MRM-IDA-EPI mode

Franzin,

Di Lenardo,

Ruoso

et al. 2024

Arch Toxicol

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“…1-Aryl-2-aminopropanes are widely applied in synthetic chemistry and in the pharmacological field. Also known as amphetamines, they are substances that belong to the psychoanaleptic group, known for their stimulating effects on the sympathetic nervous system as well as for their ability to inhibit various enzymes …”

Section: Introductionmentioning

confidence: 99%

Copper(II)-Catalyzed Three-Component Arylation/Hydroamination Cascade from Allyl Alcohol: Access to 1-Aryl-2-sulfonylamino-propanes

Loro,

Papis,

Foschi

et al. 2023

J. Org. Chem.

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A new straightforward approach to 1-aryl-2-aminopropanes using easily accessible substrates has been developed. Simple allyl alcohol is shown to be an ideal synthetic equivalent of the C3 propane-1,2-diylium bis-cation synthon in threecomponent cascade reactions with arenes and sulfonamide nucleophiles to regioselectively afford 1-aryl-2-aminopropanes. The reaction is catalyzed by Cu(OTf) 2 and is expected to involve a Friedel−Crafts-type allylation of the arene, followed by hydroamination.

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Cross-reactivity of commercial immunoassays for screening of new amphetamine designer drugs. A review

Cited by 2 publications

References 65 publications

Simultaneous multi-targeted forensic toxicological screening in biological matrices by MRM-IDA-EPI mode

Simultaneous multi-targeted forensic toxicological screening in biological matrices by MRM-IDA-EPI mode

Copper(II)-Catalyzed Three-Component Arylation/Hydroamination Cascade from Allyl Alcohol: Access to 1-Aryl-2-sulfonylamino-propanes

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