Crossed digoxin immunoreactivity in chromatographic fractions of rat adrenal extract

Schreiber, V; Stĕpán, J; Gregorová, I; Krejčíková, J.

doi:10.1016/0006-2952(81)90170-2

Cited by 17 publications

(4 citation statements)

References 2 publications

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“…Recently, several reports have appeared suggesting the presence of endogenous digitalis-like substances in various organs or body fluids (5)(6)(7)(8)(9)(10)(11)(12)(13). Besides the presence of DLIS, DTLIS was detected in amniotic fluid or cord blood in our study.…”

Section: Discussionsupporting

confidence: 58%

Digoxin- and Digitoxin-Like Immunoreactive Substances in Amniotic Fluid, Cord Blood, and Serum of Neonates

et al. 1986

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ABSTRAm. Using four different digoxin kits, it was disclosed that the majority of various samples including amniotic fluid, cord blood, and serum from neonates contained substantial levels of digoxin-like immunoreactive substance. The differences in data seemed to be due to the range of epitopes which are recognized by antidigoxin antiserum. The day-to-day studies on sera serially obtained from infants at birth to 48 days old revealed that the level of the substance (0.31 f 0.12 ng/ml) in sera of the l-dayold neonates rapidly declined to the level of 0.1 ng/ml by the 2nd postnatal wk and thereafter gradually declined. The immunological specificity and accuracy of the detection of digoxin-like immunoreactive substance was confirmed by a sample dilution test, a recovery test for standard digoxin, and an absorption test with antidigoxin antiserum. The amniotic fluid and cord blood also contained Recent reports (1-3) renewed our attention toward the falsepositive digoxin measurements in the plasma of premature infants (4). From the clinical viewpoint, these observations raise some important questions. The first question is whether the falsepositive digoxin measurement really indicates the presence of a DLIS which leads to an erroneous judgment about the level of digoxin in monitoring of neonatal samples. The second question is whether there is any similarity in biochemical specificity between DLIS and the so-called "ouabain-like substances" detected in other organs or body fluids (5-13). It seems that previous reports (1-3) have not yet provided substantial evidence for their conclusion that the substance is not a product of technical or nonimmunological false positivity. Therefore, in this communication, we tried to confirm the previous findings using Received September 6, 1984; accepted August 19, 1985. Address for correspondence Hiroyoshi Ebara, M.D., Department of Pediatrics, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi-shi, Gunma-ken, Japan. amniotic fluid, cord blood, and serum specimen from neonates and also to evaluate immunological specificity of the reaction and presence of a DTLIS. MATERIALS AND METHODSThirty-one amniotic fluid and 53 cord blood samples were obtained from mothers who had not received any digitalis glycosides during pregnancy and labor. One hundred eleven sera were also obtained from 23 neonates over 35-wk gestational age who were delivered from healthy mothers who had not been on digitalis therapy. The mean and range of birth weight of the neonates were 3264 g and 20 16-4420 g, respectively. The investigation was approved by the ethical committee of the hospital and informed parental consent was obtained for blood sampling. All samples were stored at -20" C in separate small quantities to avoid unnecessary freezing and thawing, and analyzed at various intervals, always within 30 days of collection.For the detection of DLIS, one EIA kit and three RIA kits available in Japan were used: kit A (Markit Digoxin, DainipponSeiyaku, Osaka, Japan); kit B (Amerlex Digoxin RIA k...

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Section: Discussionsupporting

confidence: 58%

Digoxin- and Digitoxin-Like Immunoreactive Substances in Amniotic Fluid, Cord Blood, and Serum of Neonates

et al. 1986

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“…Based on the hypothesis that CTS have a steroidal structure, Schreiber et al (1981b), demonstrated that rat adrenal extracts contain a digitalis-like immunoreactive material. Since that time, evidence for the adrenocortical origin of CTS has grown.…”

Section: Salt-sensitive Hypertension Concept Of Natriuretic Hormmentioning

confidence: 99%

Endogenous Cardiotonic Steroids: Physiology, Pharmacology, and Novel Therapeutic Targets

Shapiro²,

2009

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“…Third, inhibition of the ouabain-sensitive forms of NKA occurs in the range of low nanomolar concentrations in humans, thus, if the endogenous ligand has a similar or greater affinity, circulating levels would be very low. Fourth, there is disagreement as to the material's tissue of origin-the adrenal (49)(50)(51)(52)(53)(54)(55)(56) and hypothalamus (57)(58)(59)(60)(61)(62)(63) being the front runners. Finally, there is no single assay to identify this material.…”

Section: The Endogenous Cardiac Glycosidementioning

confidence: 99%

Regulation of Na,K-ATPase by Endogenous Ouabain-like Materials

Doris

1994

Experimental Biology and Medicine

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Sodium, potassium-ATPase (NKA) is an essential energy transduction mechanism in which the free energy released by hydrolysis of ATP is transferred to an electrochemical gradient across the cell membrane. The asymmetry of sodium in this gradient is coupled to membrane transport mechanisms which affect transmembrane movement of a range of solutes and electrolytes. Recent advances in the molecular biology of NKA have revealed important new aspects of structure-function relationships as well as illuminating the basis for variations in cardiac glycoside sensitivity of the enzyme. The search for endogenous mammalian counterparts of the cardiac glycosides, which regulate the activity of the enzyme by interacting from the extracellular surface at this receptor site, has moved ahead dramatically with evidence that ouabain is an endogenous product of the mammalian adrenal cortex. These advances, and problems raised by them, are explored in this review.

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Crossed digoxin immunoreactivity in chromatographic fractions of rat adrenal extract

Cited by 17 publications

References 2 publications

Digoxin- and Digitoxin-Like Immunoreactive Substances in Amniotic Fluid, Cord Blood, and Serum of Neonates

Digoxin- and Digitoxin-Like Immunoreactive Substances in Amniotic Fluid, Cord Blood, and Serum of Neonates

Endogenous Cardiotonic Steroids: Physiology, Pharmacology, and Novel Therapeutic Targets

Regulation of Na,K-ATPase by Endogenous Ouabain-like Materials

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