Cross‐sectional and dynamic change of serum metabolite profiling for Hepatitis B‐related acute‐on‐chronic liver failure by UPLC/MS

Abstract

Summary Acute-on-chronic liver failure(ACLF) is an increasingly recognized entity encompassing an acute deterioration of liver function and results in the failure of one or more organs with high short-term mortality. The focus of this study was to discover noninvasive and reliable biomarkers for the diagnosis and prognosis of hepatitis B-related ACLF. Ultra-performance liquid chromatography-mass spectrometry (UPLC/MS) was used to analyse serum metabolites of 28 patients with hepatitis B-related ACLF, 35 patients with Child-Pugh A cirrhosis, 30 patients with chronic hepatitis B and 35 healthy volunteers (HS). Characteristic metabolites were screened, identified and dynamically tracked to investigate their value for diagnosis and prognosis. After comparing serum metabolic profile of hepatitis B-related ACLF and Child-Pugh A cirrhosis, 99 characteristic metabolites were selected, and 38 of them were identified. Dynamic tracking model demonstrated that 17 metabolites were related to prognosis of hepatitis B-related ACLF, and there were also 11 metabolites which improved with treatment in the survival group. The correlations between these characteristic metabolites and the model for end-stage liver disease score were strong. These observations contributed to the investigation of the mechanisms of hepatitis B-related ACLF manifestation and progression on the metabolic level, and they provided information for the identification of biomarkers for the diagnosis and prognosis of hepatitis B-related ACLF.

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

“…Metabolomic analysis revealed that chenodeoxycholate and glycocholic acid up-regulated the KEGG pathway of primary bile acid biosynthesis in the cecal contents of PEO group, Primary bile acids are also critical to the digestion and absorption of fat 41 . In the blood of chickens of PEO group, six lipid metabolites (such as LysoPC (18:1(9Z)) and LysoPE (16:0/0:0)) enriched fatty acid biosynthesus, Lyso PCs perform essential functions in the lipid metabolism of organisms 9,42 ; 1-Palmitoylglycerol is the biosynthetic precursor of phosphatidic acid: the major component of lysophosphatidic acid, which is a pluripotent lipid mediator which controls growth, motility, and differentiation 43 .…”

1 H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

“…1 H NMR spectra were acquired on a Bruker 400-MHz Avance DRX spectrometer (Bruker Biospin, Germany), operating at 400. 13 MHz with a probe temperature of 300 K. One-dimensional spin echo spectra were acquired using the Carr-PurcellMeiboom-Gill (CPMG) sequence to selectively highlight the signals from low-molecular-weight metabolites by attenuating the signals from macromolecules through a T2 filter. Each plasma spectrum was acquired using 16 dummy scans and 1,024 scans, 32,000 time domain points, a spec-tral width of 20,000 ppm, a relaxation delay of 2 s, an acquisition time of 1.36 s, and a spin echo delay of 400 s. Suppression of the water peak was achieved by water irradiation during the relaxation delay.…”

“…More recently, metabolomics, the study of low-molecular-weight metabolites of physiological relevance, has proved an important tool for studying the changes in host metabolism during the course of viral infections (13)(14)(15)(16)(17). Since the metabolic resources crucial for viral replication are provided by host cells, the detection of changes in the metabolic profile associated with the pathological condition could help with the identification of markers of prognostic and diagnostic importance as well as the development of specific treatment strategies.…”

Acute-on-Chronic Liver Failure

“…A recent study evaluating the metabolomic profile of patients with alcoholic cirrhosis admitted to the hospital with ACLF identified signals related to ACLF, compared to compensated or decompensated cirrhosis, including lactate, pyruvate, ketone bodies, glutamine, phenylalanine, tyrosine, and creatinine 78 . Metabolic profiling has been applied in patients with HBV-related ACLF and 38 characteristic serum metabolites were identified, 17 of which also demonstrated a potential prognostic role 79 . Systemic inflammatory response is an important pathophysiologic feature of ACLF, and therefore, inflammatory or immune markers have also been investigated in this condition.…”