The use of deep learning techniques for White Blood Cell (WBC) classification has garnered significant attention on medical image analysis due to its potential to automate and enhance the accuracy of WBC classification, which is critical for disease diagnosis and infection detection. Convolutional neural networks (CNNs) have revolutionized image analysis tasks, including WBC classification effectively capturing intricate spatial patterns and distinguishing between different cell types. A key advantage of deep learning-based WBC classification is its capability to handle large datasets, enabling models to learn the diverse variations and characteristics of different cell types. This facilitates robust generalization and accurate classification of previously unseen samples. In this paper, a novel approach called Red Deer Optimization with Deep Learning for Robust White Blood Cell Detection and Classification was presented. The proposed model incorporates various components to improve performance and robustness. Image pre-processing involves the utilization of median filtering, while U-Net++ is employed for segmentation, facilitating accurate delineation of WBCs. Feature extraction is performed using the Xception model, which effectively captures informative representations of the WBCs. For classification, BiGRU model is employed, leveraging its ability to model temporal dependencies in the WBC sequences. To optimize the performance of the BiGRU model, the RDO is utilized for parameter tuning, resulting in enhanced accuracy and faster convergence of the deep learning models. The integration of RDO contributes to more reliable detection and classification of WBCs, further improving the overall performance and robustness of the approach. Experimental results demonstrate the superiority of our Red Deer Optimization with deep learning-based approach over traditional methods and standalone deep learning models in achieving robust WBC detection and classification. This research highlights the possibility of combining deep learning techniques with optimization algorithms for improving WBC analysis, offering valuable insights for medical professionals and medical image analysis.