Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

Kim, Dae Woo; Kulka, Marianna; Jo, Ara; Eun, Kyoung Mi; Arizmendi, Narcy; Tancowny, Brian P.; Hong, Seung No; Lee, Jung Pyo; Jin, Hong; Lockey, Richard F.; Kim, Dong Kyu; Cho, Seong H.

doi:10.1016/j.jaci.2016.09.026

Cited by 36 publications

(59 citation statements)

References 10 publications

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“…We further hypothesised that decreased signalling by the airway epithelium would decrease inflammatory cell influx into the airway, mitigating asthma pathogenesis. In support of our hypothesis, several studies have shown that cross-talk between epithelial cells and other cells can modulate inflammatory processes related to type 2 immune signalling [15] and IgE-mediated signalling through periostin [16].…”

Section: Introductionsupporting

confidence: 84%

Airway transcriptomic profiling after bronchial thermoplasty

et al. 2019

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BackgroundBronchial thermoplasty is a nonpharmacological, device-based treatment option for a specific population of severe asthmatic subjects, but the underlying mechanisms are largely unknown. The purpose of this study is to identify potential altered pathways by bronchial thermoplasty using a transcriptomic approach.MethodsPatients undergoing bronchial thermoplasty were recruited to the study, and a bronchial brushing sample was obtained before each bronchial thermoplasty session and sent for RNA sequencing. A variance component score test was performed to identify those genes whose expression varied after bronchial thermoplasty sessions. Differential gene expression meta-analysis of severe asthmatic subjects versus controls was performed using public repositories. Overlapping genes were included for downstream pathway and network analyses.Results12 patients were enrolled in our study. A total of 133 severe asthma cases and 107 healthy controls from the public repositories were included in the meta-analysis. Comparison of differentially expressed genes from our study patients with the public repositories identified eight overlapping genes: AMIGO2, CBX7, NR3C2, SETBP1, SHANK2, SNTB1, STXBP1 and ZNF853. Network analysis of these overlapping genes identified pathways associated with neurophysiological processes.ConclusionWe have shown that bronchial thermoplasty treatment alters several gene networks that are important in asthma pathogenesis. These results potentially elucidate the disease-modifying mechanisms of bronchial thermoplasty and provide several targets for further investigation.

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Section: Introductionsupporting

confidence: 84%

Airway transcriptomic profiling after bronchial thermoplasty

et al. 2019

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“…Through interactions with integrins involved in tissue remodeling, it plays a role in airway subepithelial fibrosis . Periostin expression levels are especially high in patients with active disease, and decrease after effective treatment . Another of the most highly expressed proteins in our study, CST1, was investigated by Kato et al., showing that stimulation with CST1 increases expression of CCL11 and POSTN in nasal fibroblasts.…”

Section: Discussionsupporting

confidence: 47%

Translating transcription: proteomics in chronic rhinosinusitis with nasal polyps reveals significant discordance with messenger RNA expression

Workman

Nocera

Müller

et al. 2019

Int Forum Allergy Rhinol

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Background: Much of the literature examining chronic rhinosinusitis with nasal polyps (CRSwNP) immunopathology has been predicated on messenger RNA (mRNA) analysis with the assumption that transcriptional changes would reflect end-effector protein expression. The purpose of this study was to test this hypothesis using matched transcriptomic and proteomic data sets. Methods:Matched tissue proteomic and transcriptomic arrays were quantified in CRSwNP polyp tissue and control inferior turbinate tissue (n = 10/group). Mucus samples were additionally collected in 6 subjects from each group. Proteins were grouped into functional categories by bioinformatics and differential expression analyses. Log-log regression and Pearson correlations were performed to determine the level of agreement between data sets. Results:Of the 1310 proteins examined, 393 were significantly differentially expressed in CRSwNP. On regression analysis, differences in protein expression were poorly predicted by differences in mRNA expression (R 2 = 0.020, p < 0.05). Several genes canonically thought to be overexpressed in CRSwNP, including IL-5, IL-13, TSLP, CCL13, and CCL26, showed substantial increases in mRNA transcription, but had minimally or unchanged protein expres-sion. Others, including IgE, periostin, CCL18, and CST1/2, were increased at both the transcriptomic and proteomic levels. Among differentially regulated proteins, tissue and mucus protein levels showed weak correlation (r = 0.26, p < 0.0001).Conclusion: Proteomic analysis in CRSwNP has revealed novel disease-associated proteins and pathways, yet correlates poorly with transcriptomic data. The increasing availability of proteomic arrays opens the door to new potential explanatory mechanisms in CRSwNP and suggests that mRNA based studies should be validated with protein analysis. C 2019 ARS-AAOA, LLC.

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“…OSMF is a premalignant mucosal disease with inflammation and progressive fibrosis. As an inducer of fibrosis in various tissues, periostin might mediate the fibrosis process of OSMF (25), and the potential regulatory roles of periostin in mast cells of OLP deserve further investigation (26).…”

Section: Discussionmentioning

confidence: 99%

Expression and clinical significance of periostin in oral lichen planus

Zhang

Chen

et al. 2018

Exp Ther Med

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Oral lichen planus (OLP) is a chronic inflammatory lesion involving the oral mucosa, which has a high likelihood of progressing to cancer. The present study investigated the periostin expression in the mucosa and serum of patients with OLP and its correlation with serum cytokines. A total of 117 patients with OLP and 110 healthy controls were included in the study. The protein expression of periostin were measured in the OLP and normal oral mucosa by immunohistochemistry. ELISA was performed to measure the serum levels of periostin, tumour necrosis factor-α (TNF-α), interleukin (IL)-6, interferon-γ (IFN-γ), IL-4 and thymic stromal lymphopoietin (TSLP). Compared with the control group the OLP group had a significantly increased number of cases with high periostin expression in the oral mucosa and a significantly increased serum periostin level (P<0.05). Among all 117 OLP subjects, high periostin expression was associated with higher serum IL-6, TNF-α, TSLP and tissue mast cell density. High periostin expression was also significantly associated with a lower IFN-γ/IL-4 ratio (P<0.05). The present study concluded that periostin expression was increased in the oral mucosa and serum of patients with OLP and was associated with inflammatory response, T helper 2 cytokine-predominant immune imbalance, increased mast cell count and TSLP. The modulation of periostin may represent an attractive novel therapeutic target.

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Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps

Cited by 36 publications

References 10 publications

Airway transcriptomic profiling after bronchial thermoplasty

Airway transcriptomic profiling after bronchial thermoplasty

Translating transcription: proteomics in chronic rhinosinusitis with nasal polyps reveals significant discordance with messenger RNA expression

Expression and clinical significance of periostin in oral lichen planus

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