Cross-sectional area of the murine aorta linearly increases with increasing core body temperature

Crouch, A. Colleen; Manders, Adam B.; Cao, Amos A.; Scheven, Ulrich M.; Greve, Joan M.

doi:10.1080/02656736.2017.1396364

Cited by 6 publications

(26 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the relative reduction in area at the infrarenal aorta was greater in young animals. This suggests that mature male mice may be exhibiting deleterious effects based on both sex and age, which is consistent with previously published results from mice and humans …”

Section: Discussionsupporting

confidence: 93%

“…Thus, on average, the vessel is left in an enlarged state and the resulting reduction in strain values demonstrates we are beginning to disrupt the vessel's ability to deform during the cardiac cycle in the same manner as young counterparts. Although other studies have not addressed the relationship between changes in strain and area directly, murine and human data demonstrate a similar relationship between larger area and reduced strain that we observe with dobutamine. Geometric changes have long been a foci with respect to aortic disease .…”

Section: Discussionsupporting

confidence: 75%

“…Novel findings include differences in strain based on location or sex. Combined with previous findings using reactive hyperemia and hyperthermia to challenge the older murine CV system, our body of work clearly illustrates that, like the human condition, healthy mice are not immune to the challenges aging imposes. Therefore, just as sex diversity in preclinical studies has come to the forefront as a critical experimental parameter, so too should the age of mice when modeling CV diseases associated with aging in humans.…”

Section: Discussionsupporting

confidence: 64%

See 2 more Smart Citations

Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion

Castle

Scheven

Crouch

et al. 2018

Magnetic Resonance Imaging

Self Cite

View full text Add to dashboard Cite

Background One of the primary biomechanical factors influencing arterial health is their deformation across the cardiac cycle, or cyclic strain, which is often associated with arterial stiffness. Deleterious changes in the cardiovascular system, e.g., increased arterial stiffness, can remain undetected until the system is challenged, such as under a cardiac stressor like dobutamine. Purpose To quantify cyclic strain in mice at different locations along the arterial tree prior to and during dobutamine infusion, while evaluating the effects of sex and age. Study Type Control/cohort study. Animal Model Twenty C57BL/6 mice; male, female; ∼12 and 24 weeks of age; n = 5 per group. Field Strength/Sequence 7T; CINE MRI with 12 frames, velocity compensation, and prospective cardiac gating. Assessment Prior to and during the infusion of dobutamine, Green–Lagrange circumferential cyclic strain was calculated from perimeter measurements derived from CINE data acquired at the carotid artery, suprarenal and infrarenal abdominal aorta, and iliac artery. Statistical Tests Analysis of variance (ANOVA) followed by post‐hoc tests was used to evaluate the influence of dobutamine, anatomical location, sex, and age. Results Heart rates did not differ between groups prior to or during dobutamine infusion ( P = 0.87 and P = 0.08, respectively). Dobutamine increased cyclic strain in each group. Within a group, increases in strain were similar across arteries. At the suprarenal aorta, strain was reduced in older mice at baseline (young 27.6 > mature 19.3%, P = 0.01) and during dobutamine infusion (young 53.0 > mature 36.2%, P = 0.005). In the infrarenal aorta, the response (dobutamine – baseline) was reduced in older mice (young 21.9 > mature 13.5%, P = 0.04). Data Conclusion Dobutamine infusion increases circumferential cyclic strain throughout the arterial tree of mice. This effect is quantifiable using CINE MRI. The results demonstrate that strain prior to and during dobutamine is influenced by anatomical location, sex, and age. Level of Evidence: 3 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;49:69–80.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 64%

See 1 more Smart Citation

Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion

Castle

Scheven

Crouch

et al. 2018

Magnetic Resonance Imaging

Self Cite

View full text Add to dashboard Cite

show abstract

“…Male mice were chosen in this initial study of artery–vein pairs because this sex showed the smallest response in the aorta in our previous work (male: 0.019 mm 2 /°C vs. female: 0.024 mm 2 /°C, (Crouch et al. )). Mice were anesthetized with 1.25–2% isoflurane in 1 L/min of oxygen (Constantinides et al.…”

Section: Methodsmentioning

confidence: 99%

“…Previous work in the lab quantified cardiac output and the arterial response (area and cyclic strain) to increases in core temperature from head‐to‐toe (Crouch et al. ). However, data regarding changes in the core (alternatively, deep) veins are still missing from this body of work.…”

Section: Introductionmentioning

confidence: 99%

Cross-sectional areas of deep/core veins are smaller at lower core body temperatures

2018

Self Cite

View full text Add to dashboard Cite

The cardiovascular system plays a crucial role in thermoregulation. Deep core veins, due to their large size and role in returning blood to the heart, are an important part of this system. The response of veins to increasing core temperature has not been adequately studied in vivo. Our objective was to noninvasively quantify in C57BL/6 mice the response of artery‐vein pairs to increases in body temperature. Adult male mice were anesthetized and underwent magnetic resonance imaging. Data were acquired from three colocalized vessel pairs (the neck [carotid/jugular], torso [aorta/inferior vena cava (IVC)], periphery [femoral artery/vein]) at core temperatures of 35, 36, 37, and 38°C. Cross‐sectional area increased with increasing temperature for all vessels, excluding the carotid. Average area of the jugular, aorta, femoral artery, and vein linearly increased with temperature (0.10, 0.017, 0.017, and 0.027 mm2/°C, respectively; P < 0.05). On average, the IVC has the largest venous response for area (18.2%/°C, vs. jugular 9.0 and femoral 10.9%/°C). Increases in core temperature from 35 to 38 °C resulted in an increase in contact length between the aorta/IVC of 29.3% (P = 0.007) and between the femoral artery/vein of 28.0% (P = 0.03). Previously unidentified increases in the IVC area due to increasing core temperature are biologically important because they may affect conductive and convective heat transfer. Vascular response to temperature varied based on location and vessel type. Leveraging noninvasive methodology to quantify vascular responses to temperature could be combined with bioheat modeling to improve understanding of thermoregulation.

show abstract

In Vivo MRI Assessment of Blood Flow in Arteries and Veins from Head-to-Toe Across Age and Sex in C57BL/6 Mice

et al. 2019

Self Cite

View full text Add to dashboard Cite

Although widely used as a preclinical model for studying cardiovascular diseases, there is a scarcity of in vivo hemodynamic measurements of the naïve murine system in multiple arterial and venous locations, from head-to-toe, and across sex and age. The purpose of this study is to quantify cardiovascular hemodynamics in mice at different locations along the vascular tree while evaluating the effects of sex and age. Male and female, adult and aged mice were anesthetized and underwent magnetic resonance imaging. Data were acquired from four co-localized vessel pairs (carotid/jugular, suprarenal and infrarenal aorta/inferior vena cava (IVC), femoral artery/vein) at normothermia (core temperature 37 ± 0.2 °C). Influences of age and sex on average velocity differ by location in arteries. Average arterial velocities, when plotted as a function of distance from the heart, decrease nearly linearly from the suprarenal aorta to the femoral artery (adult and aged males: − 0.33 ± 0.13, R 2 = 0.87; − 0.43 ± 0.10, R 2 = 0.95; adult and aged females: − 0.23 ± 0.07, R 2 = 0.91; − 0.23 ± 0.02, R 2 = 0.99). Average velocity of aged males and average volumetric flow of aged males and females tended to be larger compared to adult comparators. With cardiovascular disease as the leading cause of death and with the implications of cardiovascular hemodynamics as important biomarkers for health and disease, this work provides a foundation for sex and age comparisons in pathophysiology by collecting and analyzing hemodynamic data for the healthy murine arterial and venous system from head-to-toe, across sex and age.

show abstract

Cross-sectional area of the murine aorta linearly increases with increasing core body temperature

Cited by 6 publications

References 60 publications

Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion

Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion

Cross-sectional areas of deep/core veins are smaller at lower core body temperatures

In Vivo MRI Assessment of Blood Flow in Arteries and Veins from Head-to-Toe Across Age and Sex in C57BL/6 Mice

Contact Info

Product

Resources

About