Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Figueiredo, Israel; Vericimo, Mauricio Afonso; Cardoso, Luciana Ramos; Clemente, S.C. São; Nascimento, Elmiro Rosendo do; Teixeira, Gerlinde Agate Platais Brasil

doi:10.2478/s11686-013-0157-3

Cited by 11 publications

(14 citation statements)

References 38 publications

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“…Specific anti-Ani s1 and Ani s7 IgE antibodies are detected in patients’ sera after adding diluted Anisakis antigens to ELISA plates, and then incubating with secondary antibodies coupled to an enzyme. After washing, so that excess of unbound antibodies can be removed, a substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal, which is proportional to the antibody-antigen complexes and can be measured as optical density (OD) [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Anisakis sensitization in different population groups and public health impact: A systematic review

et al. 2018

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Anisakis simplex spp. sensitization rates have increased worldwide, with a significant impact on health-care systems. To date, no clear-cut diagnostic criteria and laboratory algorithm have been established, so anisakiasis still represents an under-reported health problem whose clinical manifestations, when present, mimic the much more common allergic and digestive disorders. Aim of the study was to systematically review the available literature on the prevalence of sensitization against Anisakis in the general population and in specific population groups, taking into account the impact of the different available diagnostic techniques on the epidemiological data. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, relevant papers reporting Anisakis sensitization epidemiological data were found covering a period ranging from 1996 to February 2017. Overall, 41 studies comprising 31,701 participants from eleven countries were included in the qualitative synthesis. General asymptomatic population resulted sensitized to Anisakis in 0.4 to 27.4% of cases detected by means of indirect ELISA or ImmunoCAP specific IgE detection, and between 6.6% and 19.6% of the samples by Skin prick test (SPT). Occupationally exposed workers (fishermen, fishmongers and workers of fish-processing industries) documented specific IgE between 11.7% and 50% of cases, whereas SPT positivity ranged between 8% and 46.4%. Symptomatic allergic patients to any kind of allergen were found to be positive to Anisakis specific IgE detection between 0.0% (in children with mastocytosis) to 81.3% (among adults with shellfish allergy). Results highlighted that hypersensitivity prevalence estimates varied widely according to geographical area, characteristics of the population studied, diagnostic criteria and laboratory assays. Further studies are needed to overcome the documented misdiagnosis by improving the diagnostic approach and, consequently, providing more affordable estimates in order to address public health interventions on populations at high risk of exposure to Anisakis and to tailor health services related to specific groups.

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Section: Methodsmentioning

confidence: 99%

Anisakis sensitization in different population groups and public health impact: A systematic review

et al. 2018

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“…In Brazil, this aspect has been little explored. A recent study among volunteers ( FIGUEIREDO JUNIOR et al . 2013 ) showed a link between the frequency of fish consumption and immunoreactivity to Anisakis simplex antigens in healthy adults, suggesting the previous immune sensitization without allergy symptoms.…”

Section: Discussionmentioning

confidence: 99%

MOLECULAR IDENTIFICATION OF Pseudoterranova azarasi LARVAE IN COD (Gadus sp.) SOLD FOR HUMAN CONSUMPTION IN BRAZIL

Marigo

Taniwaki

Pintó

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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Anisakiasis and Pseudoterranovosis are human diseases caused by the ingestion of live Anisakidae larvae in raw, undercooked or lightly marinated fish. Larvae were collected from one salted cod sold for human consumption in a Sao Paulo market in 2013. One section of one brownish larva was used for molecular analyses. The partial COX2 gene sequence from the larva had a nucleotide identity of 99.8 % with Pseudoterranova azarasi, which belongs to the Pseudoterranova decipiens species complex. The risk of allergy when consuming dead larvae in salted fish is not well known and should be considered.

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“…The cutoff was calculated as threefold the average optical densities of 20 wells of the ELISA reaction described, by substituting PBS-T for human sera ( Figueiredo Junior et al, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Principal component analysis of factors for sensitization to Anisakis spp. in postpartum women

Figueiredo¹,

Vericimo²,

Clemente³

et al. 2016

Parasite Epidemiology and Control

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IntroductionImmunoreactivity to Anisakis spp. is believed to be associated with frequency of fish intake. The objective of this study was to evaluate, using principal component analysis, the main factors potentially involved in reactivity to these nematodes in postpartum women.MethodsRetrospective study conducted on a database of 309 postpartum women. All completed a structured questionnaire and had blood samples collected for ELISA analysis of specific immunoglobulins against total Anisakis spp. antigens and assessment of reactivity. Parametric and nonparametric tests were used to assess factors for sensitization in the reactive and nonreactive groups, and a principal component analysis was performed. A Pearson correlation matrix with varimax rotation was used to assess the variables of interest (place of residence, age, number of prenatal visits, type of birth facility, fish intake and frequency, raw fish intake, fish handling, history of allergies).ResultsAfter exclusions, samples from 203 women were assessed. Of these, 52 (25.6%) were reactive for anti-Anisakis IgG. Most women claimed not to handle fish (n = 121) and eat fish only sporadically (n = 71). Significant differences in age were seen between the reactive and nonreactive groups (p = 0.001). The first two components explained 32.55% and 38.94% of variances in the nonreactive and reactive groups respectively. The adjusted matrix assigned greater probabilistic weight to weekly intake frequency (0.804), followed by raw fish intake (0.759), with differences in relation to the nonreactive group.ConclusionCorrelation matrices revealed a direct relationship between seroreactivity to Anisakis spp. and frequency of fish intake in a sample of postpartum women.

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Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Cited by 11 publications

References 38 publications

Anisakis sensitization in different population groups and public health impact: A systematic review

Anisakis sensitization in different population groups and public health impact: A systematic review

MOLECULAR IDENTIFICATION OF Pseudoterranova azarasi LARVAE IN COD (Gadus sp.) SOLD FOR HUMAN CONSUMPTION IN BRAZIL

Principal component analysis of factors for sensitization to Anisakis spp. in postpartum women

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