Crosses between Saccharomyces cerevisiae and Saccharomyces bayanus generate fertile hybrids

Sebastiani, Federico; Barberio, Claudia; Casalone, Enrico; Cavalieri, Duccio; Polsinelli, Mario

doi:10.1016/s0923-2508(01)01286-4

Cited by 54 publications

(82 citation statements)

References 23 publications

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“…In these cases, experimental inactivation of the MAT locus in diploids was used to construct allotetraploids that proved meiotically fertile. But spontaneous endomitotic events can also produce fertile allotetraploids from sterile allodiploids (192). Note the similarity of this process with the natural occurrence of autotetraploids in domesticated S. cerevisiae strains (2) and with the whole-genome duplication in the ancestry of Saccharomyces and related yeasts (230).…”

Section: Meiotic Fertility Of Hybrid Genomes and Hybrid Speciation Emmentioning

confidence: 84%

“…This method has been applied successfully to produce a variety of interspecific hybrids between Saccharomyces sensu stricto species (see Table S2 in the supplemental material) in order to study speciation, epistasis, genetic compatibility, and stability of hybrid genomes (70,192) or simply to produce new strains with novel physiological properties (5,13,99,193). Mass mating was also applied successfully to produce hybrids between more distantly related yeasts (131).…”

Section: Interspecific Matingmentioning

confidence: 99%

“…(2) Hybrid speciation can be obtained by mating-type switching in the haploid clones issued from live ascospores, followed by the formation of homozygous diploids (71). (3) Endoreplication transforms an unfertile diploid hybrid (H 2n) into a meiotically fertile allotetraploid (H 4n) that is able to give rise to diploid ascospores (192). (4) The same is true for allotetraploids formed directly by mating between diploids of distinct species (70). allodiploid hybrids obtained between different Saccharomyces sensu stricto species (70,198).…”

Section: Meiotic Fertility Of Hybrid Genomes and Hybrid Speciation Emmentioning

confidence: 99%

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Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Morales

Dujon

2012

Microbiol Mol Biol Rev

155

158

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SUMMARYForced interspecific hybridization has been used in yeasts for many years to study speciation or to construct artificial strains with novel fermentative and metabolic properties. Recent genome analyses indicate that natural hybrids are also generated spontaneously between yeasts belonging to distinct species, creating lineages with novel phenotypes, varied genetic stability, or altered virulence in the case of pathogens. Large segmental introgressions from evolutionarily distant species are also visible in some yeast genomes, suggesting that interspecific genetic exchanges occur during evolution. The origin of this phenomenon remains unclear, but it is likely based on weak prezygotic barriers, limited Dobzhansky-Muller (DM) incompatibilities, and rapid clonal expansions. Newly formed interspecies hybrids suffer rapid changes in the genetic contribution of each parent, including chromosome loss or aneuploidy, translocations, and loss of heterozygosity, that, except in a few recently studied cases, remain to be characterized more precisely at the genomic level by use of modern technologies. We review here known cases of natural or artificially formed interspecies hybrids between yeasts and discuss their potential importance in terms of genome evolution. Problems of meiotic fertility, ploidy constraint, gene and gene product compatibility, and nucleomitochondrial interactions are discussed and placed in the context of other known mechanisms of yeast genome evolution as a model for eukaryotes.

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Section: Meiotic Fertility Of Hybrid Genomes and Hybrid Speciation Emmentioning

confidence: 84%

Section: Interspecific Matingmentioning

confidence: 99%

Section: Meiotic Fertility Of Hybrid Genomes and Hybrid Speciation Emmentioning

confidence: 99%

See 1 more Smart Citation

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Morales

Dujon

2012

Microbiol Mol Biol Rev

155

158

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“…wasps were fed 10 8 cells of BY4742-GFP/FOX3 (Mat α his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0). S. cerevisiae isolates were identified by sequencing the ribosomal intergenic region as described by Sebastiani et al (45). S. cerevisiae isolates were then grown in rich medium supplemented with 0.2% oleate.…”

Section: Methodsmentioning

confidence: 99%

Role of social wasps in Saccharomyces cerevisiae ecology and evolution

Stefanini

Dapporto

Legras

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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267

236

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Saccharomyces cerevisiae is one of the most important model organisms and has been a valuable asset to human civilization. However, despite its extensive use in the last 9,000 y, the existence of a seasonal cycle outside human-made environments has not yet been described. We demonstrate the role of social wasps as vector and natural reservoir of S. cerevisiae during all seasons. We provide experimental evidence that queens of social wasps overwintering as adults (Vespa crabro and Polistes spp.) can harbor yeast cells from autumn to spring and transmit them to their progeny. This result is mirrored by field surveys of the genetic variability of natural strains of yeast. Microsatellites and sequences of a selected set of loci able to recapitulate the yeast strain's evolutionary history were used to compare 17 environmental wasp isolates with a collection of strains from grapes from the same region and more than 230 strains representing worldwide yeast variation. The wasp isolates fall into subclusters representing the overall ecological and industrial yeast diversity of their geographic origin. Our findings indicate that wasps are a key environmental niche for the evolution of natural S. cerevisiae populations, the dispersion of yeast cells in the environment, and the maintenance of their diversity. The close relatedness of several wasp isolates with grape and wine isolates reflects the crucial role of human activities on yeast population structure, through clonal expansion and selection of specific strains during the biotransformation of fermented foods, followed by dispersal mediated by insects and other animals. evolutionary biology | genomics

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“…In addition to A. gossypii, we used other two Saccharomyces species to re-enforce our argument. These two species' genomic features are very similar to those of S. cerevisiae (Naumov et al, 1992) and they even can cross with each other to generate fertile allotetraploid hybrids (Sebastiani et al, 2002).…”

mentioning

confidence: 93%

Verification of a new gene on Saccharomyces cerevisiae chromosome III

Dietrich

2003

Yeast

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We present verification of the existence of a previously unannotated, introncontaining gene of 134 amino acids (predicted molecular weight ∼15.5 kDa) located on chromosome III of Saccharomyces cerevisiae. Strains carrying a deletion of this gene, which we have called YCL012C, reveal no apparent phenotype. Orthologues of YCL012C are present in related species S. bayanus, S. paradoxus and Ashbya gossypii.Comparison with other fungal sequences reveals that orthologues of this gene are likely present in Schizosaccharomyces pombe, Neurospora crassa and Cryptococcus neoformans as well, indicating that YCL012C is a widely conserved fungal gene.

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Crosses between Saccharomyces cerevisiae and Saccharomyces bayanus generate fertile hybrids

Cited by 54 publications

References 23 publications

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Role of social wasps in Saccharomyces cerevisiae ecology and evolution

Verification of a new gene on Saccharomyces cerevisiae chromosome III

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