Cross-talk between Cytosolic Phospholipase A2α (cPLA2α) and Secretory Phospholipase A2 (sPLA2) in Hydrogen Peroxide-induced Arachidonic Acid Release in Murine Mesangial Cells

Han, Won Kon; Sapirstein, Adam; Hung, Cheng Chieh; Alessandrini, Alessandro; Bonventre, Joseph V.

doi:10.1074/jbc.m300424200

Cited by 144 publications

(118 citation statements)

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“…does cPLA 2 -␣ allow sPLA 2 to release fatty acids or vice versa? However, it may be noted that Han et al (15) and Murakami et al (18) showed that in H 2 O 2 -activated mesangial cells and in serum/IL-1␤-stimulated, hGIIA-transfected HEK293 cells, respectively, arachidonate is released in preference to oleate. Because cPLA 2 -␣ displays approximately 10-fold selectivity for phospholipids with an sn-2 arachidonyl chain versus and sn-2 oleoyl chain (1), whereas hGIIA displays essentially no selectivity (29,57), it would appear that in mesangial and HEK293 cells, cPLA 2 -␣ is responsible for most of the arachidonate release in the presence of H 2 O 2 and serum/ IL-␤, respectively.…”

Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 95%

“…Also the fact that the cPLA 2 -␣ inhibitors reduce accumulation of [ 3 H]arachidonate in the medium in the present as well as in previous studies (2,26,28,42) The data presented in this paper provide a particularly clear example of cross-talk between hGIIA or hGX and cPLA 2 -␣. A second clear example of this cross-talk comes from the recent study of Han et al (15). They showed that expression of either hGIIA or group V sPLA 2 in cPLA 2 -␣-deficient murine mesangial cells does not lead to H 2 O 2 -stimulated [ 3 H]arachidonate release, but expression of these sPLA 2 s augments [ 3 H]arachidonate release in mesangial cells that express cPLA 2 -␣ above the fatty acid release seen in cells containing cPLA 2 -␣ but not the sPLA 2 s. Both mitogen-activated protein kinase and protein kinase C are involved in this sPLA 2 -dependent cPLA 2 -␣ activation (15).…”

Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 99%

“…A second clear example of this cross-talk comes from the recent study of Han et al (15). They showed that expression of either hGIIA or group V sPLA 2 in cPLA 2 -␣-deficient murine mesangial cells does not lead to H 2 O 2 -stimulated [ 3 H]arachidonate release, but expression of these sPLA 2 s augments [ 3 H]arachidonate release in mesangial cells that express cPLA 2 -␣ above the fatty acid release seen in cells containing cPLA 2 -␣ but not the sPLA 2 s. Both mitogen-activated protein kinase and protein kinase C are involved in this sPLA 2 -dependent cPLA 2 -␣ activation (15). Other studies have shown that exogenous addition of sPLA 2 to mammalian cells leads to activation of mitogenactivated protein kinases along with activation of cPLA 2 -␣ (for example see Refs.…”

Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 99%

“…Exogenous addition of groups V and X sPLA 2 s to a variety of mammalian cells leads to arachidonate release (7)(8)(9)(10). There is some evidence to suggest that the action of cPLA 2 -␣ is a prerequisite for sPLA 2 function in cells (11,12) and even for the vice versa scenario (13)(14)(15)), but such cross-talk between PLA 2 s remains poorly understood.…”

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Arachidonic Acid Release from Mammalian Cells Transfected with Human Groups IIA and X Secreted Phospholipase A2 Occurs Predominantly during the Secretory Process and with the Involvement of Cytosolic Phospholipase A2-α

Mounier

Ghomashchi

Lindsay

et al. 2004

Journal of Biological Chemistry

137

147

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Stable expression of human groups IIA and X secreted phospholipases A 2 (hGIIA and hGX) in CHO-K1 and HEK293 cells leads to serum-and interleukin-1␤-promoted arachidonate release. Using mutant CHO-K1 cell lines, it is shown that this arachidonate release does not require heparan sulfate proteoglycan-or glycosylphosphatidylinositol-anchored proteins. It is shown that the potent secreted phospholipase A 2 inhibitor Me-Indoxam is cell-impermeable. By use of Me-Indoxam and the cellimpermeable, secreted phospholipase A 2 trapping agent heparin, it is shown that hGIIA liberates free arachidonate prior to secretion from the cell. With hGX-transfected CHO-K1 cells, arachidonate release occurs before and after enzyme secretion, whereas all of the arachidonate release from HEK293 cells occurs prior to enzyme secretion. Immunocytochemical studies by confocal laser and electron microscopies show localization of hGIIA to the cell surface and Golgi compartment. Additional results show that the interleukin-1␤-dependent release of arachidonate is promoted by secreted phospholipase A 2 expression and is completely dependent on cytosolic (group IVA) phospholipase A 2 . These results along with additional data resolve the paradox that efficient arachidonic acid release occurs with hGIIAtransfected cells, and yet exogenously added hGIIA is poorly able to liberate arachidonic acid from mammalian cells.Phospholipases A 2 (PLA 2 s) 1 are a class of enzymes that release fatty acids from the sn-2 position of glycero-phospholipids. Biomedical interest in these enzymes stems from the finding that the liberation of arachidonic acid for the biosynthesis of the eicosanoids (prostaglandins, leukotrienes, and others) is mediated in mammalian cells by one or more PLA 2 s. Current evidence favors a role for cytosolic phospholipase A 2 -␣ (cPLA 2 -␣, also known as group IVA PLA 2 ) as a major component of the arachidonate releasing signal transduction pathway (1-3). Mammals also contain a large number of secreted phospholipases A 2 (sPLA 2 s) (4, 5), and the possible participation of these enzymes in arachidonate release is under active investigation. For example, group V sPLA 2 is present in the macrophage-like cell line p388D1 and contributes a portion of the arachidonate released in response to lipopolysaccharide (6). Exogenous addition of groups V and X sPLA 2 s to a variety of mammalian cells leads to arachidonate release (7-10). There is some evidence to suggest that the action of cPLA 2 -␣ is a prerequisite for sPLA 2 function in cells (11,12) and even for the vice versa scenario (13-15), but such cross-talk between PLA 2 s remains poorly understood.To assess the arachidonate releasing capacity of PLA 2 s in mammalian cells, CHO and HEK293 cell lines that stably express the various enzymes have been established (16 -20). The behavior of human group IIA (hGIIA) and human group X (hGX) sPLA 2 s when transfected in HEK293 and CHO cells has been extensively studied. hGIIA is secreted from HEK293 cells, and most of the extracellular enzyme is a...

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Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 95%

Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 99%

Section: Localization Of Hgiia In Hek293 Cells By Immunofluorescence-mentioning

confidence: 99%

mentioning

confidence: 99%

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Arachidonic Acid Release from Mammalian Cells Transfected with Human Groups IIA and X Secreted Phospholipase A2 Occurs Predominantly during the Secretory Process and with the Involvement of Cytosolic Phospholipase A2-α

Mounier

Ghomashchi

Lindsay

et al. 2004

Journal of Biological Chemistry

137

147

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“…14 PLA2G4A initiates AA release and eicosanoid production, whereas PLA2G2A enhances the production of AA (Figure 2). [15][16][17][18] Mice with the genes for PLA2G4A and PLA2G2A nullified (knocked out) do not produce eicosanoids, have reduced incorporation rates of free AA into cell membranes, yet normal AA levels. 19,20 PLA2G6A cleavage of phospholipids is primarily for the purpose of cellular membrane remodelling, by altering phospholipids/fatty acid ratios and modifying membrane fluidity.…”

Section: Pla2s and Aamentioning

confidence: 99%

The role of phospholipases A2 in schizophrenia

Law¹,

Cotton²,

Berger

2006

Mol Psychiatry

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A range of neurotransmitter systems have been implicated in the pathogenesis of schizophrenia based on the antidopaminergic activities of antipsychotic medications, and chemicals that can induce psychotic-like symptoms, such as ketamine or PCP. Such neurotransmitter systems often mediate their cellular response via G-protein-coupled release of arachidonic acid (AA) via the activation of phospholipases A2 (PLA2s). The interaction of three PLA2s are important for the regulation of the release of AA -phospholipase A2 Group 2 A, phospholipase A2 Group 4A and phospholipase A2 Group 6A. Gene variations of these three key enzymes have been associated with schizophrenia with conflicting results. Preclinical data suggest that the activity of these three enzymes are associated with monoaminergic neurotransmission, and may contribute to the differential efficacy of antipsychotic medications, as well as other biological changes thought to underlie schizophrenia, such as altered neurodevelopment and synaptic remodelling. We review the evidence and discuss the potential roles of these three key enzymes for schizophrenia with particular emphasis on published association studies.

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Platelet‐activating factor‐stimulated production of reactive oxygen species in ovarian granulosa cells from periovulatory follicles

Krüger

Vernunft

et al. 2009

Cytometry Pt A

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The platelet-activating factor (PAF) is a proinflammatory lipid present in the fluid of ovarian Graafian follicle. Ovarian blockage of the PAF receptor (PAFr) reduces ovulations in the rat whereas underlying mechanism is poorly understood. Mural granulosa cells (MGC) were mechanically isolated from the theca interna of bovine periovulatory follicle. The mRNA abundance for PAFr, progesterone receptor and cyclooxygenase-2 were measured by real-time PCR. Cytosolic calcium (Ca 21 ) concentration was assayed by microscopy using Fura-2 AM as indicator, 8-isoprostaglandin F 2a (8-isoPGF 2a ) by an ELISA kit. Fluorescent products arising from intracellular oxidation of hydroethidine (HE) and dihydrorhodamine (DHR) were quantified by flow cytometry. The cells expressed PAFr mRNA and PAFr protein and responded to cPAF (nonhydrolyzable form of PAF) with a pulsating increase in Ca 21 , demonstrating functional PAFr. Elevation of Ca 21 was reversed by WEB-2086, an inverse PAFr agonist. cPAF elevated the level of 8-isoPGF 2a in the medium of MGC cultured with luteinizing hormone (LH). cPAF alone had no significant influence on the oxidation of HE and DHR, or 8-iso-PGF 2a level. In MGC from vital periovulatory follicle, PAF and LH signaling plays an important role in regulating the production of excessive oxidants. Blockage of PAFr seems to interfere with these regulatory processes essential for ovulation. ' 2009 International Society for Advancement of Cytometry

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Cross-talk between Cytosolic Phospholipase A2α (cPLA2α) and Secretory Phospholipase A2 (sPLA2) in Hydrogen Peroxide-induced Arachidonic Acid Release in Murine Mesangial Cells

Cited by 144 publications

References 89 publications

Arachidonic Acid Release from Mammalian Cells Transfected with Human Groups IIA and X Secreted Phospholipase A2 Occurs Predominantly during the Secretory Process and with the Involvement of Cytosolic Phospholipase A2-α

Arachidonic Acid Release from Mammalian Cells Transfected with Human Groups IIA and X Secreted Phospholipase A2 Occurs Predominantly during the Secretory Process and with the Involvement of Cytosolic Phospholipase A2-α

The role of phospholipases A2 in schizophrenia

Platelet‐activating factor‐stimulated production of reactive oxygen species in ovarian granulosa cells from periovulatory follicles

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