Cross‐species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator <i>Arabidopsis halleri</i>

Becher, M.; Talke, Ina N.; Krall, Leonard; Krämer, Ute

doi:10.1046/j.1365-313x.2003.01959.x

Cited by 518 publications

(560 citation statements)

References 81 publications

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“…Such a vacuolar sequestration of Zn seems to be driven by a member of the cation diffusion facilitator family (TgMTP1), constitutively-expressed in T. goesingense. Similar constitutively enhanced expression by cation diffusion facilitators has also been observed for T. caerulescens and A. halleri (Assunção et al, 2001;Becher et al, 2004). Lasat & Kochian (2000) proposed a model to explain the higher accumulation of Zn by T. caerulescens compared to T. arvense (Figure 4).…”

Section: Metal Hyperaccumulation and Tolerance In Plantssupporting

confidence: 61%

Phytoextraction: a review on enhanced metal availability and plant accumulation

Nascimento

Xing

2006

Sci. agric. (Piracicaba, Braz.)

208

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Phytoextraction has emerged as a novel approach to clean up metal-polluted soils in which plants are used to transfer toxic metals from soils to shoots. This review provides a synthesis of current knowledge on phytoextraction of metals from soils and their accumulation in plants. The objective is to integrate soil-related (root exudates and chemical amendments) and biological advances to suggest research needs and future directions. As far as can be deduced from the literature, it will be some time before phytoextraction may be established as a commercial technology. For chemically-assisted phytoextraction, research has not shown easily biodegradable compounds to overcome the risks associated with the use of EDTA for poorly available metals in soils. On the other hand, significant progress has been made on the physiological and molecular aspects regarding tolerance and phytoaccumulation of metals in plants. A multidisciplinary approach is warranted to make phytoextraction a feasible commercial technology to remediate metal-polluted soils. Key words: EDTA, phytoremediation, heavy metals, organic acids FITOEXTRAÇÃO: UMA REVISÃO SOBRE DISPONIBILIDADE INDUZIDA E ACUMULAÇÃO DE METAIS EM PLANTASRESUMO: A fitoextração é uma tecnologia emergente para despoluição de solos contaminados por metais pesados que usa plantas para transferir metais do solo para a parte aérea, a qual pode ser removida da área poluída. Esta revisão apresenta uma síntese do atual conhecimento sobre fitoextração de metais pesados do solo e sua acumulação em plantas. O objetivo é integrar em uma mesma discussão os avanços relacionados à química do solo (exsudação radicular e adição de agentes quelantes para aumentar a absorção) e à biologia (tolerância a metais e melhoramento genético) visando sugerir futuras pesquisas na área. Embora promissor, o atual estado de desenvolvimento da fitoextração ainda não permite estabelecê-la como uma tecnologia comercial. A pesquisa ainda não encontrou agentes quelantes facilmente biodegradáveis que possam substituir o EDTA na solubilização de metais pouco disponíveis em solos. Entretanto, significativos progressos têm sido feitos no entendimento dos mecanismos fisiológicos e moleculares de tolerância e acumulação de metais em plantas. Uma abordagem multidisciplinar dos vários aspectos que envolvem a fitoextração poderá tornar essa tecnologia econômica e ambientalmente viável a médio prazo. Palavras-chave: EDTA, fitorremediação, metais pesados, ácidos orgânicos

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Section: Metal Hyperaccumulation and Tolerance In Plantssupporting

confidence: 61%

Phytoextraction: a review on enhanced metal availability and plant accumulation

Nascimento

Xing

2006

Sci. agric. (Piracicaba, Braz.)

208

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“…In both hyperaccumulators, A. halleri and T. caerulescens, the genes ZIP4, ZIP10 and IRT3 are highly expressed in roots under conditions of sufficient Zn supply [44][45][46][51][52][53]. Nevertheless, the expression of these genes remains responsive to changes in Zn supply and decreases with increasing external Zn in both species.…”

Section: Zip Family Of Membrane Transport Proteinsmentioning

confidence: 99%

Transition metal transport

2007

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Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the functions of known transition metal transporters in the context of the unique chemical properties of their substrates. The modifications of the metal homeostasis network associated with the adaptation to an extreme metalliferous environment are illustrated in two Brassicaceae metal hyperaccumulator model plants based on cross-species transcriptomics studies. In a comparison between higher plants and unicellular algae, hypotheses are generated for evolutionary changes in metal transporter complements associated with the transition to multicellularity.

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“…For Zn 2+ only the importer, MTP1, has been described [8,12,13]. It is interesting to note that Arabidopsis halleri, a typical Zn hyperaccumulator, has a constitutive high expression of a MTP1 homologue [14]. In contrast to most essential heavy metals, it is still unclear how Cd 2+ is transported into the vacuole of higher plants.…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of changes in the leaf‐mesophyll tonoplast proteome in dependency of a cadmium exposure of barley (Hordeum vulgare L.) plants

et al. 2009

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Although the vacuole is the most important final store for toxic heavy metals like cadmium (Cd(2+)), our knowledge on how they are transported into the vacuole is still insufficient. It has been suggested that Cd(2+) can be transported as phytochelatin-Cd(2+) by an unknown ABC transporter or in exchange with protons by cation/proton exchanger (CAX) transporters. To unravel the contribution of vacuolar transporters to Cd(2+) detoxification, a quantitative proteomics approach was performed. Highly purified vacuoles were isolated from barley plants grown under minus, low (20 microM), and high (200 microM) Cd(2+ )conditions and protein levels of the obtained tonoplast samples were analyzed using isobaric tag for relative and absolute quantitation (iTRAQ). Although 56 vacuolar transporter proteins were identified, only a few were differentially expressed. Under low-Cd(2+) conditions, an inorganic pyrophosphatase and a gamma-tonoplast intrinsic protein (gamma-TIP) were up-regulated, indicating changes in energization and water fluxes. In addition, the protein ratio of a CAX1a and a natural resistance-associated macrophage protein (NRAMP), responsible for vacuolar Fe(2+) export was increased. CAX1a might play a role in vacuolar Cd(2+) transport. An increase in NRAMP activity leads to a higher cytosolic Fe(2+) concentration, which may prevent the exchange of Fe(2+) by toxic Cd(2+). Additionally, an ABC transporter homolog to AtMRP3 showed up-regulation. Under high Cd(2+) conditions, the plant response was more specific. Only a protein homologous to AtMRP3 that showed already a response under low Cd(2+) conditions, was up-regulated. Interestingly, AtMRP3 is able to partially rescue a Cd(2+)-sensitive yeast mutant. The identified transporters are good candidates for further investigation of their roles in Cd(2+) detoxification. AbstractAlthough the vacuole is the most important final store for toxic heavy metals like cadmium (Cd 2+ ), our knowledge on how they are transported into the vacuole is still scarce. It has been suggested that Cd 2+ can be transported either as phytochelatin-Cd 2+ by an unknown ABC transporter or in exchange with protons by CAX transporters. To unravel the contribution of vacuolar transporters to Cd 2+ detoxification, a quantitative proteomics approach was performed.Highly purified vacuoles were isolated from barley plants grown under minus, low (20 µM), and high (200 µM) Cd 2+ conditions and protein levels of the obtained tonoplast samples were analyzed using iTRAQ TM . Although, 56 vacuolar transporter proteins were identified, however only a few were differentially expressed. Under low-Cd 2+ conditions an inorganic pyrophosphatase and a γ-tonoplast intrinsic protein (γ-TIP) were upregulated, indicating changes in energization and water fluxes. In addition, the protein ratio of a calcium/proton exchanger (CAX1a) and an NRAMP, responsible for vacuolar Fe 2+ export were increased. CAX1a might play a role in vacuolar Cd 2+ transport. An increase in NRAMP activity leads to a higher cytosol...

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Cross‐species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri

Cited by 518 publications

References 81 publications

Phytoextraction: a review on enhanced metal availability and plant accumulation

Phytoextraction: a review on enhanced metal availability and plant accumulation

Transition metal transport

Quantitative detection of changes in the leaf‐mesophyll tonoplast proteome in dependency of a cadmium exposure of barley (Hordeum vulgare L.) plants

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