Cross talk between calcium and ROS regulate the UVA‐induced melanin response in human melanocytes

Dumbuya, Hawasatu; Hafez, Salwa Y.; Oancea, Elena

doi:10.1096/fj.201903024r

Cited by 24 publications

(10 citation statements)

References 73 publications

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“…ROS activates the G-protein coupled signaling pathway, which leads to an increase in melanin in human epidermal melanocytes. 43 Many researchers have exploited some natural products to attenuate ROS induced by UVR, and antioxidants in natural products have the potential to stimulate the endogenous capacity in the skin and reduce ROS products. 44 Antioxidant activities in P. cerasoides extracts were analyzed by DPPH and ABTS assay.…”

Section: Discussionmentioning

confidence: 99%

Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of Prunus cerasoides Buch.-Ham. D. Don. Flowers

Kooltheat

Tedasen

Yamasaki

et al. 2023

J Evid Based Complementary Altern Med

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Safe depigmenting agents are currently increasing in the cosmetic or pharmaceutical industry because various compounds have been found to have undesirable side effects. Therefore, the present study aimed to investigate the melanogenesis inhibitory effects of Prunus cerasoides Buch. -Ham. D. Don. flower extracts and their molecular mechanism in B16F10 mouse melanoma cells. Moreover, we also examined phenolic and flavonoid contents, antioxidant activity, chemical constituents of potential extracts, and molecular docking. The highest phenolic and flavonoid contents with the greatest scavenging activity were found in the butanol extract of the P. cerasoides flower compared to other extracts. From all extracts, only crude, diethyl ether, and butanol extracts showed an inhibition of mushroom tyrosinase activity, cellular tyrosinase activity, and melanin content as well as the downregulation of the gene expression of the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) in α-MSH-stimulated B16F10 cells. Based on the molecular docking study, n-hexadecanoic acid, heptadecanoic acid, octadecanoic acid, 9,12-octadecadienoic acid, 9,12,15-octadecanoic acid, and eicosanoic acid might show an inhibitory effect against tyrosinase and MITF. In conclusion, this finding demonstrates that both the diethyl ether and butanol extracts of the P. cerasoides flower can effectively reduce tyrosinase activity and melanin synthesis through the downregulation of the melanogenic gene expression in B16F10 cells and through the molecular docking study. Taken together, the diethyl ether and butanol extracts of the P. cerasoides flower could be an anti-melanogenic ingredient for hyperpigmentary or melasma treatment.

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Section: Discussionmentioning

confidence: 99%

Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of Prunus cerasoides Buch.-Ham. D. Don. Flowers

Kooltheat

Tedasen

Yamasaki

et al. 2023

J Evid Based Complementary Altern Med

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“…Furthermore, calcineurin activity was also inhibited by CEFFE-Cations, resulting in the blocking of NFATc1 nuclear translocation and inhibition of its auto-amplification. Ca 2+ signaling and ROS share a mutually promoting relationship [38]. The CEFFE-Cation group consists of various antioxidant enzymes, not only scavenges ROS in the microenvironment but also promotes endogenous antioxidant enzyme expression, further enhancing the reserve antioxidant capacity of BMMs.…”

Section: Discussionmentioning

confidence: 99%

Fat Extract Modulates Calcium Signaling and Protects Against Hyperactive Osteoclastogenesis in Bone Remodeling with Antioxidant Capacity

Yang¹,

Xu²,

Kan³

et al. 2022

Preprint

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Osteoclasts are cells which are primarily involved in bone remodeling and osteolytic bone diseases. Hyperactive osteoclastogenesis leads to pathological bone loss and microarchitectural deterioration, particularly in postmenopausal osteoporosis. However, given the limitations of current first-line osteoclast inhibitors, there is an urgent need for a novel antiresorptive agent with higher efficiency and fewer side effects. Cell-free fat extract (CEFFE) is the liquid fraction obtained from human adipose tissues, which are enriched with a variety of cytokines and growth factors. This study aims to explore its pharmacologic effect on hyperactive osteoclast formation in vivo and in vitro. CEFFE exhibits excellent potentials to attenuate osteoclast-associated bone loss in an ovariectomy (OVX) mouse model and to inhibit RANKL-induced osteoclastogenesis in primary bone marrow-derived monocytes. Furthermore, the cationic protein fraction of CEFFE (CEFFE-Cation) is identified as the main inhibitory component in osteoclast formation assay. Excessive reactive oxygen species (ROS) production is the main cause of osteoclast overactivation. According to LC-MS/MS analysis, the CEFFE-Cation fraction mainly consists of various antioxidant enzymes, extracellular matrix, and adipokines, which endow it with a superior antioxidant capacity. Calcium signaling contributes to osteoclast maturation. In addition to scavenging ROS, CEFFE-Cation is also capable of mitigating calcium oscillaion, calcineurin activation, and subsequent NFATc1 nuclear translocation during osteoclastogenesis. Overall, this study elucidates the promising translational potential of CEFFE as a next-generation personalized antiresorptive agent for osteolytic bone disease treatment.

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“…PPD may blend with the DT response linked to melanogenesis activation in the following days [ 188 , 189 ]. Recently, a molecular mechanism for UVA-induced melanin, involving a G protein-coupled receptor, a calcium flux increase and the generation of mitochondrial ROS, was described in melanocytes in vitro [ 190 ]. Another potential mechanism for UVA1-induced melanogenesis, especially in repetitive exposure conditions, may involve DNA damage and subsequent p53 accumulation [ 191 ].…”

Section: Effects Of Uva From Sunlightmentioning

confidence: 99%

The Damaging Effects of Long UVA (UVA1) Rays: A Major Challenge to Preserve Skin Health and Integrity

Bernerd

Passeron

Castiel

et al. 2022

IJMS

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Within solar ultraviolet (UV) light, the longest UVA1 wavelengths, with significant and relatively constant levels all year round and large penetration properties, produce effects in all cutaneous layers. Their effects, mediated by numerous endogenous chromophores, primarily involve the generation of reactive oxygen species (ROS). The resulting oxidative stress is the major mode of action of UVA1, responsible for lipid peroxidation, protein carbonylation, DNA lesions and subsequent intracellular signaling cascades. These molecular changes lead to mutations, apoptosis, dermis remodeling, inflammatory reactions and abnormal immune responses. The altered biological functions contribute to clinical consequences such as hyperpigmentation, inflammation, photoimmunosuppression, sun allergies, photoaging and photocancers. Such harmful impacts have also been reported after the use of UVA1 phototherapy or tanning beds. Furthermore, other external aggressors, such as pollutants and visible light (Vis), were shown to induce independent, cumulative and synergistic effects with UVA1 rays. In this review, we synthetize the biological and clinical effects of UVA1 and the complementary effects of UVA1 with pollutants or Vis. The identified deleterious biological impact of UVA1 contributing to clinical consequences, combined with the predominance of UVA1 rays in solar UV radiation, constitute a solid rational for the need for a broad photoprotection, including UVA1 up to 400 nm.

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Cross talk between calcium and ROS regulate the UVA‐induced melanin response in human melanocytes

Cited by 24 publications

References 73 publications

Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of Prunus cerasoides Buch.-Ham. D. Don. Flowers

Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of Prunus cerasoides Buch.-Ham. D. Don. Flowers

Fat Extract Modulates Calcium Signaling and Protects Against Hyperactive Osteoclastogenesis in Bone Remodeling with Antioxidant Capacity

The Damaging Effects of Long UVA (UVA1) Rays: A Major Challenge to Preserve Skin Health and Integrity

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