Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Lee, Hsiang-Ting; Huang, Wei‐Yuan

doi:10.5772/intechopen.83556

Cited by 3 publications

(2 citation statements)

References 62 publications

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“…e preparation of cells for organogenic events can be manifested by the accumulation of storage substances, which can be easily converted into energy or be involved in metabolic pathways. is has been suggested in reports concerning callus proliferation and shoot induction (Carciofi et al, 2012;Czernicka et al, 2021;Lee & Huang, 2019;Santos et al, 2018). e strong correlation between starch accumulation and organ development was prominent in many different systems (Fortes & Pais, 2000;Karim et al, 2006;orpe & Murashige, 1968), indicating a gradual gathering before any observable organ formation and further utilization of the storage material in the organogenic process.…”

Section: Discussionmentioning

confidence: 87%

The appearance of amyloplasts as one of the features distinguishing the initial stages of the development of isolated endosperm in kiwiberry

Sala-Cholewa,

Kozieradzka-Kiszkurno,

Malec

et al. 2023

Acta Soc Bot Pol

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The potency of plant cells’ reaction to dedifferentiation and then proliferation corresponds with cell reorganization. The details of cell modifications may rely on the fate of the cells. In this study, the characteristics of an endosperm and specific storage tissue under organogenic and non-organogenic development pathways were analyzed. The experimental system was based on isolated endosperm explants (with the seed coat and embryo removed) of kiwiberry cv. Bingo, placed on organogenic (OCIM) and non-organogenic (NOCIM) callus induction media that differed in the type and concentration of plant growth regulators. Histological and ultrastructural analyses of freshly isolated endosperm and explants afer selected periods of the culture were performed using stereoscopic, bright field, and transmission electron microscopy techniques. The dedifferentiation for both pathways– organogenic and non-organogenic – was similar at the morphological level for the first week of the culture. The histological and ultrastructural analyses revealed limited amyloplasts in the cells on OCIM. e organization of proliferated tissue differed significantly from two weeks of the culture: it was more compact for OCIM and looser for NOCIM. Additionally, at this period, there were more amyloplasts with starch granules in dedifferentiated cells on OCIM. The limited starch granules in proliferated cells on NOCIM were observed only afer four weeks of the culture. The presence of plastids with starch granules depends on the competence of endosperm-derived callus in kiwiberry to organogenic processes and can be an early marker of callus destination.

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Section: Discussionmentioning

confidence: 87%

The appearance of amyloplasts as one of the features distinguishing the initial stages of the development of isolated endosperm in kiwiberry

Sala-Cholewa,

Kozieradzka-Kiszkurno,

Malec

et al. 2023

Acta Soc Bot Pol

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“…In vitro, the combination levels of sodium chloride salt NaCl are necessary to stimulate the vital activities required for growth. The osmotic stress triggers a series of changes of endogenous hormone metabolism, sensing, and signal transduction, which leads to increasing sucrose uptake and starch accumulation and provides sufficient carbon source for further shoot regeneration [16].…”

Section: Mg) (Table-2)mentioning

confidence: 99%

Molecular analysis of Peganum harmala L. callus to determine the gene expression of beta-carboline alkaloids harmine and harmaline

Mutasher

Attiya

2019

eijs

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The external signals are used as elicitors that can modify the levels of secondary metabolites production and trigger the biotechnological enforcements to improve plant production. In this study, sodium chloride NaCl was used as a stimulating factor for the production of beta-carboline alkaloids, harmine and harmaline, in Peganum harmala. The in vitro induced callus of P. harmalain was used as a source for alkaloid production in the stimulating experiments with sodium chloride. The results illustrated that 2 mg.l-1 of NaCl increased the fresh and dry weight of callus with an average of 944.30 and 72.0 mg, respectively. In a comparative analysis through Gas Chromatography (GC), high concentrations of harmine of 58.55 Âµg.g-1 from the root and 56.50Âµg.g-1 from stem callus were recorded upon treatment with 4 mg.l-1 of NaCl. Treatment with 4 mg.l-1 NaCl also showed an increased amount of harmaline concentration in both root and stem, with values of 2.72 and 2.65 Âµg.g-1, respectively. The analysis and calculation of gene expression by real-time PCR of RNA showed that the induced stem callus had a high gene expression with a copy number of 229,030. While in the root, the effect of higher salinity increased the percentage of alkaloids without increasing the copy number of gene expression.

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Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit

et al. 2021

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Key message Macroscopic, ultrastructural, and molecular features—like a ball shape, the presence of starch granules, and the up-regulation of genes involved in carbohydrate metabolism and secondary metabolite biosynthesis—distinguish PT regions within a callus. Abstract The modification of the mass of pluripotent cells into de novo shoot bud regeneration is highly relevant to developmental biology and for agriculture and biotechnology. This study deals with protuberances (PT), structures that appear during the organogenic long-term culturing of callus (OC) in kiwifruit. These ball-shaped regions of callus might be considered the first morphological sign of the subsequent shoot bud development. Sections of PT show the regular arrangement of some cells, especially on the surface, in contrast to the regions of OC beyond the PT. The cells of OC possess chloroplasts; however, starch granules were observed only in PTs’ plastids. Transcriptomic data revealed unique gene expression for each kind of sample: OC, PT, and PT with visible shoot buds (PT–SH). Higher expression of the gene involved in lipid (glycerol-3-phosphate acyltransferase 5 [GPAT5]), carbohydrate (granule-bound starch synthase 1 [GBSS1]), and secondary metabolite (beta-glucosidase 45 [BGL45]) pathways were detected in PT and could be proposed as the markers of these structures. The up-regulation of the regulatory associated protein of TOR (RAPTOR1) was found in PT–SH. The highest expression of the actinidain gene in leaves from two-year-old regenerated plants suggests that the synthesis of this protein takes place in fully developed organs. The findings indicate that PT and PT–SH are specific structures within OC but have more features in common with callus tissue than with organs.

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Cross Talk among Phytohormone Signal and Carbohydrate Metabolism Involving Regenerable Calli Induction under Osmotic Treatment

Cited by 3 publications

References 62 publications

The appearance of amyloplasts as one of the features distinguishing the initial stages of the development of isolated endosperm in kiwiberry

The appearance of amyloplasts as one of the features distinguishing the initial stages of the development of isolated endosperm in kiwiberry

Molecular analysis of Peganum harmala L. callus to determine the gene expression of beta-carboline alkaloids harmine and harmaline

Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit

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