Cross-species single-cell annotation with orthologous marker gene groups

Abstract

The lack of known cell-type specific marker genes in most plants is a major hurdle for single-cell analysis. To address this challenge, we developed an approach to identify Orthologous Marker Gene groups (OMGs) across monocots and dicots and showed that they are capable of determining cell identities in tomato. This result indicates employing conserved OMGs from reference single-cell maps can annotate cell types in other plant species.