Crossability Studies of Interspecific Hybridization among Vigna Species

Abstract

A total of 80 interspecific crosses i.e., 36 each of V. radiata × V. umbellata (V. radiata as female) and V. mungo × V. umbellata (V. mungo as female) and 08 crosses of V. radiata × V. mungo (V. radiata as female) were attempted to study the crossability relationship among these three Vigna species. Among the crosses of V. radiata × V. umbellata the crossability was observed highest in HUM 12 × RBL 9 (16.27%) followed by HUM 12 × RBL 9 (15.78%). In case of V. mungo × V. umbellata, the maximum crossability of 11.36% was noticed in cross, Mash 338 × RBL 9. For V. radiata × V. mungo, the highest crossability was visualized in hybrid, ML 1464 × Mash 338 (37.5%). The study indicated that different kinds of pre and post fertilization barriers are responsible for complete sterility to low fertility. RBL 1 and RBL 9 gnotypes of ricebean showing substantially high percent of crossability and better seed set with different cultivars of mungbean and blackgram may be utilized for genetic improvement of the mungbean and blackgram.

Alien Introgression Studies Involving Vigna mungo x V. umbellate Hybridization

“…In Vigna crops, inability of pollen tube to germinate and penetrate stigma and style (Chowdhury and Chowdhury, 1977) and ovary (Gopinathan et al, 1986), slow rate of pollen growth (Thiyagu et al, 2008); are reported to be significant pre-fertilization barriers. Post-fertilization barriers of varying degrees have been reported in most of the inter-specific Vigna crosses (Gopinathan et al, 1986;Bharithi et al, 2006;Pandiyan et al, 2010;Chaisan et al, 2013;Basavaraja et al, 2018;Bhanu et al, 2018). Thus, recovery of desirable recombinants is reduced, as hybrids exhibit varying levels of sterility (Rashid et al, 2013), inviability, lethality and genotype specific response (Dhiman et al, 2013).…”

Genetics, genomics, and breeding of black gram [Vigna mungo (L.) Hepper]

“…The cross-compatibility study of V. mungo with other species revealed 37.5% compatibility between V. radiata (♀) and V. mungo (♂), whereas it was 11.6% between V. mungo (♀) and Vigna umbellata (♂) ( Bhanu, 2018 ). The true interspecific F 1 ’s among V. radiata (♀, SML 668 and SML 832) and V. mungo (♂, Mash 114 and Mash 218) reported 5.5% to 24.1% pod set, 14.29% to 30.56% germination, and 22.59% to 28.36% pollen fertility ( Lekhi et al., 2018 ).…”

“…The true interspecific F 1 ’s among V. radiata (♀, SML 668 and SML 832) and V. mungo (♂, Mash 114 and Mash 218) reported 5.5% to 24.1% pod set, 14.29% to 30.56% germination, and 22.59% to 28.36% pollen fertility ( Lekhi et al., 2018 ). Different kinds of pre- and post-fertilization barriers were responsible for complete sterility or low fertility in F 1 hybrids between these two species ( Bhanu, 2018 ). Rice bean ( V. umbellata ) genotypes RBL 1 and RBL 9 showed high crossability and percent seed set with different cultivars of black gram.…”

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

“…Interspecific incompatibility in Vigna species is influenced by the parental genotype and meiotic irregularities (6). It was reported that the crossability of interspecific hybrids among Vigna radiata, Vigna mungo and Vigna umbellata and identified fertilization barriers in some crosses, which resulted to embryo degeneration and abnormal seed development (11). They further identified incompatibility barriers among self-and cross pollinated Vigna species such as delayed entry of pollen tube to the ovules and delayed and failure in nuclear division of endosperm.…”