Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Liu, Yang; Li, Ren; Wang, Yingjun

doi:10.1016/j.msec.2012.08.030

Cited by 73 publications

(53 citation statements)

References 23 publications

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“…After cross‐linking, the homogenous mixture was dispensed into a specific mold made of polypropylene (PP). The membranes were air dried at 20°C for a week, air humidity 70%, and then rinsed three times with deionized water …”

Section: Methodsmentioning

confidence: 99%

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Long

Liu

et al. 2014

J Biomedical Materials Res

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Although collagen with outstanding biocompatibility has promising application in corneal tissue engineering, the mechanical properties of collagen-based scaffolds, especially suture retention strength, must be further improved to satisfy the requirements of clinical applications. This article describes a toughness reinforced collagen-based membrane using silk fibroin. The collagen-silk fibroin membranes based on collagen [silk fibroin (w/w) ratios of 100:5, 100:10, and 100:20] were prepared by using silk fibroin and cross-linking by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. These membranes were analyzed by scanning electron microscopy and their optical property, and NaCl and tryptophan diffusivity had been tested. The water content was found to be dependent on the content of silk fibroin, and CS10 membrane (loading 10 wt % of silk fibroin) performed the optimal mechanical properties. Also the suture experiments have proved CS10 has high suture retention strength, which can be sutured in rabbit eyes integrally. Moreover, the composite membrane proved good biocompatibility for the proliferation of human corneal epithelial cells in vitro. Lamellar keratoplasty shows that CS10 membrane promoted complete epithelialization in 35 ± 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization, and keratoconus are not observed. The composite films show potential for use in the field of corneal tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Long

Liu

et al. 2014

J Biomedical Materials Res

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“…The diffusion coefficient of the hydrogels was measured as previously described [12]. Ion diffusion and tryptophan permeability of the films were carried out at 35°C using a device with two-compartment chambers.…”

Section: Diffusion Coefficient Assaymentioning

confidence: 99%

“…Collagen protein which is used widely as a biomaterial is a biological macromolecule, with perfect biocompatibility, biodegradability, and biological activity [9][10][11]. Recently, collagen-based films were developed as corneal repair materials and the materials possess outstanding physical, chemical, and biological properties [12,13]. But collagen films have low mechanical strength which is inconvenient in clinic such as hard to suture when operating and nursing after operation.…”

Section: Introductionmentioning

confidence: 99%

Collagen based film with well epithelial and stromal regeneration as corneal repair materials: Improving mechanical property by crosslinking with citric acid

Zhao

Liu

et al. 2015

Materials Science and Engineering: C

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“…87 The distinct features of collagen are its thrombogenicity, biodegradability, enzymatic degradability, biocompatibility, mechanical and biological properties. 88 They are used as haemostatic agents, 89 wound dressing agents, 90 in ophthalmology 91 and bone tissue engineering. 92 There are several Food and Drug Administration (FDA) approved products that contain collagen like Alloderm (for burn treatment), Apligraf 93 , Orcel (skin equivalents), haemostatic sealants and collagraft (bone graft substitute).…”

Section: Proteinsmentioning

confidence: 99%

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

et al. 2015

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Dermal tissue engineering focuses on the restoration of diseased and damaged tissues by using a combination of cells, biomaterials, and bioactive molecules. Inorganic substances like zeolites, clay, mesoporous silica, metals, and metal oxides are advanced materials used in wound healing research. They can improve the structural stability and bioactivity of bio polymeric scaffolds. Zeolites, clays, and mesoporous silica act as suitable carriers for drug delivery and when incorporated within scaffolds, serve as ideal matrices for promoting skin regeneration. This review focuses on various natural polymers/inorganic materials based composite scaffolds used for skin tissue engineering, highlighting their synthesis routes and mode of action by which wound healing is enhanced. Among the different inorganic materials used, the role of zeolites incorporated biocomposites for promoting blood coagulation, antibacterial effect; oxygen delivery to cells and wound healing are discussed in detail. The article thus includes recent attempts to explore the hidden potential of inorganic materials in dermal tissue engineering.

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Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Cited by 73 publications

References 23 publications

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Collagen based film with well epithelial and stromal regeneration as corneal repair materials: Improving mechanical property by crosslinking with citric acid

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

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